ID   NCPR_BOVIN              Reviewed;         678 AA.
AC   Q3SYT8;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   16-JUN-2009, entry version 36.
DE   RecName: Full=NADPH--cytochrome P450 reductase;
DE            Short=CPR;
DE            Short=P450R;
DE            EC=1.6.2.4;
GN   Name=POR;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: This enzyme is required for electron transfer from NADP
CC       to cytochrome P450 in microsomes. It can also provide electron
CC       transfer to heme oxygenase and cytochrome B5 (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADPH + n oxidized hemoprotein = NADP(+) + n
CC       reduced hemoprotein.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- COFACTOR: FMN (By similarity).
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral
CC       membrane protein. Note=Anchored to the ER membrane by its N-
CC       terminal hydrophobic region (By similarity).
CC   -!- SIMILARITY: In the C-terminal section; belongs to the flavoprotein
CC       pyridine nucleotide cytochrome reductase family.
CC   -!- SIMILARITY: Contains 1 FAD-binding FR-type domain.
CC   -!- SIMILARITY: Contains 1 flavodoxin-like domain.
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DR   EMBL; BC103399; AAI03400.1; ALT_INIT; mRNA.
DR   IPI; IPI00696566; -.
DR   RefSeq; NP_001030467.1; -.
DR   UniGene; Bt.41220; -.
DR   SMR; Q3SYT8; 64-678.
DR   Ensembl; ENSBTAG00000017082; Bos taurus.
DR   GeneID; 532512; -.
DR   KEGG; bta:532512; -.
DR   HOVERGEN; Q3SYT8; -.
DR   BRENDA; 1.6.2.4; 251.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0003958; F:NADPH-hemoprotein reductase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR003097; FAD-binding_1.
DR   InterPro; IPR017927; Fd_Rdtase_FAD-bd.
DR   InterPro; IPR001094; Flavdoxin-like.
DR   InterPro; IPR008254; Flavodoxin/NO_synth.
DR   InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR   InterPro; IPR015702; NADPH_Cyt_P450_Rdtase.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD_bd.
DR   PANTHER; PTHR19384:SF17; NADPH_Cyt_Red; 1.
DR   Pfam; PF00667; FAD_binding_1; 1.
DR   Pfam; PF00258; Flavodoxin_1; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   PRINTS; PR00369; FLAVODOXIN.
DR   PRINTS; PR00371; FPNCR.
DR   PROSITE; PS51384; FAD_FR; 1.
DR   PROSITE; PS50902; FLAVODOXIN_LIKE; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; FAD; Flavoprotein; FMN; Membrane; NADP;
KW   Oxidoreductase; Phosphoprotein.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    678       NADPH--cytochrome P450 reductase.
FT                                /FTId=PRO_0000245573.
FT   DOMAIN       80    224       Flavodoxin-like.
FT   DOMAIN      279    521       FAD-binding FR-type.
FT   NP_BIND     170    201       FMN (By similarity).
FT   NP_BIND     314    325       FAD (By similarity).
FT   NP_BIND     451    461       FAD (By similarity).
FT   NP_BIND     529    547       NADP (By similarity).
FT   NP_BIND     624    640       NADP (By similarity).
FT   MOD_RES     575    575       Phosphotyrosine (By similarity).
SQ   SEQUENCE   678 AA;  77021 MW;  C4103BC020D8842C CRC64;
     MADSNMDAGT TTSEMVAEEV SLFSTTDVIL FSLIVGVMTY WFLFRKKKEE VPEFTKIQTT
     TSSVKDRSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK DAHRYGMRGM AADPEEYDLA
     DLSSLPEIEK ALAIFCMATY GEGDPTDNAQ DFYDWLQETD VDLSGVKYAV FALGNKTYEH
     FNAMGKYVDK RLEQLGAQRI FDLGLGDDDG NLEEDFITWR EQFWPAVCEH FGVEATGEES
     SIRQYELMVH TDMDMAKVYT GEMGRLKSYE NQKPPFDAKN PFLAVVTTNR KLNQGTERHL
     MHLELDISDS KIRYESGDHV AVYPANDSAL VNQLGEILGA DLDIIMSLNN LDEESNKKHP
     FPCPTSYRTA LTYYLDITNP PRTNVLYELA QYASEPTEHE QLRKMASSSG EGKELYLRWV
     LEARRHILAI LQDYPSLRPP IDHLCELLPR LQARYYSIAS SSKVHPNSVH ICAVAVEYET
     KTGRINKGVA TSWLRAKEPA GENGGRALVP MYVRKSQFRL PFKATTPVIM VGPGTGVAPF
     IGFIQERAWL RQQGKEVGET LLYYGCRRSD EDYLYREELA GFHKDGALTQ LNVAFSREQP
     QKVYVQHLLK KDKEHLWKLI HEGGAHIYVC GDARNMARDV QNTFYDIVAE QGAMEHAQAV
     DYVKKLMTKG RYSLDVWS
//