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Protein

cAMP-dependent protein kinase inhibitor alpha

Gene

PKIA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.By similarity

Miscellaneous

The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei16Important for inhibitionBy similarity1
Sitei19Important for inhibitionBy similarity1
Sitei20Important for inhibitionBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionProtein kinase inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase inhibitor alpha
Short name:
PKI-alpha
Gene namesi
Name:PKIA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 14

Organism-specific databases

VGNCiVGNC:32940 PKIA

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001545312 – 76cAMP-dependent protein kinase inhibitor alphaAdd BLAST75

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ3SX13
PRIDEiQ3SX13

Expressioni

Gene expression databases

BgeeiENSBTAG00000008150

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010711

Structurei

Secondary structure

176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 12Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GNJX-ray2.28I6-25[»]
2UZTX-ray2.10B6-25[»]
2UZUX-ray2.40I6-25[»]
2UZVX-ray2.5026-25[»]
2UZWX-ray2.20I6-25[»]
3E8CX-ray2.20G/H/I/J/K/L6-25[»]
3E8EX-ray2.00C/F/G/J/N/Q6-25[»]
3ZO1X-ray2.00I6-23[»]
3ZO2X-ray1.98I6-25[»]
3ZO3X-ray2.10I6-23[»]
3ZO4X-ray1.65I6-25[»]
4C33X-ray1.70I6-23[»]
4C34X-ray1.78I6-23[»]
4C35X-ray2.19I6-23[»]
4C36X-ray1.98I6-25[»]
4C37X-ray1.70I6-25[»]
4C38X-ray1.58I6-25[»]
4YXRX-ray2.00I6-25[»]
5VHBX-ray1.61B6-23[»]
5VI9X-ray1.95B6-23[»]
5VIBX-ray2.37B6-23[»]
SMRiQ3SX13
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3SX13

Family & Domainsi

Sequence similaritiesi

Belongs to the PKI family.Curated

Phylogenomic databases

eggNOGiENOG410J11W Eukaryota
ENOG410YT4B LUCA
GeneTreeiENSGT00530000064276
HOVERGENiHBG101093
InParanoidiQ3SX13
KOiK15985
OMAiCGYLLAM
OrthoDBiEOG091G0LVC
TreeFamiTF330809

Family and domain databases

InterProiView protein in InterPro
IPR004171 cAMP_dep_PKI
PANTHERiPTHR15416 PTHR15416, 1 hit
PfamiView protein in Pfam
PF02827 PKI, 1 hit
PIRSFiPIRSF001667 PKI, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD010366 cAMP_dep_PKI, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SX13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT
60 70
EGEEDAQRNS TEQSGEAQGE AAKSES
Length:76
Mass (Da):8,016
Last modified:January 23, 2007 - v3
Checksum:iBDDBDD1810435951
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104561 mRNA Translation: AAI04562.1
RefSeqiNP_001032709.1, NM_001037620.1
UniGeneiBt.53558
Bt.91632

Genome annotation databases

EnsembliENSBTAT00000010711; ENSBTAP00000010711; ENSBTAG00000008150
GeneIDi613524
KEGGibta:613524

Similar proteinsi

Entry informationi

Entry nameiIPKA_BOVIN
AccessioniPrimary (citable) accession number: Q3SX13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 75 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health