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Protein

Syntaxin-4

Gene

STX4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. May also play a role in docking of synaptic vesicles at presynaptic active zones (By similarity).By similarity

GO - Molecular functioni

  1. SNAP receptor activity Source: GO_Central
  2. SNARE binding Source: GO_Central

GO - Biological processi

  1. intracellular protein transport Source: GO_Central
  2. SNARE complex assembly Source: Ensembl
  3. synaptic vesicle fusion to presynaptic membrane Source: GO_Central
  4. vesicle docking Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiREACT_284336. Translocation of GLUT4 to the plasma membrane.
REACT_289964. Disinhibition of SNARE formation.
REACT_306892. Clathrin derived vesicle budding.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-4
Gene namesi
Name:STX4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 25

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 275275CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei276 – 29621Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST
Topological domaini297 – 2971ExtracellularSequence Analysis

GO - Cellular componenti

  1. basolateral plasma membrane Source: Ensembl
  2. caveola Source: AgBase
  3. cell surface Source: Ensembl
  4. endomembrane system Source: GO_Central
  5. extracellular vesicular exosome Source: Ensembl
  6. integral component of membrane Source: GO_Central
  7. lateral loop Source: Ensembl
  8. myelin sheath adaxonal region Source: Ensembl
  9. perinuclear region of cytoplasm Source: Ensembl
  10. SNARE complex Source: UniProtKB
  11. specific granule Source: Ensembl
  12. synaptic vesicle Source: GO_Central
  13. trans-Golgi network Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 297297Syntaxin-4PRO_0000282875Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei15 – 151PhosphoserineBy similarity
Modified residuei117 – 1171PhosphoserineBy similarity
Modified residuei248 – 2481PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ3SWZ3.

Interactioni

Subunit structurei

Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that interacts with SYT7 during lysosomal exocytosis. Found in a complex with VAMP8 and SNAP23. Detected in a complex with SNAP23 and STXBP4. Interacts with VAMP2. Interacts with SNAP23 and SNAPIN. Interacts with LLGL1. Interacts (via C-terminus) with CENPF. Interacts with DOC2B. Interacts with STXBP6. Interacts with STXBP3; excludes interaction with DOC2B and SNAP25. Interacts with STXBP4; excludes interaction with VAMP2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009899.

Structurei

3D structure databases

ProteinModelPortaliQ3SWZ3.
SMRiQ3SWZ3. Positions 200-255.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini200 – 26263t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili43 – 163121Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5074.
GeneTreeiENSGT00760000119200.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiQ3SWZ3.
KOiK13502.
OMAiYEMSNIN.
OrthoDBiEOG7X9G7R.
TreeFamiTF313763.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3SWZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDRTHELRQ GDDSSDDEDK ERVALVVHPG TARLGSPDDE FFQKVRTIRQ
60 70 80 90 100
TIVKLENKVR ELEKQQVTIL ATPLPEESMK QDLQNLRDEI KQLGRDIRAQ
110 120 130 140 150
LKAIEPQKEE ADENYNSVNT RMRKTQHGVL SQQFVELINK CNLMQSEYRE
160 170 180 190 200
KNVERIRRQL KITNAGMVSD KELEQMLDSG QSEVFVSNIL KDTQVTRQAL
210 220 230 240 250
NEISARHSEI QQLERSIREL HEIFTFLATE VEMQGEMINR IEKNILSSAD
260 270 280 290
YVERGQEHVK VALENQKKAR KKKVFIAICL SITVLILVVI IVISTLV
Length:297
Mass (Da):34,400
Last modified:October 11, 2005 - v1
Checksum:i5CAE09860DD5C422
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104588 mRNA. Translation: AAI04589.1.
RefSeqiNP_001030236.1. NM_001035064.1.
UniGeneiBt.3076.

Genome annotation databases

EnsembliENSBTAT00000009899; ENSBTAP00000009899; ENSBTAG00000007523.
GeneIDi508675.
KEGGibta:508675.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104588 mRNA. Translation: AAI04589.1.
RefSeqiNP_001030236.1. NM_001035064.1.
UniGeneiBt.3076.

3D structure databases

ProteinModelPortaliQ3SWZ3.
SMRiQ3SWZ3. Positions 200-255.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009899.

Proteomic databases

PRIDEiQ3SWZ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000009899; ENSBTAP00000009899; ENSBTAG00000007523.
GeneIDi508675.
KEGGibta:508675.

Organism-specific databases

CTDi6810.

Phylogenomic databases

eggNOGiCOG5074.
GeneTreeiENSGT00760000119200.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiQ3SWZ3.
KOiK13502.
OMAiYEMSNIN.
OrthoDBiEOG7X9G7R.
TreeFamiTF313763.

Enzyme and pathway databases

ReactomeiREACT_284336. Translocation of GLUT4 to the plasma membrane.
REACT_289964. Disinhibition of SNARE formation.
REACT_306892. Clathrin derived vesicle budding.

Miscellaneous databases

NextBioi20868628.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiSTX4_BOVIN
AccessioniPrimary (citable) accession number: Q3SWZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 11, 2005
Last modified: April 1, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.