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Protein

Calmegin

Gene

CLGN

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions (By similarity).By similarity

GO - Molecular functioni

  1. calcium ion binding Source: Ensembl

GO - Biological processi

  1. binding of sperm to zona pellucida Source: Ensembl
  2. protein complex assembly Source: Ensembl
  3. protein folding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Calmegin
Gene namesi
Name:CLGN
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 17

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 466447LumenalSequence AnalysisAdd
BLAST
Transmembranei467 – 48721HelicalSequence AnalysisAdd
BLAST
Topological domaini488 – 606119CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. nuclear envelope Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 606587CalmeginPRO_0000236245Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi147 ↔ 181By similarity
Disulfide bondi347 ↔ 351By similarity
Modified residuei556 – 5561PhosphoserineBy similarity
Modified residuei572 – 5721PhosphoserineBy similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PRIDEiQ3SYT6.

Expressioni

Gene expression databases

ExpressionAtlasiQ3SYT6. baseline.

Interactioni

Subunit structurei

Interacts with PPIB and PDILT. Interacts with ADAM2 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ3SYT6.
SMRiQ3SYT6. Positions 47-442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati263 – 276141-1Add
BLAST
Repeati280 – 293141-2Add
BLAST
Repeati299 – 312141-3Add
BLAST
Repeati318 – 331141-4Add
BLAST
Repeati335 – 348142-1Add
BLAST
Repeati352 – 365142-2Add
BLAST
Repeati366 – 379142-3Add
BLAST
Repeati380 – 393142-4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni313 – 34634Interaction with PPIBBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG305105.
GeneTreeiENSGT00430000030841.
HOVERGENiHBG005407.
InParanoidiQ3SYT6.
KOiK09551.
OMAiEEKHAKP.
OrthoDBiEOG77126Z.
TreeFamiTF300618.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 2 hits.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SYT6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRFQGFWLCL GLLFISVNAE FMDDSVEMED FDENSEETDE LSSEIKYKTP
60 70 80 90 100
QPVGEVYFTE TFDSGRLAGW VLSKAKKDDI DAEISIYDGR WEIEELKENR
110 120 130 140 150
IPGDRGLVLK SRAKHHAISA VLAKPFIFAD KPLVVQYEVN FQDGIDCGGA
160 170 180 190 200
YIKLLADTDG LNLENFYDKT SYTIMFGPDK CGEDYKLHFI FRHKHPKTGV
210 220 230 240 250
FEEKHAKPPD VDLKRFFTDR KTHLYTLVMN PDDTFEVLID QIVVNKGSLL
260 270 280 290 300
EDVVPPINPP KEIEDPTDEK PDDWDERAKI PDASAVKPED WDESEPPQIV
310 320 330 340 350
DSSAVKPDGW LDNEPEFIPD PNAEKPFDWN EDMDGEWEAP HISNPACRIG
360 370 380 390 400
CGEWSPPMID NPKYKGIWRP PMIDNPNYQG IWSPRKIPNP DYFEDNHPFL
410 420 430 440 450
LTSFRALGLE LWSMTSDIYF DNFIICSEKE VADRWAADGW GMKILIENAN
460 470 480 490 500
EPSIFKQLMS ATEQRPWLWF IYLLTAALPI ALIGSFCWPR KVKKKYEDVA
510 520 530 540 550
FEKLDICKPQ TKGALEQEVK EEKAALEKPV DLEEEKKQSD GEIVEKEEEG
560 570 580 590 600
EPEEKSEEEI EIIEGQEEGN KSNKSGSEDE MKEADESTGS GDGPIKSVRK

RRVRKE
Length:606
Mass (Da):69,515
Last modified:October 11, 2005 - v1
Checksum:i4FAE7CE2EBF8EB4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC103401 mRNA. Translation: AAI03402.1.
RefSeqiNP_001029377.1. NM_001034205.2.
UniGeneiBt.17314.

Genome annotation databases

EnsembliENSBTAT00000002071; ENSBTAP00000002071; ENSBTAG00000001580.
GeneIDi504256.
KEGGibta:504256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC103401 mRNA. Translation: AAI03402.1.
RefSeqiNP_001029377.1. NM_001034205.2.
UniGeneiBt.17314.

3D structure databases

ProteinModelPortaliQ3SYT6.
SMRiQ3SYT6. Positions 47-442.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ3SYT6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000002071; ENSBTAP00000002071; ENSBTAG00000001580.
GeneIDi504256.
KEGGibta:504256.

Organism-specific databases

CTDi1047.

Phylogenomic databases

eggNOGiNOG305105.
GeneTreeiENSGT00430000030841.
HOVERGENiHBG005407.
InParanoidiQ3SYT6.
KOiK09551.
OMAiEEKHAKP.
OrthoDBiEOG77126Z.
TreeFamiTF300618.

Miscellaneous databases

NextBioi20866586.

Gene expression databases

ExpressionAtlasiQ3SYT6. baseline.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 2 hits.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiCLGN_BOVIN
AccessioniPrimary (citable) accession number: Q3SYT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: October 11, 2005
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.