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Protein

Medium-chain specific acyl-CoA dehydrogenase, mitochondrial

Gene

ACADM

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acyl-CoA dehydrogenase specific for acyl chain lengths of 4 to 16 that catalyzes the initial step of fatty acid beta-oxidation. Utilizes the electron transfer flavoprotein (ETF) as an electron acceptor to transfer electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).By similarity

Catalytic activityi

A medium-chain acyl-CoA + electron-transfer flavoprotein = a medium-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein.

Cofactori

FADBy similarity

Pathwayi: mitochondrial fatty acid beta-oxidation

This protein is involved in the pathway mitochondrial fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway mitochondrial fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei167Substrate; via carbonyl oxygenBy similarity1
Active sitei401Proton acceptorBy similarity1
Binding sitei402Substrate; via amide nitrogenBy similarity1
Binding sitei413SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi158 – 167FADBy similarity10
Nucleotide bindingi191 – 193FADBy similarity3
Nucleotide bindingi306 – 308FADBy similarity3
Nucleotide bindingi316 – 317FADBy similarity2
Nucleotide bindingi374 – 378FADBy similarity5
Nucleotide bindingi403 – 405FADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-BTA-77288. mitochondrial fatty acid beta-oxidation of unsaturated fatty acids.
R-BTA-77346. Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA.
R-BTA-77348. Beta oxidation of octanoyl-CoA to hexanoyl-CoA.
SABIO-RKQ3SZB4.
UniPathwayiUPA00660.

Names & Taxonomyi

Protein namesi
Recommended name:
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (EC:1.3.8.7)
Short name:
MCAD
Gene namesi
Name:ACADM
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 25MitochondrionBy similarityAdd BLAST25
ChainiPRO_000028199526 – 421Medium-chain specific acyl-CoA dehydrogenase, mitochondrialAdd BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei69N6-acetyllysine; alternateBy similarity1
Modified residuei69N6-succinyllysine; alternateBy similarity1
Modified residuei79N6-acetyllysineBy similarity1
Modified residuei179N6-succinyllysineBy similarity1
Modified residuei212N6-acetyllysine; alternateBy similarity1
Modified residuei212N6-succinyllysine; alternateBy similarity1
Modified residuei217N6-acetyllysine; alternateBy similarity1
Modified residuei217N6-succinyllysine; alternateBy similarity1
Modified residuei259N6-acetyllysine; alternateBy similarity1
Modified residuei259N6-succinyllysine; alternateBy similarity1
Modified residuei271N6-acetyllysine; alternateBy similarity1
Modified residuei271N6-succinyllysine; alternateBy similarity1
Modified residuei279N6-acetyllysineBy similarity1
Modified residuei301N6-acetyllysineBy similarity1
Modified residuei351PhosphothreonineBy similarity1

Post-translational modificationi

Acetylation at Lys-307 and Lys-311 in proximity of the cofactor-binding sites reduces catalytic activity. These sites are deacetylated by SIRT3 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ3SZB4.
PeptideAtlasiQ3SZB4.
PRIDEiQ3SZB4.

Expressioni

Gene expression databases

BgeeiENSBTAG00000024240.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000033383.

Structurei

3D structure databases

ProteinModelPortaliQ3SZB4.
SMRiQ3SZB4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni278 – 281Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the acyl-CoA dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0140. Eukaryota.
COG1960. LUCA.
GeneTreeiENSGT00760000119007.
HOGENOMiHOG000131659.
HOVERGENiHBG000224.
InParanoidiQ3SZB4.
KOiK00249.
OMAiHIPENCD.
OrthoDBiEOG091G04BS.
TreeFamiTF105020.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
InterProiIPR006089. Acyl-CoA_DH_CS.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
PS00073. ACYL_COA_DH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SZB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIALFRRSCG VLRSLSHFDW RSQHTKTALQ REPGSGFSFE FTEQQKEFQA
60 70 80 90 100
TARKFAREEI IPLAAEYDKT GEYPVPLIKR AWELGLMNTH IPESCGGLGL
110 120 130 140 150
GTFDSCLISE ELAYGCTGVQ TAIEANSLGQ MPVIIAGNDQ QQKKYLGRMT
160 170 180 190 200
EEPLMCAYCV TEPVAGSDVA GIKTKAEKKG DEYIINGQKM WITNGGKANW
210 220 230 240 250
YFLLARSDPD PKAPASKAFT GFIVEADTPG VQIGRKELNM GQRCSDTRGI
260 270 280 290 300
VFEDVRVPKE NVLIGEGAGF KIAMGAFDKT RPPVAAAAVG LAQRALDEAT
310 320 330 340 350
KYALERKTFG KLLIEHQGIS FLLAEMAMKV ELARLSYQRA AWEVDSGRRN
360 370 380 390 400
TYYASIAKAY AGDIANQLAS DAVQIFGGNG FNTEYPVEKL MRDAKIYQIY
410 420
EGTAQIQRLI IAREHIGRYK K
Length:421
Mass (Da):46,573
Last modified:October 11, 2005 - v1
Checksum:iC43979D54E7A3A8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU009460 mRNA. Translation: ABS70460.1.
BC102989 mRNA. Translation: AAI02990.1.
RefSeqiNP_001068703.1. NM_001075235.1.
UniGeneiBt.61205.

Genome annotation databases

EnsembliENSBTAT00000033470; ENSBTAP00000033383; ENSBTAG00000024240.
GeneIDi505968.
KEGGibta:505968.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU009460 mRNA. Translation: ABS70460.1.
BC102989 mRNA. Translation: AAI02990.1.
RefSeqiNP_001068703.1. NM_001075235.1.
UniGeneiBt.61205.

3D structure databases

ProteinModelPortaliQ3SZB4.
SMRiQ3SZB4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000033383.

Proteomic databases

PaxDbiQ3SZB4.
PeptideAtlasiQ3SZB4.
PRIDEiQ3SZB4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000033470; ENSBTAP00000033383; ENSBTAG00000024240.
GeneIDi505968.
KEGGibta:505968.

Organism-specific databases

CTDi34.

Phylogenomic databases

eggNOGiKOG0140. Eukaryota.
COG1960. LUCA.
GeneTreeiENSGT00760000119007.
HOGENOMiHOG000131659.
HOVERGENiHBG000224.
InParanoidiQ3SZB4.
KOiK00249.
OMAiHIPENCD.
OrthoDBiEOG091G04BS.
TreeFamiTF105020.

Enzyme and pathway databases

UniPathwayiUPA00660.
ReactomeiR-BTA-77288. mitochondrial fatty acid beta-oxidation of unsaturated fatty acids.
R-BTA-77346. Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA.
R-BTA-77348. Beta oxidation of octanoyl-CoA to hexanoyl-CoA.
SABIO-RKQ3SZB4.

Gene expression databases

BgeeiENSBTAG00000024240.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
InterProiIPR006089. Acyl-CoA_DH_CS.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
PS00073. ACYL_COA_DH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACADM_BOVIN
AccessioniPrimary (citable) accession number: Q3SZB4
Secondary accession number(s): A7LFV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

A number of straight-chain acyl-CoA dehydrogenases of different substrate specificities are present in mammalian tissues.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.