Reviewed, UniProtKB/Swiss-Prot Q3SZB4 (ACADM_BOVIN)

Names and origin

Protein names

Recommended name:
    Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
      Short name=MCAD
    EC=1.3.99.3

Gene names

Name:

ACADM

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This enzyme is specific for acyl chain lengths of 4 to 16 By similarity.
Catalytic activity	Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor.
Cofactor	FAD By similarity.
Pathway	Lipid metabolism; mitochondrial fatty acid beta-oxidation.
Subunit structure	Homotetramer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Miscellaneous	A number of straight-chain acyl-CoA dehydrogenases of different substrate specificities are present in mammalian tissues.
Sequence similarities	Belongs to the acyl-CoA dehydrogenase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	fatty acid beta-oxidation Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro acyl-CoA dehydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 25	25	Mitochondrion By similarity
	Chain	26 – 421	396	Medium-chain specific acyl-CoA dehydrogenase, mitochondrial		PRO_0000281995
Regions
	Nucleotide binding	158 – 167	10	FAD By similarity
	Nucleotide binding	191 – 193	3	FAD By similarity
	Nucleotide binding	306 – 308	3	FAD By similarity
	Nucleotide binding	316 – 317	2	FAD By similarity
	Nucleotide binding	374 – 378	5	FAD By similarity
	Nucleotide binding	403 – 405	3	FAD By similarity
	Region	278 – 281	4	Substrate binding By similarity
Sites
	Active site	401	1	Proton acceptor By similarity
	Binding site	167	1	Substrate; via carbonyl oxygen By similarity
	Binding site	402	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SZB4-1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: C43979D54E7A3A8D	FASTA	421	46,573
	10 20 30 40 50 60 MIALFRRSCG VLRSLSHFDW RSQHTKTALQ REPGSGFSFE FTEQQKEFQA TARKFAREEI 70 80 90 100 110 120 IPLAAEYDKT GEYPVPLIKR AWELGLMNTH IPESCGGLGL GTFDSCLISE ELAYGCTGVQ 130 140 150 160 170 180 TAIEANSLGQ MPVIIAGNDQ QQKKYLGRMT EEPLMCAYCV TEPVAGSDVA GIKTKAEKKG 190 200 210 220 230 240 DEYIINGQKM WITNGGKANW YFLLARSDPD PKAPASKAFT GFIVEADTPG VQIGRKELNM 250 260 270 280 290 300 GQRCSDTRGI VFEDVRVPKE NVLIGEGAGF KIAMGAFDKT RPPVAAAAVG LAQRALDEAT 310 320 330 340 350 360 KYALERKTFG KLLIEHQGIS FLLAEMAMKV ELARLSYQRA AWEVDSGRRN TYYASIAKAY 370 380 390 400 410 420 AGDIANQLAS DAVQIFGGNG FNTEYPVEKL MRDAKIYQIY EGTAQIQRLI IAREHIGRYK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Association of bovine ACADM SNP with economic traits." Li H., Xu S., Gao X., Ren H., Li J. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Heart ventricle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	EU009460 mRNA. Translation: ABS70460.1. BC102989 mRNA. Translation: AAI02990.1.
IPI	IPI00704474.
RefSeq	NP_001068703.1.
UniGene	Bt.61205
3D structure databases
SMR	Q3SZB4. Positions 36-420.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000024240. Bos taurus. [Contig view]
GeneID	505968.
KEGG	bta:505968.
Phylogenomic databases
HOVERGEN	Q3SZB4.
OMA	Q3SZB4. EYPVPLI.
Enzyme and pathway databases
BRENDA	1.3.99.3. 251.
Family and domain databases
InterPro	IPR006089. Acyl-CoA_DH_CS. IPR006092. Acyl-CoA_DH_N. IPR006090. Acyl-CoA_Oxase/DH_1. IPR006091. Acyl-CoA_Oxase/DH_M. IPR013786. AcylCoA_DH/ox_N. IPR013764. AcylCoA_oxidase/DH_1/2_C. [Graphical view]
Gene3D	G3DSA:2.40.110.10. Acyl_CoA_DH/ox_M. 1 hit. G3DSA:1.10.540.10. AcylCoA_DH/ox_N. 1 hit. G3DSA:1.20.140.10. AcylCoA_DH_1/2_C. 1 hit.
Pfam	PF00441. Acyl-CoA_dh_1. 1 hit. PF02770. Acyl-CoA_dh_M. 1 hit. PF02771. Acyl-CoA_dh_N. 1 hit. [Graphical view]
PROSITE	PS00072. ACYL_COA_DH_1. 1 hit. PS00073. ACYL_COA_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACADM_BOVIN

Accession

Primary (citable) accession number: Q3SZB4
Secondary accession number(s): A7LFV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	October 11, 2005
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)