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Protein

NADPH--cytochrome P450 reductase

Gene

POR

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5 (By similarity).By similarity

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarity
  • FMNBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi170 – 20132FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi314 – 32512FADBy similarityAdd
BLAST
Nucleotide bindingi451 – 46111FADBy similarityAdd
BLAST
Nucleotide bindingi529 – 54719NADPBy similarityAdd
BLAST
Nucleotide bindingi624 – 64017NADPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductase (EC:1.6.2.4)
Short name:
CPR
Short name:
P450R
Gene namesi
Name:POR
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 678677NADPH--cytochrome P450 reductasePRO_0000245573Add
BLAST

Proteomic databases

PaxDbiQ3SYT8.
PRIDEiQ3SYT8.

Expressioni

Gene expression databases

ExpressionAtlasiQ3SYT8. baseline.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022718.

Structurei

3D structure databases

ProteinModelPortaliQ3SYT8.
SMRiQ3SYT8. Positions 64-678.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 224145Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini279 – 521243FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0369.
HOVERGENiHBG000432.
InParanoidiQ3SYT8.
KOiK00327.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SYT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSNMDAGT TTSEMVAEEV SLFSTTDVIL FSLIVGVMTY WFLFRKKKEE
60 70 80 90 100
VPEFTKIQTT TSSVKDRSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK
110 120 130 140 150
DAHRYGMRGM AADPEEYDLA DLSSLPEIEK ALAIFCMATY GEGDPTDNAQ
160 170 180 190 200
DFYDWLQETD VDLSGVKYAV FALGNKTYEH FNAMGKYVDK RLEQLGAQRI
210 220 230 240 250
FDLGLGDDDG NLEEDFITWR EQFWPAVCEH FGVEATGEES SIRQYELMVH
260 270 280 290 300
TDMDMAKVYT GEMGRLKSYE NQKPPFDAKN PFLAVVTTNR KLNQGTERHL
310 320 330 340 350
MHLELDISDS KIRYESGDHV AVYPANDSAL VNQLGEILGA DLDIIMSLNN
360 370 380 390 400
LDEESNKKHP FPCPTSYRTA LTYYLDITNP PRTNVLYELA QYASEPTEHE
410 420 430 440 450
QLRKMASSSG EGKELYLRWV LEARRHILAI LQDYPSLRPP IDHLCELLPR
460 470 480 490 500
LQARYYSIAS SSKVHPNSVH ICAVAVEYET KTGRINKGVA TSWLRAKEPA
510 520 530 540 550
GENGGRALVP MYVRKSQFRL PFKATTPVIM VGPGTGVAPF IGFIQERAWL
560 570 580 590 600
RQQGKEVGET LLYYGCRRSD EDYLYREELA GFHKDGALTQ LNVAFSREQP
610 620 630 640 650
QKVYVQHLLK KDKEHLWKLI HEGGAHIYVC GDARNMARDV QNTFYDIVAE
660 670
QGAMEHAQAV DYVKKLMTKG RYSLDVWS
Length:678
Mass (Da):77,021
Last modified:January 23, 2007 - v3
Checksum:iC4103BC020D8842C
GO

Sequence cautioni

The sequence AAI03400.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC103399 mRNA. Translation: AAI03400.1. Different initiation.
RefSeqiNP_001030467.1. NM_001035390.1.
UniGeneiBt.41220.

Genome annotation databases

GeneIDi532512.
KEGGibta:532512.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC103399 mRNA. Translation: AAI03400.1. Different initiation.
RefSeqiNP_001030467.1. NM_001035390.1.
UniGeneiBt.41220.

3D structure databases

ProteinModelPortaliQ3SYT8.
SMRiQ3SYT8. Positions 64-678.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022718.

Proteomic databases

PaxDbiQ3SYT8.
PRIDEiQ3SYT8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi532512.
KEGGibta:532512.

Organism-specific databases

CTDi5447.

Phylogenomic databases

eggNOGiCOG0369.
HOVERGENiHBG000432.
InParanoidiQ3SYT8.
KOiK00327.

Miscellaneous databases

NextBioi20875716.

Gene expression databases

ExpressionAtlasiQ3SYT8. baseline.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiNCPR_BOVIN
AccessioniPrimary (citable) accession number: Q3SYT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 77 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.