Reviewed, UniProtKB/Swiss-Prot Q3SX21 (CLK3_BOVIN)

Names and origin

Protein names

Recommended name:
    Dual specificity protein kinase CLK3
    EC=2.7.12.1
Alternative name(s):
    CDC-like kinase 3

Gene names

Name:

CLK3

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Cytoplasmic vesicle › secretory vesicle › acrosome By similarity.
Post-translational modification	Autophosphorylates on all three types of residues By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoplasmic vesicle Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	acrosomal vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Dual specificity protein kinase CLK3		PRO_0000248283
Regions
	Domain	156 – 472	317	Protein kinase
	Nucleotide binding	162 – 170	9	ATP By similarity
Sites
	Active site	283	1	Proton acceptor By similarity
	Binding site	186	1	ATP By similarity
Amino acid modifications
	Modified residue	7	1	Phosphotyrosine By similarity
	Modified residue	9	1	Phosphoserine By similarity
	Modified residue	49	1	Phosphoserine By similarity
	Modified residue	51	1	Phosphoserine By similarity
	Modified residue	67	1	Phosphoserine By similarity
	Modified residue	76	1	Phosphoserine By similarity
	Modified residue	78	1	Phosphoserine By similarity
	Modified residue	135	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SX21-1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 0BA34A089F25B697	FASTA	490	58,628
	10 20 30 40 50 60 MHHCKRYRSP EPDPYLSYRW KRRRSYSREH EGRLRYPSRR EPPPRRSRSR SHDRLPYQRR 70 80 90 100 110 120 YREHRDSDTY RCEDRSPSFG EDYYGSSRCH HRRRSREREP YRTRKHAHHC HKRRTRSCSS 130 140 150 160 170 180 ASSRSQQSSK RSSRSVEDDK EGHLVCRIGD WLQERYEIVG NLGEGTFGKV VECLDHARGK 190 200 210 220 230 240 SQVALKIIRN VGKYREAARL EINVLKKIKE KDKENKFLCV LMSDWFNFHG HMCIAFELLG 250 260 270 280 290 300 KNTFEFLKEN NFQPYPLPHV RHMAYQLCHA LRFLHENQLT HTDLKPENIL FVNSEFETLY 310 320 330 340 350 360 NEHKSCEEKS VKNTSIRVAD FGSATFDHEH HTTIVATRHY RPPEVILELG WAQPCDVWSI 370 380 390 400 410 420 GCILFEYYRG FTLFQTHENR EHLVMMEKIL GPIPSHMIHR TRKQKYFYKG GLVWDENSSD 430 440 450 460 470 480 GRYVKENCKP LKSYMLQDTL EHVQLFDLMR RMLEFDPAQR ITLAEALLHP FFAGLTPEER 490 SFHTSRNPSR

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Uterus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC104550 mRNA. Translation: AAI04551.1.
IPI	IPI00705910.
RefSeq	NP_001029420.1.
UniGene	Bt.59406
3D structure databases
HSSP	HSSP built from PDB template 2EU9 based on UniProtKB P49761.
SMR	Q3SX21. Positions 136-481.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000009609; ENSBTAP00000009609; ENSBTAG00000007304; Bos taurus. [Genome view]
GeneID	505499.
KEGG	bta:505499.
Organism-specific databases
CTD	505499.
Phylogenomic databases
eggNOG	maNOG14181.
HOVERGEN	Q3SX21.
InParanoid	Q3SX21.
PhylomeDB	Q3SX21.
Enzyme and pathway databases
BRENDA	2.7.12.1. 251.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLK3_BOVIN

Accession

Primary (citable) accession number: Q3SX21

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	October 11, 2005
Last modified:	January 19, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)