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Q3SZ18

- HPRT_BOVIN

UniProt

Q3SZ18 - HPRT_BOVIN

Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

HPRT1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway By similarity.By similarity

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
    GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691GMPBy similarity
    Active sitei138 – 1381Proton acceptorBy similarity
    Binding sitei166 – 1661GMPBy similarity
    Metal bindingi194 – 1941MagnesiumBy similarity
    Binding sitei194 – 1941GMP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi134 – 1429GMPBy similarity
    Nucleotide bindingi186 – 1883GMPBy similarity

    GO - Molecular functioni

    1. guanine phosphoribosyltransferase activity Source: UniProtKB
    2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB
    3. magnesium ion binding Source: UniProtKB
    4. nucleotide binding Source: UniProtKB-KW
    5. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. adenine salvage Source: RefGenome
    2. GMP catabolic process Source: UniProtKB
    3. GMP salvage Source: RefGenome
    4. guanine salvage Source: UniProtKB
    5. hypoxanthine metabolic process Source: UniProtKB
    6. hypoxanthine salvage Source: UniProtKB
    7. IMP metabolic process Source: UniProtKB
    8. IMP salvage Source: RefGenome
    9. positive regulation of dopamine metabolic process Source: UniProtKB
    10. protein homotetramerization Source: UniProtKB
    11. purine nucleotide biosynthetic process Source: UniProtKB
    12. purine ribonucleoside salvage Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_211472. Purine salvage.
    UniPathwayiUPA00591; UER00648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HGPRT
    Short name:
    HGPRTase
    Gene namesi
    Name:HPRT1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome X

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. cytosol Source: RefGenome

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 218217Hypoxanthine-guanine phosphoribosyltransferasePRO_0000257812Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei103 – 1031N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ3SZ18.
    PRIDEiQ3SZ18.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000053015.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3SZ18.
    SMRiQ3SZ18. Positions 5-218.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0634.
    GeneTreeiENSGT00390000017323.
    HOGENOMiHOG000236521.
    HOVERGENiHBG000242.
    KOiK00760.
    OMAiAREIMKG.
    OrthoDBiEOG7673CK.
    TreeFamiTF313367.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    InterProiIPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q3SZ18-1 [UniParc]FASTAAdd to Basket

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    MAARSPSVVI SDDEPGYDLN LFCIPNHYAE DLEKVFIPHG LIMDRTERLA    50
    RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD KSIPMTVDFI 100
    RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLALV 150
    KKHKPKMVKV ASLLMKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR 200
    DLNHVCVISE TGKAKYKA 218
    Length:218
    Mass (Da):24,498
    Last modified:January 23, 2007 - v3
    Checksum:i761695098A5B6A20
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC103248 mRNA. Translation: AAI03249.1.
    RefSeqiNP_001029207.1. NM_001034035.2.
    UniGeneiBt.49238.

    Genome annotation databases

    EnsembliENSBTAT00000019547; ENSBTAP00000019547; ENSBTAG00000014685.
    GeneIDi281229.
    KEGGibta:281229.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC103248 mRNA. Translation: AAI03249.1 .
    RefSeqi NP_001029207.1. NM_001034035.2.
    UniGenei Bt.49238.

    3D structure databases

    ProteinModelPortali Q3SZ18.
    SMRi Q3SZ18. Positions 5-218.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000053015.

    Proteomic databases

    PaxDbi Q3SZ18.
    PRIDEi Q3SZ18.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000019547 ; ENSBTAP00000019547 ; ENSBTAG00000014685 .
    GeneIDi 281229.
    KEGGi bta:281229.

    Organism-specific databases

    CTDi 3251.

    Phylogenomic databases

    eggNOGi COG0634.
    GeneTreei ENSGT00390000017323.
    HOGENOMi HOG000236521.
    HOVERGENi HBG000242.
    KOi K00760.
    OMAi AREIMKG.
    OrthoDBi EOG7673CK.
    TreeFami TF313367.

    Enzyme and pathway databases

    UniPathwayi UPA00591 ; UER00648 .
    Reactomei REACT_211472. Purine salvage.

    Miscellaneous databases

    NextBioi 20805276.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    InterProi IPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal liver.

    Entry informationi

    Entry nameiHPRT_BOVIN
    AccessioniPrimary (citable) accession number: Q3SZ18
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 70 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3