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Protein

Thrombospondin-4

Gene

THBS4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Mitogen

Keywords - Biological processi

Cell adhesion, Tissue remodeling, Unfolded protein response

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-BTA-186797. Signaling by PDGF.

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-4
Gene namesi
Name:THBS4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 10

Subcellular locationi

  • Endoplasmic reticulum By similarity
  • Sarcoplasmic reticulum By similarity
  • Secreted By similarity
  • Secretedextracellular space By similarity
  • Secretedextracellular spaceextracellular matrix By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Extracellular matrix, Sarcoplasmic reticulum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000028303224 – 961Thrombospondin-4Add BLAST938

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi258InterchainCurated
Disulfide bondi261InterchainCurated
Disulfide bondi290 ↔ 301PROSITE-ProRule annotation
Disulfide bondi295 ↔ 310PROSITE-ProRule annotation
Disulfide bondi313 ↔ 324PROSITE-ProRule annotation
Disulfide bondi330 ↔ 341PROSITE-ProRule annotation
Disulfide bondi335 ↔ 350PROSITE-ProRule annotation
Disulfide bondi353 ↔ 377PROSITE-ProRule annotation
Disulfide bondi383 ↔ 394PROSITE-ProRule annotation
Disulfide bondi388 ↔ 403PROSITE-ProRule annotation
Disulfide bondi406 ↔ 418PROSITE-ProRule annotation
Disulfide bondi424 ↔ 438PROSITE-ProRule annotation
Disulfide bondi432 ↔ 448PROSITE-ProRule annotation
Disulfide bondi450 ↔ 461PROSITE-ProRule annotation
Disulfide bondi477 ↔ 482PROSITE-ProRule annotation
Disulfide bondi487 ↔ 507PROSITE-ProRule annotation
Disulfide bondi523 ↔ 543PROSITE-ProRule annotation
Disulfide bondi546 ↔ 566PROSITE-ProRule annotation
Disulfide bondi582 ↔ 602PROSITE-ProRule annotation
Disulfide bondi605 ↔ 625PROSITE-ProRule annotation
Glycosylationi612N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi643 ↔ 663PROSITE-ProRule annotation
Disulfide bondi683 ↔ 703PROSITE-ProRule annotation
Disulfide bondi719 ↔ 940PROSITE-ProRule annotation
Glycosylationi941N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ3SWW8.
PeptideAtlasiQ3SWW8.
PRIDEiQ3SWW8.

Expressioni

Gene expression databases

BgeeiENSBTAG00000012866.

Interactioni

Subunit structurei

Homopentamer; disulfide-linked. Interacts with PTBP3 (By similarity). Interacts (via EGF-like 3; calcium-binding domain) with ATF6 and facilitates its processing, activation and nuclear translocation. Interacts with NOTCH1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000036460.

Structurei

3D structure databases

ProteinModelPortaliQ3SWW8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 192Laminin G-likeAdd BLAST169
Domaini286 – 325EGF-like 1PROSITE-ProRule annotationAdd BLAST40
Domaini326 – 363EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST38
Domaini379 – 419EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini420 – 462EGF-like 4PROSITE-ProRule annotationAdd BLAST43
Repeati463 – 495TSP type-3 1Add BLAST33
Repeati496 – 531TSP type-3 2Add BLAST36
Repeati532 – 554TSP type-3 3Add BLAST23
Repeati555 – 590TSP type-3 4Add BLAST36
Repeati591 – 613TSP type-3 5Add BLAST23
Repeati614 – 651TSP type-3 6Add BLAST38
Repeati652 – 691TSP type-3 7Add BLAST40
Repeati692 – 727TSP type-3 8Add BLAST36
Domaini731 – 945TSP C-terminalPROSITE-ProRule annotationAdd BLAST215

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi562 – 564Cell attachment siteSequence analysis3

Sequence similaritiesi

Belongs to the thrombospondin family.Curated
Contains 4 EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin G-like domain.Curated
Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IFQQ. Eukaryota.
ENOG410XQKE. LUCA.
GeneTreeiENSGT00850000132273.
HOGENOMiHOG000007542.
HOVERGENiHBG000636.
InParanoidiQ3SWW8.
KOiK04659.
OMAiKGTQTYW.
OrthoDBiEOG091G00TV.
TreeFamiTF324917.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PfamiPF11598. COMP. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 4 hits.
SM00179. EGF_CA. 3 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3SWW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAPRGATFL LLHLALQPWL GAGAQATPQV FDLLPSASQR LNPSVLQPIL
60 70 80 90 100
TDPTLNELYV ISTFKLQSKS SATIFGLYSS ADHSKYFEFT VMGRLNKAIL
110 120 130 140 150
RYLKNDGRIH LVVFNNLQLA DGRRHRLLLR LTNLHRGAGS VELFLDCTRV
160 170 180 190 200
DSIHNLPRAF SGLAQSPEAV ELRTFQRKAH DSLEELKLVV RGSLIQVASL
210 220 230 240 250
QDCFLQQSEP LATTNTGDFN RQFLGQMSQL NQLLGEVKDL LRQQVKETSF
260 270 280 290 300
LRNTIAECQA CGPLSFQSPT PNTLMPVVPA ASPTPPVRRC DSNPCFRGVR
310 320 330 340 350
CTDTRDGFQC GPCPEGYTGN GIVCSDVDEC RYHPCYPGVR CVNLAPGFRC
360 370 380 390 400
DACPVGFTGP MMQGVGISFA KTNKQVCTDI DECRNGACVL NSICINTLGS
410 420 430 440 450
YRCGPCKPGY IGDQMRGCKM ERNCRDPELN PCSVNAQCIE ERQGDVTCVC
460 470 480 490 500
GVGWAGDGYI CGKDVDIDSY PDEELPCSAR NCKKDNCKYV PNSGQEDADR
510 520 530 540 550
DGIGDACDDD ADGDGILNEQ DNCVLTHNVD QRNSDKDIFG DACDNCRNVL
560 570 580 590 600
NNDQKDTDGD GKGDACDDDM DGDGIKNILD NCQKVPNSDQ EDRDGDGVGD
610 620 630 640 650
ACDSCPEVSN PNQSDVDNDL VGDSCDTNQD SDGDGHQDST DNCPTVINSA
660 670 680 690 700
QLDTDKDGIG DECDDDDDND GIPDLVPPGP DNCRLVPNPA QEDSNSDGVG
710 720 730 740 750
DICEADFDQD QVIDRIDVCP ENAEVTLTDF RAYQTVVLDP EGDAQIDPNW
760 770 780 790 800
VVLNQGMEIV QTMNSDPGLA VGYTAFNGVD FEGTFHVNTQ TDDDYAGFIF
810 820 830 840 850
GYQDSSSFYV VMWKQTEQTY WQATPFRAVA EPGIQLKAVK SKTGPGEHLR
860 870 880 890 900
NSLWHTGDTS DQVRLLWKDS RNVGWKDKVS YRWFLQHRPQ VGYIRVRFYE
910 920 930 940 950
GSELVADSGV TIDTTMRGGR LGVFCFSQEN IIWSNLKYRC NDTIPEDFQE
960
FQTQNFDRLD K
Length:961
Mass (Da):105,974
Last modified:October 11, 2005 - v1
Checksum:i5D51CA6752629A09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104630 mRNA. Translation: AAI04631.1.
RefSeqiNP_001029900.1. NM_001034728.1.
UniGeneiBt.11641.

Genome annotation databases

EnsembliENSBTAT00000036603; ENSBTAP00000036460; ENSBTAG00000012866.
GeneIDi541281.
KEGGibta:541281.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC104630 mRNA. Translation: AAI04631.1.
RefSeqiNP_001029900.1. NM_001034728.1.
UniGeneiBt.11641.

3D structure databases

ProteinModelPortaliQ3SWW8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000036460.

Proteomic databases

PaxDbiQ3SWW8.
PeptideAtlasiQ3SWW8.
PRIDEiQ3SWW8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000036603; ENSBTAP00000036460; ENSBTAG00000012866.
GeneIDi541281.
KEGGibta:541281.

Organism-specific databases

CTDi7060.

Phylogenomic databases

eggNOGiENOG410IFQQ. Eukaryota.
ENOG410XQKE. LUCA.
GeneTreeiENSGT00850000132273.
HOGENOMiHOG000007542.
HOVERGENiHBG000636.
InParanoidiQ3SWW8.
KOiK04659.
OMAiKGTQTYW.
OrthoDBiEOG091G00TV.
TreeFamiTF324917.

Enzyme and pathway databases

ReactomeiR-BTA-186797. Signaling by PDGF.

Gene expression databases

BgeeiENSBTAG00000012866.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PfamiPF11598. COMP. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 4 hits.
SM00179. EGF_CA. 3 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSP4_BOVIN
AccessioniPrimary (citable) accession number: Q3SWW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 11, 2005
Last modified: October 5, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.