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Protein

2-acylglycerol O-acyltransferase 2

Gene

MOGAT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity.1 Publication

Catalytic activityi

Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol.

Enzyme regulationi

Inhibited by oleic acid and sphingosine, while it is stimulated by phosphatidylcholine, phosphatidylserine and phosphatidic acid.By similarity

Kineticsi

  1. KM=45 µM for sn-1-monooleoylglycerol1 Publication

Pathwayi

GO - Molecular functioni

  1. 2-acylglycerol O-acyltransferase activity Source: UniProtKB-EC
  2. acetyltransferase activity Source: Ensembl

GO - Biological processi

  1. diacylglycerol biosynthetic process Source: Ensembl
  2. glycerol metabolic process Source: UniProtKB-KW
  3. intestinal absorption Source: Ensembl
  4. triglyceride biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BRENDAi2.3.1.22. 2681.
UniPathwayiUPA00282.

Names & Taxonomyi

Protein namesi
Recommended name:
2-acylglycerol O-acyltransferase 2 (EC:2.3.1.22)
Alternative name(s):
Acyl-CoA:monoacylglycerol acyltransferase 2
Short name:
MGAT2
Short name:
hMGAT2
Diacylglycerol O-acyltransferase candidate 5
Short name:
hDC5
Diacylglycerol acyltransferase 2-like protein 5
Monoacylglycerol O-acyltransferase 2
Gene namesi
Name:MOGAT2
Synonyms:DC5, DGAT2L5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:23248. MOGAT2.

Subcellular locationi

  1. Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei23 – 3715HelicalSequence AnalysisAdd
BLAST
Transmembranei38 – 5922HelicalSequence AnalysisAdd
BLAST
Transmembranei103 – 12321HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: MGI
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134936582.

Polymorphism and mutation databases

BioMutaiMOGAT2.
DMDMi114150036.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3343342-acylglycerol O-acyltransferase 2PRO_0000249062Add
BLAST

Proteomic databases

PaxDbiQ3SYC2.
PRIDEiQ3SYC2.

Expressioni

Tissue specificityi

Highly expressed in liver, small intestine, colon, stomach and kidney.2 Publications

Gene expression databases

BgeeiQ3SYC2.
CleanExiHS_MOGAT2.
GenevestigatoriQ3SYC2.

Organism-specific databases

HPAiHPA028834.

Interactioni

Protein-protein interaction databases

BioGridi123154. 4 interactions.
STRINGi9606.ENSP00000198801.

Structurei

3D structure databases

ProteinModelPortaliQ3SYC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG258143.
GeneTreeiENSGT00750000117391.
HOGENOMiHOG000179738.
HOVERGENiHBG065791.
InParanoidiQ3SYC2.
KOiK14457.
OMAiCKLFEEH.
OrthoDBiEOG7KH9KB.
PhylomeDBiQ3SYC2.
TreeFamiTF314707.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PANTHERiPTHR12317. PTHR12317. 1 hit.
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3SYC2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVEFAPLFMP WERRLQTLAV LQFVFSFLAL AEICTVGFIA LLFTRFWLLT
60 70 80 90 100
VLYAAWWYLD RDKPRQGGRH IQAIRCWTIW KYMKDYFPIS LVKTAELDPS
110 120 130 140 150
RNYIAGFHPH GVLAVGAFAN LCTESTGFSS IFPGIRPHLM MLTLWFRAPF
160 170 180 190 200
FRDYIMSAGL VTSEKESAAH ILNRKGGGNL LGIIVGGAQE ALDARPGSFT
210 220 230 240 250
LLLRNRKGFV RLALTHGAPL VPIFSFGEND LFDQIPNSSG SWLRYIQNRL
260 270 280 290 300
QKIMGISLPL FHGRGVFQYS FGLIPYRRPI TTVVGKPIEV QKTLHPSEEE
310 320 330
VNQLHQRYIK ELCNLFEAHK LKFNIPADQH LEFC
Length:334
Mass (Da):38,196
Last modified:September 5, 2006 - v2
Checksum:i57C4A964BAFA76BD
GO
Isoform 2 (identifier: Q3SYC2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Note: No experimental confirmation available.

Show »
Length:252
Mass (Da):28,283
Checksum:i902B6B67B2E8CC02
GO
Isoform 3 (identifier: Q3SYC2-3) [UniParc]FASTAAdd to basket

Also known as: MGAT2V, Trunc

The sequence of this isoform differs from the canonical sequence as follows:
     218-284: APLVPIFSFG...PYRRPITTVV → YQASGKSTLG...PQRRLSDLRN
     285-334: Missing.

Show »
Length:284
Mass (Da):32,298
Checksum:iE56F74332AF8D2DE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti142 – 1421L → P in BAB15436 (PubMed:14702039).Curated
Sequence conflicti267 – 2671F → L in BAC87534 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti9 – 91M → V.1 Publication
Corresponds to variant rs554202 [ dbSNP | Ensembl ].
VAR_027390
Natural varianti196 – 1961P → H.
Corresponds to variant rs34582952 [ dbSNP | Ensembl ].
VAR_048857
Natural varianti313 – 3131C → Y.
Corresponds to variant rs12281468 [ dbSNP | Ensembl ].
VAR_027391

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8282Missing in isoform 2. 1 PublicationVSP_020358Add
BLAST
Alternative sequencei218 – 28467APLVP…ITTVV → YQASGKSTLGSVGNWQGFYF GGKMAETNADSILVEIFSPF TIKIIFWCLMPKYLEKFPQR RLSDLRN in isoform 3. 1 PublicationVSP_020359Add
BLAST
Alternative sequencei285 – 33450Missing in isoform 3. 1 PublicationVSP_020360Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY157608 mRNA. Translation: AAO23672.1.
AK026297 mRNA. Translation: BAB15436.1.
AK128620 mRNA. Translation: BAC87534.1.
AK291998 mRNA. Translation: BAF84687.1.
CH471076 Genomic DNA. Translation: EAW74983.1.
BC103876 mRNA. Translation: AAI03877.1.
BC103877 mRNA. Translation: AAI03878.1.
BC103878 mRNA. Translation: AAI03879.1.
CCDSiCCDS8240.1. [Q3SYC2-1]
RefSeqiNP_079374.2. NM_025098.2. [Q3SYC2-1]
UniGeneiHs.288568.

Genome annotation databases

EnsembliENST00000198801; ENSP00000198801; ENSG00000166391. [Q3SYC2-1]
ENST00000525093; ENSP00000436537; ENSG00000166391. [Q3SYC2-3]
ENST00000526712; ENSP00000436283; ENSG00000166391. [Q3SYC2-2]
GeneIDi80168.
KEGGihsa:80168.
UCSCiuc001oww.1. human. [Q3SYC2-3]
uc010rru.2. human. [Q3SYC2-1]

Polymorphism and mutation databases

BioMutaiMOGAT2.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY157608 mRNA. Translation: AAO23672.1.
AK026297 mRNA. Translation: BAB15436.1.
AK128620 mRNA. Translation: BAC87534.1.
AK291998 mRNA. Translation: BAF84687.1.
CH471076 Genomic DNA. Translation: EAW74983.1.
BC103876 mRNA. Translation: AAI03877.1.
BC103877 mRNA. Translation: AAI03878.1.
BC103878 mRNA. Translation: AAI03879.1.
CCDSiCCDS8240.1. [Q3SYC2-1]
RefSeqiNP_079374.2. NM_025098.2. [Q3SYC2-1]
UniGeneiHs.288568.

3D structure databases

ProteinModelPortaliQ3SYC2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123154. 4 interactions.
STRINGi9606.ENSP00000198801.

Chemistry

BindingDBiQ3SYC2.
ChEMBLiCHEMBL2439944.

Polymorphism and mutation databases

BioMutaiMOGAT2.
DMDMi114150036.

Proteomic databases

PaxDbiQ3SYC2.
PRIDEiQ3SYC2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000198801; ENSP00000198801; ENSG00000166391. [Q3SYC2-1]
ENST00000525093; ENSP00000436537; ENSG00000166391. [Q3SYC2-3]
ENST00000526712; ENSP00000436283; ENSG00000166391. [Q3SYC2-2]
GeneIDi80168.
KEGGihsa:80168.
UCSCiuc001oww.1. human. [Q3SYC2-3]
uc010rru.2. human. [Q3SYC2-1]

Organism-specific databases

CTDi80168.
GeneCardsiGC11P075428.
HGNCiHGNC:23248. MOGAT2.
HPAiHPA028834.
MIMi610270. gene.
neXtProtiNX_Q3SYC2.
PharmGKBiPA134936582.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG258143.
GeneTreeiENSGT00750000117391.
HOGENOMiHOG000179738.
HOVERGENiHBG065791.
InParanoidiQ3SYC2.
KOiK14457.
OMAiCKLFEEH.
OrthoDBiEOG7KH9KB.
PhylomeDBiQ3SYC2.
TreeFamiTF314707.

Enzyme and pathway databases

UniPathwayiUPA00282.
BRENDAi2.3.1.22. 2681.

Miscellaneous databases

ChiTaRSiMOGAT2. human.
GeneWikiiMOGAT2.
GenomeRNAii80168.
NextBioi70479.
PROiQ3SYC2.
SOURCEiSearch...

Gene expression databases

BgeeiQ3SYC2.
CleanExiHS_MOGAT2.
GenevestigatoriQ3SYC2.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PANTHERiPTHR12317. PTHR12317. 1 hit.
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MGAT2, a monoacylglycerol acyltransferase expressed in the small intestine."
    Yen C.-L.E., Farese R.V. Jr.
    J. Biol. Chem. 278:18532-18537(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING (ISOFORM 3), FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    Tissue: Intestine.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Small intestine and Trachea.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT VAL-9.
  5. "Human intestinal monoacylglycerol acyltransferase: differential features in tissue expression and activity."
    Lockwood J.F., Cao J., Burn P., Shi Y.
    Am. J. Physiol. 285:E927-E937(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMOGT2_HUMAN
AccessioniPrimary (citable) accession number: Q3SYC2
Secondary accession number(s): A8K7I3
, Q3SYC1, Q6ZQZ2, Q86UH6, Q9H630
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: April 29, 2015
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.