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Protein

Medium-chain specific acyl-CoA dehydrogenase, mitochondrial

Gene

ACADM

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme is specific for acyl chain lengths of 4 to 16.By similarity

Catalytic activityi

A medium-chain acyl-CoA + electron-transfer flavoprotein = a medium-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein.

Cofactori

FADBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei167 – 1671Substrate; via carbonyl oxygenBy similarity
Active sitei401 – 4011Proton acceptorBy similarity
Binding sitei402 – 4021Substrate; via amide nitrogenBy similarity
Binding sitei413 – 4131SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi158 – 16710FADBy similarity
Nucleotide bindingi191 – 1933FADBy similarity
Nucleotide bindingi306 – 3083FADBy similarity
Nucleotide bindingi316 – 3172FADBy similarity
Nucleotide bindingi374 – 3785FADBy similarity
Nucleotide bindingi403 – 4053FADBy similarity

GO - Molecular functioni

  1. acyl-CoA dehydrogenase activity Source: UniProtKB
  2. flavin adenine dinucleotide binding Source: InterPro
  3. medium-chain-acyl-CoA dehydrogenase activity Source: Ensembl

GO - Biological processi

  1. cardiac muscle cell differentiation Source: Ensembl
  2. carnitine metabolic process, CoA-linked Source: Ensembl
  3. fatty acid beta-oxidation Source: UniProtKB
  4. fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: Ensembl
  5. glycogen biosynthetic process Source: Ensembl
  6. liver development Source: Ensembl
  7. medium-chain fatty acid metabolic process Source: Ensembl
  8. post-embryonic development Source: Ensembl
  9. regulation of gluconeogenesis Source: Ensembl
  10. response to cold Source: Ensembl
  11. response to starvation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiREACT_299477. mitochondrial fatty acid beta-oxidation of unsaturated fatty acids.
REACT_313858. PPARA activates gene expression.
REACT_317946. Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA.
REACT_347067. Beta oxidation of octanoyl-CoA to hexanoyl-CoA.
SABIO-RKQ3SZB4.
UniPathwayiUPA00660.

Names & Taxonomyi

Protein namesi
Recommended name:
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (EC:1.3.8.7)
Short name:
MCAD
Gene namesi
Name:ACADM
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 3

Subcellular locationi

  1. Mitochondrion matrix By similarity

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2525MitochondrionBy similarityAdd
BLAST
Chaini26 – 421396Medium-chain specific acyl-CoA dehydrogenase, mitochondrialPRO_0000281995Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei69 – 691N6-acetyllysine; alternateBy similarity
Modified residuei69 – 691N6-succinyllysine; alternateBy similarity
Modified residuei79 – 791N6-acetyllysineBy similarity
Modified residuei179 – 1791N6-succinyllysineBy similarity
Modified residuei212 – 2121N6-acetyllysine; alternateBy similarity
Modified residuei212 – 2121N6-succinyllysine; alternateBy similarity
Modified residuei217 – 2171N6-acetyllysine; alternateBy similarity
Modified residuei217 – 2171N6-succinyllysine; alternateBy similarity
Modified residuei259 – 2591N6-acetyllysine; alternateBy similarity
Modified residuei259 – 2591N6-succinyllysine; alternateBy similarity
Modified residuei271 – 2711N6-acetyllysine; alternateBy similarity
Modified residuei271 – 2711N6-succinyllysine; alternateBy similarity
Modified residuei279 – 2791N6-acetyllysineBy similarity
Modified residuei301 – 3011N6-acetyllysineBy similarity

Post-translational modificationi

Acetylation at Lys-307 and Lys-311 in proximity of the cofactor-binding sites reduces catalytic activity. These sites are deacetylated by SIRT3 (By similarity).By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ3SZB4.
PRIDEiQ3SZB4.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ3SZB4.
SMRiQ3SZB4. Positions 36-420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni278 – 2814Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the acyl-CoA dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1960.
GeneTreeiENSGT00760000119007.
HOGENOMiHOG000131659.
HOVERGENiHBG000224.
InParanoidiQ3SZB4.
KOiK00249.
OMAiCLDVATQ.
OrthoDBiEOG74FF0S.
TreeFamiTF105020.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
2.40.110.10. 1 hit.
InterProiIPR006089. Acyl-CoA_DH_CS.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
PS00073. ACYL_COA_DH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SZB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIALFRRSCG VLRSLSHFDW RSQHTKTALQ REPGSGFSFE FTEQQKEFQA
60 70 80 90 100
TARKFAREEI IPLAAEYDKT GEYPVPLIKR AWELGLMNTH IPESCGGLGL
110 120 130 140 150
GTFDSCLISE ELAYGCTGVQ TAIEANSLGQ MPVIIAGNDQ QQKKYLGRMT
160 170 180 190 200
EEPLMCAYCV TEPVAGSDVA GIKTKAEKKG DEYIINGQKM WITNGGKANW
210 220 230 240 250
YFLLARSDPD PKAPASKAFT GFIVEADTPG VQIGRKELNM GQRCSDTRGI
260 270 280 290 300
VFEDVRVPKE NVLIGEGAGF KIAMGAFDKT RPPVAAAAVG LAQRALDEAT
310 320 330 340 350
KYALERKTFG KLLIEHQGIS FLLAEMAMKV ELARLSYQRA AWEVDSGRRN
360 370 380 390 400
TYYASIAKAY AGDIANQLAS DAVQIFGGNG FNTEYPVEKL MRDAKIYQIY
410 420
EGTAQIQRLI IAREHIGRYK K
Length:421
Mass (Da):46,573
Last modified:October 11, 2005 - v1
Checksum:iC43979D54E7A3A8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU009460 mRNA. Translation: ABS70460.1.
BC102989 mRNA. Translation: AAI02990.1.
RefSeqiNP_001068703.1. NM_001075235.1.
UniGeneiBt.61205.

Genome annotation databases

EnsembliENSBTAT00000033470; ENSBTAP00000033383; ENSBTAG00000024240.
GeneIDi505968.
KEGGibta:505968.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU009460 mRNA. Translation: ABS70460.1.
BC102989 mRNA. Translation: AAI02990.1.
RefSeqiNP_001068703.1. NM_001075235.1.
UniGeneiBt.61205.

3D structure databases

ProteinModelPortaliQ3SZB4.
SMRiQ3SZB4. Positions 36-420.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ3SZB4.
PRIDEiQ3SZB4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000033470; ENSBTAP00000033383; ENSBTAG00000024240.
GeneIDi505968.
KEGGibta:505968.

Organism-specific databases

CTDi34.

Phylogenomic databases

eggNOGiCOG1960.
GeneTreeiENSGT00760000119007.
HOGENOMiHOG000131659.
HOVERGENiHBG000224.
InParanoidiQ3SZB4.
KOiK00249.
OMAiCLDVATQ.
OrthoDBiEOG74FF0S.
TreeFamiTF105020.

Enzyme and pathway databases

UniPathwayiUPA00660.
ReactomeiREACT_299477. mitochondrial fatty acid beta-oxidation of unsaturated fatty acids.
REACT_313858. PPARA activates gene expression.
REACT_317946. Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA.
REACT_347067. Beta oxidation of octanoyl-CoA to hexanoyl-CoA.
SABIO-RKQ3SZB4.

Miscellaneous databases

NextBioi20867391.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
2.40.110.10. 1 hit.
InterProiIPR006089. Acyl-CoA_DH_CS.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
PS00073. ACYL_COA_DH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Association of bovine ACADM SNP with economic traits."
    Li H., Xu S., Gao X., Ren H., Li J.
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Heart ventricle.

Entry informationi

Entry nameiACADM_BOVIN
AccessioniPrimary (citable) accession number: Q3SZB4
Secondary accession number(s): A7LFV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 11, 2005
Last modified: April 1, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

A number of straight-chain acyl-CoA dehydrogenases of different substrate specificities are present in mammalian tissues.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.