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Q3SYC2 (MOGT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-acylglycerol O-acyltransferase 2

EC=2.3.1.22
Alternative name(s):
Acyl-CoA:monoacylglycerol acyltransferase 2
Short name=MGAT2
Short name=hMGAT2
Diacylglycerol O-acyltransferase candidate 5
Short name=hDC5
Diacylglycerol acyltransferase 2-like protein 5
Monoacylglycerol O-acyltransferase 2
Gene names
Name:MOGAT2
Synonyms:DC5, DGAT2L5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity. Ref.1

Catalytic activity

Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol.

Enzyme regulation

Inhibited by oleic acid and sphingosine, while it is stimulated by phosphatidylcholine, phosphatidylserine and phosphatidic acid By similarity.

Pathway

Glycerolipid metabolism; triacylglycerol biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1.

Tissue specificity

Highly expressed in liver, small intestine, colon, stomach and kidney. Ref.1 Ref.5

Sequence similarities

Belongs to the diacylglycerol acyltransferase family.

Biophysicochemical properties

Kinetic parameters:

KM=45 µM for sn-1-monooleoylglycerol Ref.1

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3SYC2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3SYC2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q3SYC2-3)

Also known as: MGAT2V; Trunc;

The sequence of this isoform differs from the canonical sequence as follows:
     218-284: APLVPIFSFG...PYRRPITTVV → YQASGKSTLG...PQRRLSDLRN
     285-334: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3343342-acylglycerol O-acyltransferase 2
PRO_0000249062

Regions

Transmembrane23 – 3715Helical; Potential
Transmembrane38 – 5922Helical; Potential
Transmembrane103 – 12321Helical; Potential

Natural variations

Alternative sequence1 – 8282Missing in isoform 2.
VSP_020358
Alternative sequence218 – 28467APLVP…ITTVV → YQASGKSTLGSVGNWQGFYF GGKMAETNADSILVEIFSPF TIKIIFWCLMPKYLEKFPQR RLSDLRN in isoform 3.
VSP_020359
Alternative sequence285 – 33450Missing in isoform 3.
VSP_020360
Natural variant91M → V. Ref.4
Corresponds to variant rs554202 [ dbSNP | Ensembl ].
VAR_027390
Natural variant1961P → H.
Corresponds to variant rs34582952 [ dbSNP | Ensembl ].
VAR_048857
Natural variant3131C → Y.
Corresponds to variant rs12281468 [ dbSNP | Ensembl ].
VAR_027391

Experimental info

Sequence conflict1421L → P in BAB15436. Ref.2
Sequence conflict2671F → L in BAC87534. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 5, 2006. Version 2.
Checksum: 57C4A964BAFA76BD

FASTA33438,196
        10         20         30         40         50         60 
MVEFAPLFMP WERRLQTLAV LQFVFSFLAL AEICTVGFIA LLFTRFWLLT VLYAAWWYLD 

        70         80         90        100        110        120 
RDKPRQGGRH IQAIRCWTIW KYMKDYFPIS LVKTAELDPS RNYIAGFHPH GVLAVGAFAN 

       130        140        150        160        170        180 
LCTESTGFSS IFPGIRPHLM MLTLWFRAPF FRDYIMSAGL VTSEKESAAH ILNRKGGGNL 

       190        200        210        220        230        240 
LGIIVGGAQE ALDARPGSFT LLLRNRKGFV RLALTHGAPL VPIFSFGEND LFDQIPNSSG 

       250        260        270        280        290        300 
SWLRYIQNRL QKIMGISLPL FHGRGVFQYS FGLIPYRRPI TTVVGKPIEV QKTLHPSEEE 

       310        320        330 
VNQLHQRYIK ELCNLFEAHK LKFNIPADQH LEFC 

« Hide

Isoform 2 [UniParc].

Checksum: 902B6B67B2E8CC02
Show »

FASTA25228,283
Isoform 3 (MGAT2V) (Trunc) [UniParc].

Checksum: E56F74332AF8D2DE
Show »

FASTA28432,298

References

« Hide 'large scale' references
[1]"MGAT2, a monoacylglycerol acyltransferase expressed in the small intestine."
Yen C.-L.E., Farese R.V. Jr.
J. Biol. Chem. 278:18532-18537(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING (ISOFORM 3), FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
Tissue: Intestine.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Small intestine and Trachea.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT VAL-9.
[5]"Human intestinal monoacylglycerol acyltransferase: differential features in tissue expression and activity."
Lockwood J.F., Cao J., Burn P., Shi Y.
Am. J. Physiol. 285:E927-E937(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY157608 mRNA. Translation: AAO23672.1.
AK026297 mRNA. Translation: BAB15436.1.
AK128620 mRNA. Translation: BAC87534.1.
AK291998 mRNA. Translation: BAF84687.1.
CH471076 Genomic DNA. Translation: EAW74983.1.
BC103876 mRNA. Translation: AAI03877.1.
BC103877 mRNA. Translation: AAI03878.1.
BC103878 mRNA. Translation: AAI03879.1.
CCDSCCDS8240.1. [Q3SYC2-1]
RefSeqNP_079374.2. NM_025098.2. [Q3SYC2-1]
UniGeneHs.288568.

3D structure databases

ProteinModelPortalQ3SYC2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123154. 3 interactions.
STRING9606.ENSP00000198801.

Chemistry

ChEMBLCHEMBL2439944.

Polymorphism databases

DMDM114150036.

Proteomic databases

PaxDbQ3SYC2.
PRIDEQ3SYC2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000198801; ENSP00000198801; ENSG00000166391. [Q3SYC2-1]
ENST00000525093; ENSP00000436537; ENSG00000166391. [Q3SYC2-3]
ENST00000526712; ENSP00000436283; ENSG00000166391. [Q3SYC2-2]
GeneID80168.
KEGGhsa:80168.
UCSCuc001oww.1. human. [Q3SYC2-3]
uc010rru.2. human. [Q3SYC2-1]

Organism-specific databases

CTD80168.
GeneCardsGC11P075428.
HGNCHGNC:23248. MOGAT2.
HPAHPA028834.
MIM610270. gene.
neXtProtNX_Q3SYC2.
PharmGKBPA134936582.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG258143.
HOGENOMHOG000179738.
HOVERGENHBG065791.
InParanoidQ3SYC2.
KOK14457.
OMACKLFEEH.
OrthoDBEOG7KH9KB.
PhylomeDBQ3SYC2.
TreeFamTF314707.

Enzyme and pathway databases

UniPathwayUPA00282.

Gene expression databases

BgeeQ3SYC2.
CleanExHS_MOGAT2.
GenevestigatorQ3SYC2.

Family and domain databases

InterProIPR007130. DAGAT.
[Graphical view]
PANTHERPTHR12317. PTHR12317. 1 hit.
PfamPF03982. DAGAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMOGAT2.
GenomeRNAi80168.
NextBio70479.
PROQ3SYC2.
SOURCESearch...

Entry information

Entry nameMOGT2_HUMAN
AccessionPrimary (citable) accession number: Q3SYC2
Secondary accession number(s): A8K7I3 expand/collapse secondary AC list , Q3SYC1, Q6ZQZ2, Q86UH6, Q9H630
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: July 9, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM