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Q3SYT8 (NCPR_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADPH--cytochrome P450 reductase

Short name=CPR
Short name=P450R
EC=1.6.2.4
Gene names
Name:POR
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length678 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5 By similarity.

Catalytic activity

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactor

FAD By similarity.

FMN By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Anchored to the ER membrane by its N-terminal hydrophobic region By similarity.

Sequence similarities

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Sequence caution

The sequence AAI03400.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 678677NADPH--cytochrome P450 reductase
PRO_0000245573

Regions

Domain80 – 224145Flavodoxin-like
Domain279 – 521243FAD-binding FR-type
Nucleotide binding170 – 20132FMN By similarity
Nucleotide binding314 – 32512FAD By similarity
Nucleotide binding451 – 46111FAD By similarity
Nucleotide binding529 – 54719NADP By similarity
Nucleotide binding624 – 64017NADP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3SYT8 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: C4103BC020D8842C

FASTA67877,021
        10         20         30         40         50         60 
MADSNMDAGT TTSEMVAEEV SLFSTTDVIL FSLIVGVMTY WFLFRKKKEE VPEFTKIQTT 

        70         80         90        100        110        120 
TSSVKDRSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK DAHRYGMRGM AADPEEYDLA 

       130        140        150        160        170        180 
DLSSLPEIEK ALAIFCMATY GEGDPTDNAQ DFYDWLQETD VDLSGVKYAV FALGNKTYEH 

       190        200        210        220        230        240 
FNAMGKYVDK RLEQLGAQRI FDLGLGDDDG NLEEDFITWR EQFWPAVCEH FGVEATGEES 

       250        260        270        280        290        300 
SIRQYELMVH TDMDMAKVYT GEMGRLKSYE NQKPPFDAKN PFLAVVTTNR KLNQGTERHL 

       310        320        330        340        350        360 
MHLELDISDS KIRYESGDHV AVYPANDSAL VNQLGEILGA DLDIIMSLNN LDEESNKKHP 

       370        380        390        400        410        420 
FPCPTSYRTA LTYYLDITNP PRTNVLYELA QYASEPTEHE QLRKMASSSG EGKELYLRWV 

       430        440        450        460        470        480 
LEARRHILAI LQDYPSLRPP IDHLCELLPR LQARYYSIAS SSKVHPNSVH ICAVAVEYET 

       490        500        510        520        530        540 
KTGRINKGVA TSWLRAKEPA GENGGRALVP MYVRKSQFRL PFKATTPVIM VGPGTGVAPF 

       550        560        570        580        590        600 
IGFIQERAWL RQQGKEVGET LLYYGCRRSD EDYLYREELA GFHKDGALTQ LNVAFSREQP 

       610        620        630        640        650        660 
QKVYVQHLLK KDKEHLWKLI HEGGAHIYVC GDARNMARDV QNTFYDIVAE QGAMEHAQAV 

       670 
DYVKKLMTKG RYSLDVWS 

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Ileum.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC103399 mRNA. Translation: AAI03400.1. Different initiation.
RefSeqNP_001030467.1. NM_001035390.1.
UniGeneBt.41220.

3D structure databases

ProteinModelPortalQ3SYT8.
SMRQ3SYT8. Positions 64-678.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ3SYT8.
PRIDEQ3SYT8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID532512.
KEGGbta:532512.

Organism-specific databases

CTD5447.

Phylogenomic databases

eggNOGCOG0369.
HOVERGENHBG000432.
InParanoidQ3SYT8.
KOK00327.

Family and domain databases

Gene3D1.20.990.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFPIRSF000208. P450R. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20875716.

Entry information

Entry nameNCPR_BOVIN
AccessionPrimary (citable) accession number: Q3SYT8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 70 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families