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Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

Gene

DDAH2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation (By similarity).By similarity

Catalytic activityi

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei171 – 1711Proton donorBy similarity
Active sitei276 – 2761NucleophileSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.5.3.18. 908.
ReactomeiR-BTA-203615. eNOS activation.

Names & Taxonomyi

Protein namesi
Recommended name:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 (EC:3.5.3.18)
Short name:
DDAH-2
Short name:
Dimethylarginine dimethylaminohydrolase 2
Alternative name(s):
DDAHII
Dimethylargininase-2
Gene namesi
Name:DDAH2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 23

Subcellular locationi

  • Cytoplasm By similarity
  • Mitochondrion By similarity

  • Note: Translocates from cytosol to mitochondrion upon IL-1beta stimulation in chondrocytes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285N(G),N(G)-dimethylarginine dimethylaminohydrolase 2PRO_0000270759Add
BLAST

Proteomic databases

PaxDbiQ3SX44.
PeptideAtlasiQ3SX44.
PRIDEiQ3SX44.

Expressioni

Gene expression databases

BgeeiENSBTAG00000013530.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017991.

Structurei

3D structure databases

ProteinModelPortaliQ3SX44.
SMRiQ3SX44. Positions 8-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDAH family.Curated

Phylogenomic databases

eggNOGiENOG410ISNS. Eukaryota.
ENOG4111YMV. LUCA.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
InParanoidiQ3SX44.
KOiK01482.
OMAiANCIYVR.
OrthoDBiEOG091G0M5S.
TreeFamiTF314737.

Family and domain databases

InterProiIPR033199. DDAH/AD.
IPR033202. DDAH2.
[Graphical view]
PANTHERiPTHR12737. PTHR12737. 1 hit.
PTHR12737:SF16. PTHR12737:SF16. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3SX44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTPGEGLGR CSHALIRGVP ESLASGEGAA AGLPALDLAK AQREHGVLGG
60 70 80 90 100
KLRQRLGLQL VELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV
110 120 130 140 150
DGVRKALQDL GLRIVEMGDE NATLDGTDVL FTGREFFVGL SKWTNHRGAE
160 170 180 190 200
IVADTFRDFA VSTVPVTSTS HLRGLCGMGG PRTVVAGSSE AAQKAVRAMA
210 220 230 240 250
VLTDHPYASL TLPDDAAADC LFLRPGQPGL PPFLLHRGGG DLPNSQEALQ
260 270 280
KLSDVTLVPV SCSELEKAGA GLSSLCLVLS TRPHN
Length:285
Mass (Da):29,780
Last modified:October 11, 2005 - v1
Checksum:i663378431BEF4152
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104509 mRNA. Translation: AAI04510.1.
RefSeqiNP_001029876.1. NM_001034704.1.
XP_005223736.1. XM_005223679.3.
UniGeneiBt.57614.

Genome annotation databases

EnsembliENSBTAT00000017991; ENSBTAP00000017991; ENSBTAG00000013530.
GeneIDi540386.
KEGGibta:540386.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104509 mRNA. Translation: AAI04510.1.
RefSeqiNP_001029876.1. NM_001034704.1.
XP_005223736.1. XM_005223679.3.
UniGeneiBt.57614.

3D structure databases

ProteinModelPortaliQ3SX44.
SMRiQ3SX44. Positions 8-280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017991.

Proteomic databases

PaxDbiQ3SX44.
PeptideAtlasiQ3SX44.
PRIDEiQ3SX44.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000017991; ENSBTAP00000017991; ENSBTAG00000013530.
GeneIDi540386.
KEGGibta:540386.

Organism-specific databases

CTDi23564.

Phylogenomic databases

eggNOGiENOG410ISNS. Eukaryota.
ENOG4111YMV. LUCA.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
InParanoidiQ3SX44.
KOiK01482.
OMAiANCIYVR.
OrthoDBiEOG091G0M5S.
TreeFamiTF314737.

Enzyme and pathway databases

BRENDAi3.5.3.18. 908.
ReactomeiR-BTA-203615. eNOS activation.

Gene expression databases

BgeeiENSBTAG00000013530.

Family and domain databases

InterProiIPR033199. DDAH/AD.
IPR033202. DDAH2.
[Graphical view]
PANTHERiPTHR12737. PTHR12737. 1 hit.
PTHR12737:SF16. PTHR12737:SF16. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDDAH2_BOVIN
AccessioniPrimary (citable) accession number: Q3SX44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: October 11, 2005
Last modified: September 7, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.