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Protein

Transcription elongation factor SPT4

Gene

SUPT4H1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri16 – 3621C4-typeSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-BTA-112382. Formation of RNA Pol II elongation complex.
R-BTA-113418. Formation of the Early Elongation Complex.
R-BTA-674695. RNA Polymerase II Pre-transcription Events.
R-BTA-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-BTA-75955. RNA Polymerase II Transcription Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor SPT4
Alternative name(s):
DRB sensitivity-inducing factor small subunit
Short name:
DSIF small subunit
Gene namesi
Name:SUPT4H1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 117116Transcription elongation factor SPT4PRO_0000246082Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ3SYX6.

Expressioni

Gene expression databases

BgeeiENSBTAG00000003386.

Interactioni

Subunit structurei

Interacts with SUPT5H to form DSIF. DSIF interacts with the positive transcription elongation factor b complex (P-TEFb complex), which is composed of CDK9 and cyclin-T (CCNT1 or CCNT2). DSIF interacts with RNA polymerase II, and this interaction is reduced by phosphorylation of the C-terminal domain (CTD) of POLR2A by P-TEFb. DSIF also interacts with the NELF complex, which is composed of WHSC2/NELFA, COBRA1/NELFB, TH1L/NELFD and RDBP/NELFE, and this interaction occurs following prior binding of DSIF to RNA polymerase II. DSIF also interacts with HRMT1L2/PRMT1, HTATSF1/TATSF1, RNGTT/CAP1A, SKB1/PRMT5, SUPT6H, and can interact with PIN1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004389.

Structurei

3D structure databases

ProteinModelPortaliQ3SYX6.
SMRiQ3SYX6. Positions 2-117.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 4039Interaction with SUPT5HBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the SPT4 family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri16 – 3621C4-typeSequence analysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3490. Eukaryota.
COG5204. LUCA.
GeneTreeiENSGT00390000018559.
HOGENOMiHOG000172685.
HOVERGENiHBG055988.
InParanoidiQ3SYX6.
KOiK15171.
OMAiCDNCDEF.
OrthoDBiEOG091G0NIU.
TreeFamiTF300105.

Family and domain databases

CDDicd07973. Spt4. 1 hit.
InterProiIPR029040. RPABC4/Spt4.
IPR009287. Spt4.
IPR016046. Spt4-like.
IPR022800. Spt4/RpoE2_Znf.
[Graphical view]
PANTHERiPTHR12882. PTHR12882. 1 hit.
PfamiPF06093. Spt4. 1 hit.
[Graphical view]
PIRSFiPIRSF025023. Spt4. 1 hit.
SMARTiSM01389. Spt4. 1 hit.
[Graphical view]
SUPFAMiSSF63393. SSF63393. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SYX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALETVPKDL RHLRACLLCS LVKTIDQFEY DGCDNCDAYL QMKGNREMVY
60 70 80 90 100
DCTSSSFDGI IAMMSPEDSW VSKWQRVSNF KPGVYAVSVT GRLPQGIVRE
110
LKSRGVAYKS RDTAIKT
Length:117
Mass (Da):13,193
Last modified:October 11, 2005 - v1
Checksum:i09EDF007501D0F03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT025461 mRNA. Translation: ABF57417.1.
BC103340 mRNA. Translation: AAI03341.1.
RefSeqiNP_001029964.1. NM_001034792.2.
UniGeneiBt.49115.

Genome annotation databases

EnsembliENSBTAT00000004389; ENSBTAP00000004389; ENSBTAG00000003386.
GeneIDi616425.
KEGGibta:616425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT025461 mRNA. Translation: ABF57417.1.
BC103340 mRNA. Translation: AAI03341.1.
RefSeqiNP_001029964.1. NM_001034792.2.
UniGeneiBt.49115.

3D structure databases

ProteinModelPortaliQ3SYX6.
SMRiQ3SYX6. Positions 2-117.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004389.

Proteomic databases

PaxDbiQ3SYX6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000004389; ENSBTAP00000004389; ENSBTAG00000003386.
GeneIDi616425.
KEGGibta:616425.

Organism-specific databases

CTDi6827.

Phylogenomic databases

eggNOGiKOG3490. Eukaryota.
COG5204. LUCA.
GeneTreeiENSGT00390000018559.
HOGENOMiHOG000172685.
HOVERGENiHBG055988.
InParanoidiQ3SYX6.
KOiK15171.
OMAiCDNCDEF.
OrthoDBiEOG091G0NIU.
TreeFamiTF300105.

Enzyme and pathway databases

ReactomeiR-BTA-112382. Formation of RNA Pol II elongation complex.
R-BTA-113418. Formation of the Early Elongation Complex.
R-BTA-674695. RNA Polymerase II Pre-transcription Events.
R-BTA-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-BTA-75955. RNA Polymerase II Transcription Elongation.

Gene expression databases

BgeeiENSBTAG00000003386.

Family and domain databases

CDDicd07973. Spt4. 1 hit.
InterProiIPR029040. RPABC4/Spt4.
IPR009287. Spt4.
IPR016046. Spt4-like.
IPR022800. Spt4/RpoE2_Znf.
[Graphical view]
PANTHERiPTHR12882. PTHR12882. 1 hit.
PfamiPF06093. Spt4. 1 hit.
[Graphical view]
PIRSFiPIRSF025023. Spt4. 1 hit.
SMARTiSM01389. Spt4. 1 hit.
[Graphical view]
SUPFAMiSSF63393. SSF63393. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSPT4H_BOVIN
AccessioniPrimary (citable) accession number: Q3SYX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 11, 2005
Last modified: September 7, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.