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Protein

tRNA pseudouridine(38/39) synthase

Gene

PUS3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs.By similarity

Catalytic activityi

tRNA uridine(38/39) = tRNA pseudouridine(38/39).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei118 – 1181NucleophileBy similarity
Binding sitei195 – 1951SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine(38/39) synthase (EC:5.4.99.45)
Alternative name(s):
tRNA pseudouridine synthase 3
tRNA pseudouridylate synthase 3
tRNA-uridine isomerase 3
Gene namesi
Name:PUS3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 481480tRNA pseudouridine(38/39) synthasePRO_0000265107Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei456 – 4561PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ3SX07.
PRIDEiQ3SX07.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019065.

Structurei

3D structure databases

ProteinModelPortaliQ3SX07.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2554. Eukaryota.
COG0101. LUCA.
HOGENOMiHOG000199032.
HOVERGENiHBG053769.
InParanoidiQ3SX07.
KOiK01855.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SX07-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAENDVDRIQ TEKLLKRVQE LEQEVKRLKK EQANNKDSNI RENSSGAGGK
60 70 80 90 100
PKRAFDFSAH GQRHVALKIA YLGWGYQGFA SQENTSNTIE EKLFEALTKT
110 120 130 140 150
RLVENRQTSN YHRCGRTDKG VSAFGQVISL DLRSHIPKGR DSEHFNLKNE
160 170 180 190 200
VNDVATEIRY THILNRVLPP DIRVLAWAPV ETSFSARFSC LERTYRYFFP
210 220 230 240 250
RANLDIVTMN YAAQKYVGTH DFRNLCKMDV ANGVINFQRT ILSAQVQRVG
260 270 280 290 300
QNLGEEGWQE PFQLCQFEVT GQAFLYHQVR CMMAVLFLIG QGMEKPEVID
310 320 330 340 350
ELLNIEKNPQ KPQYSMAVEF PLVLYDCKFE NIKWIYDREV QEFNVTHLQQ
360 370 380 390 400
LWANHAVKTQ MLYSMLQGLD SVALPCGTGP KMDGMIEWRN VKPSVTKQTS
410 420 430 440 450
AFVEGVKMRT YKPLMDRPKC QGLESRIQHF VRRGRIEHPH LFHEEETKAK
460 470 480
RDCSDTLEEE NTVFEKPMKR ICVDTELKSI I
Length:481
Mass (Da):55,665
Last modified:October 11, 2005 - v1
Checksum:iD2956FB95D93F178
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104569 mRNA. Translation: AAI04570.1.
RefSeqiNP_001029684.1. NM_001034512.1.
UniGeneiBt.33259.

Genome annotation databases

GeneIDi515824.
KEGGibta:515824.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104569 mRNA. Translation: AAI04570.1.
RefSeqiNP_001029684.1. NM_001034512.1.
UniGeneiBt.33259.

3D structure databases

ProteinModelPortaliQ3SX07.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019065.

Proteomic databases

PaxDbiQ3SX07.
PRIDEiQ3SX07.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi515824.
KEGGibta:515824.

Organism-specific databases

CTDi83480.

Phylogenomic databases

eggNOGiKOG2554. Eukaryota.
COG0101. LUCA.
HOGENOMiHOG000199032.
HOVERGENiHBG053769.
InParanoidiQ3SX07.
KOiK01855.

Miscellaneous databases

NextBioi20872016.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiPUS3_BOVIN
AccessioniPrimary (citable) accession number: Q3SX07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: October 11, 2005
Last modified: May 11, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.