Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Poly(ADP-ribose) glycohydrolase ARH3

Gene

ADPRHL2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells. Generates ADP-ribose from poly-(ADP-ribose), but does not hydrolyze ADP-ribose-arginine, -cysteine, -diphthamide, or -asparagine bonds. Due to catalytic inactivity of PARG mitochondrial isoforms, ARH3 is the only PAR hydrolyzing enzyme in mitochondria (By similarity).By similarity

Catalytic activityi

Hydrolyzes poly(ADP-D-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-D-ribose.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi42 – 421Magnesium 2By similarity
Metal bindingi77 – 771Magnesium 1By similarity
Metal bindingi78 – 781Magnesium 1By similarity
Metal bindingi79 – 791Magnesium 1By similarity
Binding sitei255 – 2551SubstrateBy similarity
Metal bindingi315 – 3151Magnesium 2By similarity
Metal bindingi317 – 3171Magnesium 1By similarity
Metal bindingi317 – 3171Magnesium 2By similarity
Metal bindingi318 – 3181Magnesium 2By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(ADP-ribose) glycohydrolase ARH3 (EC:3.2.1.143)
Alternative name(s):
ADP-ribosylhydrolase 3
[Protein ADP-ribosylarginine] hydrolase-like protein 2
Gene namesi
Name:ADPRHL2
Synonyms:ARH3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Cytoplasm By similarity
  • Mitochondrion matrix By similarity
  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2828MitochondrionSequence analysisAdd
BLAST
Chaini29 – 365337Poly(ADP-ribose) glycohydrolase ARH3PRO_0000277612Add
BLAST

Proteomic databases

PaxDbiQ3SYV9.
PRIDEiQ3SYV9.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000028950.

Structurei

3D structure databases

ProteinModelPortaliQ3SYV9.
SMRiQ3SYV9. Positions 19-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 65Poly-Ala

Sequence similaritiesi

Belongs to the ADP-ribosylglycohydrolase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IGSE. Eukaryota.
COG1397. LUCA.
HOGENOMiHOG000225333.
HOVERGENiHBG080863.
InParanoidiQ3SYV9.
KOiK11687.

Family and domain databases

InterProiIPR005502. Ribosyl_crysJ1.
[Graphical view]
PfamiPF03747. ADP_ribosyl_GH. 1 hit.
[Graphical view]
SUPFAMiSSF101478. SSF101478. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SYV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAAAMTAA GCGGAGAARS LSRFRGCLAG ALLGDCVGAV YEARDTVDLT
60 70 80 90 100
SVLRQVQDLE PDPGSPGSAR TEALCYTDDT AMARALVQSL LAKEAFDEVD
110 120 130 140 150
MAHRFAQEYK KDPDRGYGAG VITVFRKHLS PRCRDVFEPA RAQFNGKGSY
160 170 180 190 200
GNGGAMRVAG ISLAYSSVQD VQKFARLSAQ LTHASSLGYN GAILQALAVH
210 220 230 240 250
LALQGESSSE HFLEQLLGHM EELESDAQSV LDARELGMEE RPYSSRLKKI
260 270 280 290 300
GELLEQDSVT REEVVSELGN GIAAFESVPT AIYCFLRCME PDPEIPSTFN
310 320 330 340 350
SLQRTLVYSI SLGGDTDTIA TMAGAIAGAY YGMEQVPESW QQSCEGYEET
360
DVLAQSLHRV FQKSL
Length:365
Mass (Da):39,221
Last modified:October 11, 2005 - v1
Checksum:i59F65453AABF2802
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC103360 mRNA. Translation: AAI03361.1.
RefSeqiNP_001030417.1. NM_001035340.2.
UniGeneiBt.51810.

Genome annotation databases

GeneIDi521650.
KEGGibta:521650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC103360 mRNA. Translation: AAI03361.1.
RefSeqiNP_001030417.1. NM_001035340.2.
UniGeneiBt.51810.

3D structure databases

ProteinModelPortaliQ3SYV9.
SMRiQ3SYV9. Positions 19-364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000028950.

Proteomic databases

PaxDbiQ3SYV9.
PRIDEiQ3SYV9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi521650.
KEGGibta:521650.

Organism-specific databases

CTDi54936.

Phylogenomic databases

eggNOGiENOG410IGSE. Eukaryota.
COG1397. LUCA.
HOGENOMiHOG000225333.
HOVERGENiHBG080863.
InParanoidiQ3SYV9.
KOiK11687.

Family and domain databases

InterProiIPR005502. Ribosyl_crysJ1.
[Graphical view]
PfamiPF03747. ADP_ribosyl_GH. 1 hit.
[Graphical view]
SUPFAMiSSF101478. SSF101478. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARHL2_BOVIN
AccessioniPrimary (citable) accession number: Q3SYV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: October 11, 2005
Last modified: June 8, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.