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Protein

Serine/threonine-protein kinase 25

Gene

STK25

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Interaction with Golgi matrix protein GOLGA2 leads to autophosphorylation on Thr-174, possibly as a consequence of stabilization of dimer formation. The C-terminal non-catalytic region inhibits the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491ATPPROSITE-ProRule annotation
Active sitei140 – 1401Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 25 (EC:2.7.11.1)
Gene namesi
Name:STK25
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 426426Serine/threonine-protein kinase 25PRO_0000288841Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei174 – 1741Phosphothreonine; by autocatalysisBy similarity
Modified residuei278 – 2781PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3SWY6.
PRIDEiQ3SWY6.

Interactioni

Subunit structurei

Homodimer. Interacts with CTTNBP2NL.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000040234.

Structurei

3D structure databases

ProteinModelPortaliQ3SWY6.
SMRiQ3SWY6. Positions 16-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 270251Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0201. Eukaryota.
ENOG410XP9G. LUCA.
HOGENOMiHOG000234203.
HOVERGENiHBG108518.
InParanoidiQ3SWY6.
KOiK08838.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3SWY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHLRGFANQ HSRVDPEELF TKLDRIGKGS FGEVYKGIDN RTKEVVAIKI
60 70 80 90 100
IDLEEAEDEI EDIQQEITVL SQCDSPYITR YFGSYLKSTK LWIIMEYLGG
110 120 130 140 150
GSALDLLKPG PLEETYIATI LREILKGLDY LHSERKIHRD IKAANVLLSE
160 170 180 190 200
QGDVKLADFG VAGQLTDTQI KRNTFVGTPF WMAPEVIKQS AYDFKADIWS
210 220 230 240 250
LGITAIELAK GEPPNSDLHP MRVLFLIPKN SPPTLEGHHS KPFKEFVEAC
260 270 280 290 300
LNKDPRFRPT AKELLKHKFI TRYTKKTSFL TELIDRYKRW RSEGHGEESS
310 320 330 340 350
SEDSDIDGDP EDGEQGPIWT FPPTIRPSPH GKLHKGTALH GPQKSAEPVK
360 370 380 390 400
RQPRSQCLST LVRPVFGELK EKHKQSGGGV GALEELENAF SLAEESCPGI
410 420
SDKLMAHLVE RVQRFSHSRN HLTSTR
Length:426
Mass (Da):48,035
Last modified:October 11, 2005 - v1
Checksum:i436AB21D7BE7FF8E
GO

Sequence cautioni

The sequence AAQ55285 differs from that shown. Reason: Frameshift at positions 317 and 333. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti344 – 3441K → N in AAQ55285 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY346153 mRNA. Translation: AAQ55285.1. Frameshift.
BC104597 mRNA. Translation: AAI04598.1.
RefSeqiNP_899666.1. NM_183407.2.
UniGeneiBt.23261.

Genome annotation databases

GeneIDi373543.
KEGGibta:373543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY346153 mRNA. Translation: AAQ55285.1. Frameshift.
BC104597 mRNA. Translation: AAI04598.1.
RefSeqiNP_899666.1. NM_183407.2.
UniGeneiBt.23261.

3D structure databases

ProteinModelPortaliQ3SWY6.
SMRiQ3SWY6. Positions 16-293.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000040234.

Proteomic databases

PaxDbiQ3SWY6.
PRIDEiQ3SWY6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi373543.
KEGGibta:373543.

Organism-specific databases

CTDi10494.

Phylogenomic databases

eggNOGiKOG0201. Eukaryota.
ENOG410XP9G. LUCA.
HOGENOMiHOG000234203.
HOVERGENiHBG108518.
InParanoidiQ3SWY6.
KOiK08838.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTK25_BOVIN
AccessioniPrimary (citable) accession number: Q3SWY6
Secondary accession number(s): Q6V9V8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 11, 2005
Last modified: July 6, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.