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Protein

Polycomb protein EED

Gene

EED

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark (By similarity). The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-BTA-212300. PRC2 methylates histones and DNA.
R-BTA-2559580. Oxidative Stress Induced Senescence.

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb protein EED
Gene namesi
Name:EED
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 29

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 441440Polycomb protein EEDPRO_0000343724Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei34 – 341PhosphoserineBy similarity
Modified residuei55 – 551PhosphothreonineBy similarity
Modified residuei66 – 661N6,N6,N6-trimethyllysine; alternateBy similarity
Modified residuei66 – 661N6,N6-dimethyllysine; alternateBy similarity
Modified residuei66 – 661N6-methyllysine; alternateBy similarity
Modified residuei197 – 1971N6,N6,N6-trimethyllysine; alternateBy similarity
Modified residuei197 – 1971N6,N6-dimethyllysine; alternateBy similarity
Modified residuei197 – 1971N6-methyllysine; alternateBy similarity
Modified residuei268 – 2681N6,N6,N6-trimethyllysine; alternateBy similarity
Modified residuei268 – 2681N6,N6-dimethyllysine; alternateBy similarity
Modified residuei268 – 2681N6-methyllysine; alternateBy similarity
Modified residuei284 – 2841N6,N6,N6-trimethyllysine; alternateBy similarity
Modified residuei284 – 2841N6,N6-dimethyllysine; alternateBy similarity
Modified residuei284 – 2841N6-methyllysine; alternateBy similarity

Post-translational modificationi

Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines (By similarity).By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ3SZ25.

Interactioni

Subunit structurei

Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2 (By similarity). The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2 (By similarity). The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit (By similarity). Interacts with HDAC, HDAC2, histone H1, KMT2A/MLL1 and YY1 (By similarity). May interact with ITGA4, ITGAE and ITGB7 (By similarity). Interacts with CDYL. Interacts with ARNTL/BMAL1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010324.

Structurei

3D structure databases

ProteinModelPortaliQ3SZ25.
SMRiQ3SZ25. Positions 82-440.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati91 – 13444WD 1Add
BLAST
Repeati142 – 18544WD 2Add
BLAST
Repeati188 – 22841WD 3Add
BLAST
Repeati234 – 27542WD 4Add
BLAST
Repeati304 – 34138WD 5Add
BLAST
Repeati359 – 39941WD 6Add
BLAST
Repeati408 – 44134WD 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni81 – 441361Interaction with EZH2By similarityAdd
BLAST

Domaini

The WD repeat domain mediates recognition of trimethylated histone peptides at the consensus sequence Ala-Arg-Lys-Ser. This is achieved through an aromatic cage encircling the methyllysine, and involving Phe-97, Tyr-148 and Tyr-365 (By similarity).By similarity

Sequence similaritiesi

Belongs to the WD repeat ESC family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1034. Eukaryota.
ENOG410XRQI. LUCA.
GeneTreeiENSGT00510000047334.
HOGENOMiHOG000005759.
HOVERGENiHBG052708.
InParanoidiQ3SZ25.
KOiK11462.
OMAiCTTLTHP.
OrthoDBiEOG7WQ7S8.
TreeFamiTF314451.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SZ25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEREVSTVP AGTDMPAAKK QKLSSDENSN PDLSGDENDD AVSIESGTNT
60 70 80 90 100
ERPDTPTNTP NAPGRKSWGK GKWKSKKCKY SFKCVNSLKE DHNQPLFGVQ
110 120 130 140 150
FNWHSKEGDP LVFATVGSNR VTLYECHSQG EIRLLQSYVD ADADENFYTC
160 170 180 190 200
AWTYDSNTSH PLLAVAGSRG IIRIINPITM QCIKHYVGHG NAINELKFHP
210 220 230 240 250
RDPNLLLSVS KDHALRLWNI QTDTLVAIFG GVEGHRDEVL SADYDLLGEK
260 270 280 290 300
IMSCGMDHSL KLWRINSKRM MNAIKESYDY NPNKTNRPFI SQKIHFPDFS
310 320 330 340 350
TRDIHRNYVD CVRWLGDLIL SKSCENAIVC WKPGKMEDDI DKIKPSESNV
360 370 380 390 400
TILGRFDYSQ CDIWYMRFSM DFWQKMLALG NQVGKLYVWD LEVEDPHKAK
410 420 430 440
CTTLTHHKCG AAIRQTSFSR DSSILIAVCD DASIWRWDRL R
Length:441
Mass (Da):50,226
Last modified:October 11, 2005 - v1
Checksum:iB4DA10D346BCA05F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC103217 mRNA. Translation: AAI03218.1.
RefSeqiNP_001035584.1. NM_001040494.2.
UniGeneiBt.49104.

Genome annotation databases

EnsembliENSBTAT00000010324; ENSBTAP00000010324; ENSBTAG00000007847.
GeneIDi404183.
KEGGibta:404183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC103217 mRNA. Translation: AAI03218.1.
RefSeqiNP_001035584.1. NM_001040494.2.
UniGeneiBt.49104.

3D structure databases

ProteinModelPortaliQ3SZ25.
SMRiQ3SZ25. Positions 82-440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010324.

Proteomic databases

PaxDbiQ3SZ25.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000010324; ENSBTAP00000010324; ENSBTAG00000007847.
GeneIDi404183.
KEGGibta:404183.

Organism-specific databases

CTDi8726.

Phylogenomic databases

eggNOGiKOG1034. Eukaryota.
ENOG410XRQI. LUCA.
GeneTreeiENSGT00510000047334.
HOGENOMiHOG000005759.
HOVERGENiHBG052708.
InParanoidiQ3SZ25.
KOiK11462.
OMAiCTTLTHP.
OrthoDBiEOG7WQ7S8.
TreeFamiTF314451.

Enzyme and pathway databases

ReactomeiR-BTA-212300. PRC2 methylates histones and DNA.
R-BTA-2559580. Oxidative Stress Induced Senescence.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.

Entry informationi

Entry nameiEED_BOVIN
AccessioniPrimary (citable) accession number: Q3SZ25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 11, 2005
Last modified: July 6, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.