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Protein

cAMP-dependent protein kinase inhibitor alpha

Gene

PKIA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei16Important for inhibitionBy similarity1
Sitei19Important for inhibitionBy similarity1
Sitei20Important for inhibitionBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protein kinase inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase inhibitor alpha
Short name:
PKI-alpha
Gene namesi
Name:PKIA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 14

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001545312 – 76cAMP-dependent protein kinase inhibitor alphaAdd BLAST75

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ3SX13.
PRIDEiQ3SX13.

Expressioni

Gene expression databases

BgeeiENSBTAG00000008150.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010711.

Structurei

Secondary structure

176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 12Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GNJX-ray2.28I6-25[»]
2UZTX-ray2.10B6-25[»]
2UZUX-ray2.40I6-25[»]
2UZVX-ray2.5026-25[»]
2UZWX-ray2.20I6-25[»]
3E8CX-ray2.20G/H/I/J/K/L6-25[»]
3E8EX-ray2.00C/F/G/J/N/Q6-25[»]
3ZO1X-ray2.00I6-23[»]
3ZO2X-ray1.98I6-25[»]
3ZO3X-ray2.10I6-23[»]
3ZO4X-ray1.65I6-25[»]
4C33X-ray1.70I6-23[»]
4C34X-ray1.78I6-23[»]
4C35X-ray2.19I6-23[»]
4C36X-ray1.98I6-25[»]
4C37X-ray1.70I6-25[»]
4C38X-ray1.58I6-25[»]
4YXRX-ray2.00I6-25[»]
SMRiQ3SX13.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3SX13.

Family & Domainsi

Sequence similaritiesi

Belongs to the PKI family.Curated

Phylogenomic databases

eggNOGiENOG410J11W. Eukaryota.
ENOG410YT4B. LUCA.
GeneTreeiENSGT00530000064276.
HOVERGENiHBG101093.
InParanoidiQ3SX13.
KOiK15985.
OMAiCGYLLAM.
OrthoDBiEOG091G0LVC.
TreeFamiTF330809.

Family and domain databases

InterProiIPR004171. cAMP_dep_PKI.
[Graphical view]
PfamiPF02827. PKI. 1 hit.
[Graphical view]
PIRSFiPIRSF001667. PKI. 1 hit.
ProDomiPD010366. cAMP_dep_PKI. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SX13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT
60 70
EGEEDAQRNS TEQSGEAQGE AAKSES
Length:76
Mass (Da):8,016
Last modified:January 23, 2007 - v3
Checksum:iBDDBDD1810435951
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104561 mRNA. Translation: AAI04562.1.
RefSeqiNP_001032709.1. NM_001037620.1.
UniGeneiBt.53558.

Genome annotation databases

EnsembliENSBTAT00000010711; ENSBTAP00000010711; ENSBTAG00000008150.
GeneIDi613524.
KEGGibta:613524.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104561 mRNA. Translation: AAI04562.1.
RefSeqiNP_001032709.1. NM_001037620.1.
UniGeneiBt.53558.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GNJX-ray2.28I6-25[»]
2UZTX-ray2.10B6-25[»]
2UZUX-ray2.40I6-25[»]
2UZVX-ray2.5026-25[»]
2UZWX-ray2.20I6-25[»]
3E8CX-ray2.20G/H/I/J/K/L6-25[»]
3E8EX-ray2.00C/F/G/J/N/Q6-25[»]
3ZO1X-ray2.00I6-23[»]
3ZO2X-ray1.98I6-25[»]
3ZO3X-ray2.10I6-23[»]
3ZO4X-ray1.65I6-25[»]
4C33X-ray1.70I6-23[»]
4C34X-ray1.78I6-23[»]
4C35X-ray2.19I6-23[»]
4C36X-ray1.98I6-25[»]
4C37X-ray1.70I6-25[»]
4C38X-ray1.58I6-25[»]
4YXRX-ray2.00I6-25[»]
SMRiQ3SX13.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010711.

Proteomic databases

PaxDbiQ3SX13.
PRIDEiQ3SX13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000010711; ENSBTAP00000010711; ENSBTAG00000008150.
GeneIDi613524.
KEGGibta:613524.

Organism-specific databases

CTDi5569.

Phylogenomic databases

eggNOGiENOG410J11W. Eukaryota.
ENOG410YT4B. LUCA.
GeneTreeiENSGT00530000064276.
HOVERGENiHBG101093.
InParanoidiQ3SX13.
KOiK15985.
OMAiCGYLLAM.
OrthoDBiEOG091G0LVC.
TreeFamiTF330809.

Miscellaneous databases

EvolutionaryTraceiQ3SX13.

Gene expression databases

BgeeiENSBTAG00000008150.

Family and domain databases

InterProiIPR004171. cAMP_dep_PKI.
[Graphical view]
PfamiPF02827. PKI. 1 hit.
[Graphical view]
PIRSFiPIRSF001667. PKI. 1 hit.
ProDomiPD010366. cAMP_dep_PKI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiIPKA_BOVIN
AccessioniPrimary (citable) accession number: Q3SX13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 69 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site (By similarity).By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.