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Q3SZB4

- ACADM_BOVIN

UniProt

Q3SZB4 - ACADM_BOVIN

Protein

Medium-chain specific acyl-CoA dehydrogenase, mitochondrial

Gene

ACADM

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (11 Oct 2005)
      Previous versions | rss
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    Functioni

    This enzyme is specific for acyl chain lengths of 4 to 16.By similarity

    Catalytic activityi

    A medium-chain acyl-CoA + electron-transfer flavoprotein = a medium-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein.

    Cofactori

    FAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei167 – 1671Substrate; via carbonyl oxygenBy similarity
    Active sitei401 – 4011Proton acceptorBy similarity
    Binding sitei402 – 4021Substrate; via amide nitrogenBy similarity
    Binding sitei413 – 4131SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi158 – 16710FADBy similarity
    Nucleotide bindingi191 – 1933FADBy similarity
    Nucleotide bindingi306 – 3083FADBy similarity
    Nucleotide bindingi316 – 3172FADBy similarity
    Nucleotide bindingi374 – 3785FADBy similarity
    Nucleotide bindingi403 – 4053FADBy similarity

    GO - Molecular functioni

    1. acyl-CoA dehydrogenase activity Source: UniProtKB
    2. flavin adenine dinucleotide binding Source: InterPro
    3. medium-chain-acyl-CoA dehydrogenase activity Source: Ensembl

    GO - Biological processi

    1. cardiac muscle cell differentiation Source: Ensembl
    2. carnitine biosynthetic process Source: Ensembl
    3. carnitine metabolic process, CoA-linked Source: Ensembl
    4. fatty acid beta-oxidation Source: UniProtKB
    5. fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: Ensembl
    6. glycogen biosynthetic process Source: Ensembl
    7. liver development Source: Ensembl
    8. medium-chain fatty acid catabolic process Source: Ensembl
    9. post-embryonic development Source: Ensembl
    10. regulation of gluconeogenesis Source: Ensembl
    11. response to cold Source: Ensembl
    12. response to starvation Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    ReactomeiREACT_207237. PPARA activates gene expression.
    REACT_211406. Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA.
    REACT_215287. mitochondrial fatty acid beta-oxidation of unsaturated fatty acids.
    REACT_220229. Beta oxidation of octanoyl-CoA to hexanoyl-CoA.
    SABIO-RKQ3SZB4.
    UniPathwayiUPA00660.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (EC:1.3.8.7)
    Short name:
    MCAD
    Gene namesi
    Name:ACADM
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 3

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. axon Source: Ensembl
    2. mitochondrial matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2525MitochondrionBy similarityAdd
    BLAST
    Chaini26 – 421396Medium-chain specific acyl-CoA dehydrogenase, mitochondrialPRO_0000281995Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei69 – 691N6-acetyllysine; alternateBy similarity
    Modified residuei69 – 691N6-succinyllysine; alternateBy similarity
    Modified residuei79 – 791N6-acetyllysineBy similarity
    Modified residuei179 – 1791N6-succinyllysineBy similarity
    Modified residuei212 – 2121N6-acetyllysine; alternateBy similarity
    Modified residuei212 – 2121N6-succinyllysine; alternateBy similarity
    Modified residuei217 – 2171N6-acetyllysine; alternateBy similarity
    Modified residuei217 – 2171N6-succinyllysine; alternateBy similarity
    Modified residuei259 – 2591N6-acetyllysine; alternateBy similarity
    Modified residuei259 – 2591N6-succinyllysine; alternateBy similarity
    Modified residuei271 – 2711N6-acetyllysine; alternateBy similarity
    Modified residuei271 – 2711N6-succinyllysine; alternateBy similarity
    Modified residuei279 – 2791N6-acetyllysineBy similarity
    Modified residuei301 – 3011N6-acetyllysineBy similarity

    Post-translational modificationi

    Acetylation at Lys-307 and Lys-311 in proximity of the cofactor-binding sites reduces catalytic activity. These sites are deacetylated by SIRT3 By similarity.By similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ3SZB4.
    PRIDEiQ3SZB4.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ3SZB4.
    SMRiQ3SZB4. Positions 36-420.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni278 – 2814Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the acyl-CoA dehydrogenase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1960.
    GeneTreeiENSGT00750000117417.
    HOGENOMiHOG000131659.
    HOVERGENiHBG000224.
    InParanoidiQ3SZB4.
    KOiK00249.
    OMAiSAWEVDQ.
    OrthoDBiEOG74FF0S.
    TreeFamiTF105020.

    Family and domain databases

    Gene3Di1.10.540.10. 1 hit.
    2.40.110.10. 1 hit.
    InterProiIPR006089. Acyl-CoA_DH_CS.
    IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
    IPR009075. AcylCo_DH/oxidase_C.
    IPR013786. AcylCoA_DH/ox_N.
    IPR009100. AcylCoA_DH/oxidase_NM_dom.
    [Graphical view]
    PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
    PF02770. Acyl-CoA_dh_M. 1 hit.
    PF02771. Acyl-CoA_dh_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47203. SSF47203. 1 hit.
    SSF56645. SSF56645. 1 hit.
    PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
    PS00073. ACYL_COA_DH_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q3SZB4-1 [UniParc]FASTAAdd to Basket

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    MIALFRRSCG VLRSLSHFDW RSQHTKTALQ REPGSGFSFE FTEQQKEFQA    50
    TARKFAREEI IPLAAEYDKT GEYPVPLIKR AWELGLMNTH IPESCGGLGL 100
    GTFDSCLISE ELAYGCTGVQ TAIEANSLGQ MPVIIAGNDQ QQKKYLGRMT 150
    EEPLMCAYCV TEPVAGSDVA GIKTKAEKKG DEYIINGQKM WITNGGKANW 200
    YFLLARSDPD PKAPASKAFT GFIVEADTPG VQIGRKELNM GQRCSDTRGI 250
    VFEDVRVPKE NVLIGEGAGF KIAMGAFDKT RPPVAAAAVG LAQRALDEAT 300
    KYALERKTFG KLLIEHQGIS FLLAEMAMKV ELARLSYQRA AWEVDSGRRN 350
    TYYASIAKAY AGDIANQLAS DAVQIFGGNG FNTEYPVEKL MRDAKIYQIY 400
    EGTAQIQRLI IAREHIGRYK K 421
    Length:421
    Mass (Da):46,573
    Last modified:October 11, 2005 - v1
    Checksum:iC43979D54E7A3A8D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU009460 mRNA. Translation: ABS70460.1.
    BC102989 mRNA. Translation: AAI02990.1.
    RefSeqiNP_001068703.1. NM_001075235.1.
    UniGeneiBt.61205.

    Genome annotation databases

    EnsembliENSBTAT00000033470; ENSBTAP00000033383; ENSBTAG00000024240.
    GeneIDi505968.
    KEGGibta:505968.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU009460 mRNA. Translation: ABS70460.1 .
    BC102989 mRNA. Translation: AAI02990.1 .
    RefSeqi NP_001068703.1. NM_001075235.1.
    UniGenei Bt.61205.

    3D structure databases

    ProteinModelPortali Q3SZB4.
    SMRi Q3SZB4. Positions 36-420.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q3SZB4.
    PRIDEi Q3SZB4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000033470 ; ENSBTAP00000033383 ; ENSBTAG00000024240 .
    GeneIDi 505968.
    KEGGi bta:505968.

    Organism-specific databases

    CTDi 34.

    Phylogenomic databases

    eggNOGi COG1960.
    GeneTreei ENSGT00750000117417.
    HOGENOMi HOG000131659.
    HOVERGENi HBG000224.
    InParanoidi Q3SZB4.
    KOi K00249.
    OMAi SAWEVDQ.
    OrthoDBi EOG74FF0S.
    TreeFami TF105020.

    Enzyme and pathway databases

    UniPathwayi UPA00660 .
    Reactomei REACT_207237. PPARA activates gene expression.
    REACT_211406. Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA.
    REACT_215287. mitochondrial fatty acid beta-oxidation of unsaturated fatty acids.
    REACT_220229. Beta oxidation of octanoyl-CoA to hexanoyl-CoA.
    SABIO-RK Q3SZB4.

    Miscellaneous databases

    NextBioi 20867391.

    Family and domain databases

    Gene3Di 1.10.540.10. 1 hit.
    2.40.110.10. 1 hit.
    InterProi IPR006089. Acyl-CoA_DH_CS.
    IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
    IPR009075. AcylCo_DH/oxidase_C.
    IPR013786. AcylCoA_DH/ox_N.
    IPR009100. AcylCoA_DH/oxidase_NM_dom.
    [Graphical view ]
    Pfami PF00441. Acyl-CoA_dh_1. 1 hit.
    PF02770. Acyl-CoA_dh_M. 1 hit.
    PF02771. Acyl-CoA_dh_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47203. SSF47203. 1 hit.
    SSF56645. SSF56645. 1 hit.
    PROSITEi PS00072. ACYL_COA_DH_1. 1 hit.
    PS00073. ACYL_COA_DH_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Association of bovine ACADM SNP with economic traits."
      Li H., Xu S., Gao X., Ren H., Li J.
      Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Heart ventricle.

    Entry informationi

    Entry nameiACADM_BOVIN
    AccessioniPrimary (citable) accession number: Q3SZB4
    Secondary accession number(s): A7LFV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    A number of straight-chain acyl-CoA dehydrogenases of different substrate specificities are present in mammalian tissues.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3