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Protein

2-acylglycerol O-acyltransferase 2

Gene

MOGAT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity.1 Publication

Catalytic activityi

Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol.

Enzyme regulationi

Inhibited by oleic acid and sphingosine, while it is stimulated by phosphatidylcholine, phosphatidylserine and phosphatidic acid.By similarity

Kineticsi

  1. KM=45 µM for sn-1-monooleoylglycerol1 Publication

    Pathway: triacylglycerol biosynthesis

    This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.
    View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

    Enzyme and pathway databases

    BRENDAi2.3.1.22. 2681.
    UniPathwayiUPA00282.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-acylglycerol O-acyltransferase 2 (EC:2.3.1.22)
    Alternative name(s):
    Acyl-CoA:monoacylglycerol acyltransferase 2
    Short name:
    MGAT2
    Short name:
    hMGAT2
    Diacylglycerol O-acyltransferase candidate 5
    Short name:
    hDC5
    Diacylglycerol acyltransferase 2-like protein 5
    Monoacylglycerol O-acyltransferase 2
    Gene namesi
    Name:MOGAT2
    Synonyms:DC5, DGAT2L5
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640 Componenti: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:23248. MOGAT2.

    Subcellular locationi

    • Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei23 – 3715HelicalSequence AnalysisAdd
    BLAST
    Transmembranei38 – 5922HelicalSequence AnalysisAdd
    BLAST
    Transmembranei103 – 12321HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134936582.

    Polymorphism and mutation databases

    BioMutaiMOGAT2.
    DMDMi114150036.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3343342-acylglycerol O-acyltransferase 2PRO_0000249062Add
    BLAST

    Proteomic databases

    PaxDbiQ3SYC2.
    PRIDEiQ3SYC2.

    Expressioni

    Tissue specificityi

    Highly expressed in liver, small intestine, colon, stomach and kidney.2 Publications

    Gene expression databases

    BgeeiQ3SYC2.
    CleanExiHS_MOGAT2.
    GenevisibleiQ3SYC2. HS.

    Organism-specific databases

    HPAiHPA028834.

    Interactioni

    Protein-protein interaction databases

    BioGridi123154. 4 interactions.
    STRINGi9606.ENSP00000198801.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3SYC2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG258143.
    GeneTreeiENSGT00750000117391.
    HOGENOMiHOG000179738.
    HOVERGENiHBG065791.
    InParanoidiQ3SYC2.
    KOiK14457.
    OMAiCKLFEEH.
    OrthoDBiEOG7KH9KB.
    PhylomeDBiQ3SYC2.
    TreeFamiTF314707.

    Family and domain databases

    InterProiIPR007130. DAGAT.
    [Graphical view]
    PANTHERiPTHR12317. PTHR12317. 1 hit.
    PfamiPF03982. DAGAT. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q3SYC2-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MVEFAPLFMP WERRLQTLAV LQFVFSFLAL AEICTVGFIA LLFTRFWLLT
    60 70 80 90 100
    VLYAAWWYLD RDKPRQGGRH IQAIRCWTIW KYMKDYFPIS LVKTAELDPS
    110 120 130 140 150
    RNYIAGFHPH GVLAVGAFAN LCTESTGFSS IFPGIRPHLM MLTLWFRAPF
    160 170 180 190 200
    FRDYIMSAGL VTSEKESAAH ILNRKGGGNL LGIIVGGAQE ALDARPGSFT
    210 220 230 240 250
    LLLRNRKGFV RLALTHGAPL VPIFSFGEND LFDQIPNSSG SWLRYIQNRL
    260 270 280 290 300
    QKIMGISLPL FHGRGVFQYS FGLIPYRRPI TTVVGKPIEV QKTLHPSEEE
    310 320 330
    VNQLHQRYIK ELCNLFEAHK LKFNIPADQH LEFC
    Length:334
    Mass (Da):38,196
    Last modified:September 5, 2006 - v2
    Checksum:i57C4A964BAFA76BD
    GO
    Isoform 2 (identifier: Q3SYC2-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-82: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:252
    Mass (Da):28,283
    Checksum:i902B6B67B2E8CC02
    GO
    Isoform 3 (identifier: Q3SYC2-3) [UniParc]FASTAAdd to basket

    Also known as: MGAT2V, Trunc

    The sequence of this isoform differs from the canonical sequence as follows:
         218-284: APLVPIFSFG...PYRRPITTVV → YQASGKSTLG...PQRRLSDLRN
         285-334: Missing.

    Show »
    Length:284
    Mass (Da):32,298
    Checksum:iE56F74332AF8D2DE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti142 – 1421L → P in BAB15436 (PubMed:14702039).Curated
    Sequence conflicti267 – 2671F → L in BAC87534 (PubMed:14702039).Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti9 – 91M → V.1 Publication
    Corresponds to variant rs554202 [ dbSNP | Ensembl ].
    VAR_027390
    Natural varianti196 – 1961P → H.
    Corresponds to variant rs34582952 [ dbSNP | Ensembl ].
    VAR_048857
    Natural varianti313 – 3131C → Y.
    Corresponds to variant rs12281468 [ dbSNP | Ensembl ].
    VAR_027391

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 8282Missing in isoform 2. 1 PublicationVSP_020358Add
    BLAST
    Alternative sequencei218 – 28467APLVP…ITTVV → YQASGKSTLGSVGNWQGFYF GGKMAETNADSILVEIFSPF TIKIIFWCLMPKYLEKFPQR RLSDLRN in isoform 3. 1 PublicationVSP_020359Add
    BLAST
    Alternative sequencei285 – 33450Missing in isoform 3. 1 PublicationVSP_020360Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY157608 mRNA. Translation: AAO23672.1.
    AK026297 mRNA. Translation: BAB15436.1.
    AK128620 mRNA. Translation: BAC87534.1.
    AK291998 mRNA. Translation: BAF84687.1.
    CH471076 Genomic DNA. Translation: EAW74983.1.
    BC103876 mRNA. Translation: AAI03877.1.
    BC103877 mRNA. Translation: AAI03878.1.
    BC103878 mRNA. Translation: AAI03879.1.
    CCDSiCCDS8240.1. [Q3SYC2-1]
    RefSeqiNP_079374.2. NM_025098.2. [Q3SYC2-1]
    UniGeneiHs.288568.

    Genome annotation databases

    EnsembliENST00000198801; ENSP00000198801; ENSG00000166391. [Q3SYC2-1]
    ENST00000525093; ENSP00000436537; ENSG00000166391. [Q3SYC2-3]
    ENST00000526712; ENSP00000436283; ENSG00000166391. [Q3SYC2-2]
    GeneIDi80168.
    KEGGihsa:80168.
    UCSCiuc001oww.1. human. [Q3SYC2-3]
    uc010rru.2. human. [Q3SYC2-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY157608 mRNA. Translation: AAO23672.1.
    AK026297 mRNA. Translation: BAB15436.1.
    AK128620 mRNA. Translation: BAC87534.1.
    AK291998 mRNA. Translation: BAF84687.1.
    CH471076 Genomic DNA. Translation: EAW74983.1.
    BC103876 mRNA. Translation: AAI03877.1.
    BC103877 mRNA. Translation: AAI03878.1.
    BC103878 mRNA. Translation: AAI03879.1.
    CCDSiCCDS8240.1. [Q3SYC2-1]
    RefSeqiNP_079374.2. NM_025098.2. [Q3SYC2-1]
    UniGeneiHs.288568.

    3D structure databases

    ProteinModelPortaliQ3SYC2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi123154. 4 interactions.
    STRINGi9606.ENSP00000198801.

    Chemistry

    BindingDBiQ3SYC2.
    ChEMBLiCHEMBL2439944.

    Polymorphism and mutation databases

    BioMutaiMOGAT2.
    DMDMi114150036.

    Proteomic databases

    PaxDbiQ3SYC2.
    PRIDEiQ3SYC2.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000198801; ENSP00000198801; ENSG00000166391. [Q3SYC2-1]
    ENST00000525093; ENSP00000436537; ENSG00000166391. [Q3SYC2-3]
    ENST00000526712; ENSP00000436283; ENSG00000166391. [Q3SYC2-2]
    GeneIDi80168.
    KEGGihsa:80168.
    UCSCiuc001oww.1. human. [Q3SYC2-3]
    uc010rru.2. human. [Q3SYC2-1]

    Organism-specific databases

    CTDi80168.
    GeneCardsiGC11P075428.
    HGNCiHGNC:23248. MOGAT2.
    HPAiHPA028834.
    MIMi610270. gene.
    neXtProtiNX_Q3SYC2.
    PharmGKBiPA134936582.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiNOG258143.
    GeneTreeiENSGT00750000117391.
    HOGENOMiHOG000179738.
    HOVERGENiHBG065791.
    InParanoidiQ3SYC2.
    KOiK14457.
    OMAiCKLFEEH.
    OrthoDBiEOG7KH9KB.
    PhylomeDBiQ3SYC2.
    TreeFamiTF314707.

    Enzyme and pathway databases

    UniPathwayiUPA00282.
    BRENDAi2.3.1.22. 2681.

    Miscellaneous databases

    ChiTaRSiMOGAT2. human.
    GeneWikiiMOGAT2.
    GenomeRNAii80168.
    NextBioi70479.
    PROiQ3SYC2.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ3SYC2.
    CleanExiHS_MOGAT2.
    GenevisibleiQ3SYC2. HS.

    Family and domain databases

    InterProiIPR007130. DAGAT.
    [Graphical view]
    PANTHERiPTHR12317. PTHR12317. 1 hit.
    PfamiPF03982. DAGAT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "MGAT2, a monoacylglycerol acyltransferase expressed in the small intestine."
      Yen C.-L.E., Farese R.V. Jr.
      J. Biol. Chem. 278:18532-18537(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING (ISOFORM 3), FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
      Tissue: Intestine.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Small intestine and Trachea.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT VAL-9.
    5. "Human intestinal monoacylglycerol acyltransferase: differential features in tissue expression and activity."
      Lockwood J.F., Cao J., Burn P., Shi Y.
      Am. J. Physiol. 285:E927-E937(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiMOGT2_HUMAN
    AccessioniPrimary (citable) accession number: Q3SYC2
    Secondary accession number(s): A8K7I3
    , Q3SYC1, Q6ZQZ2, Q86UH6, Q9H630
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: September 5, 2006
    Last modified: June 24, 2015
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.