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Q3SWW8 (TSP4_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thrombospondin-4
Gene names
Name:THBS4
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length961 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner By similarity.

Subunit structure

Homopentamer; disulfide-linked. Interacts with PTBP3 By similarity. Interacts (via EGF-like 3; calcium-binding domain) with ATF6 and facilitates its processing, activation and nuclear translocation. Interacts with NOTCH1 By similarity.

Subcellular location

Endoplasmic reticulum By similarity. Sarcoplasmic reticulum By similarity. Secreted By similarity. Secretedextracellular space By similarity. Secretedextracellular spaceextracellular matrix By similarity.

Sequence similarities

Belongs to the thrombospondin family.

Contains 4 EGF-like domains.

Contains 1 laminin G-like domain.

Contains 1 TSP C-terminal (TSPC) domain.

Contains 8 TSP type-3 repeats.

Ontologies

Keywords
   Biological processCell adhesion
Tissue remodeling
Unfolded protein response
   Cellular componentEndoplasmic reticulum
Extracellular matrix
Sarcoplasmic reticulum
Secreted
   DomainEGF-like domain
Repeat
Signal
   LigandCalcium
   Molecular functionGrowth factor
Mitogen
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbehavioral response to pain

Inferred from sequence or structural similarity. Source: UniProtKB

endothelial cell-cell adhesion

Inferred from electronic annotation. Source: Ensembl

myoblast migration

Inferred from electronic annotation. Source: Ensembl

negative regulation of angiogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell division

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of endothelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of neutrophil chemotaxis

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from electronic annotation. Source: Ensembl

regulation of tissue remodeling

Inferred from sequence or structural similarity. Source: UniProtKB

response to endoplasmic reticulum stress

Inferred from sequence or structural similarity. Source: UniProtKB

response to unfolded protein

Inferred from electronic annotation. Source: UniProtKB-KW

tissue remodeling

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentbasement membrane

Inferred from electronic annotation. Source: Ensembl

endoplasmic reticulum

Inferred from sequence or structural similarity. Source: UniProtKB

extracellular matrix

Inferred from sequence or structural similarity. Source: UniProtKB

extracellular space

Inferred from sequence or structural similarity. Source: UniProtKB

sarcoplasmic reticulum

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

heparin binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 961938Thrombospondin-4
PRO_0000283032

Regions

Domain24 – 192169Laminin G-like
Domain286 – 32540EGF-like 1
Domain326 – 36338EGF-like 2; calcium-binding Potential
Domain379 – 41941EGF-like 3; calcium-binding Potential
Domain420 – 46243EGF-like 4
Repeat463 – 49533TSP type-3 1
Repeat496 – 53136TSP type-3 2
Repeat532 – 55423TSP type-3 3
Repeat555 – 59036TSP type-3 4
Repeat591 – 61323TSP type-3 5
Repeat614 – 65138TSP type-3 6
Repeat652 – 69140TSP type-3 7
Repeat692 – 72736TSP type-3 8
Domain731 – 945215TSP C-terminal
Motif562 – 5643Cell attachment site Potential

Amino acid modifications

Glycosylation6121N-linked (GlcNAc...) Potential
Glycosylation9411N-linked (GlcNAc...) Potential
Disulfide bond258Interchain Probable
Disulfide bond261Interchain Probable
Disulfide bond290 ↔ 301 By similarity
Disulfide bond295 ↔ 310 By similarity
Disulfide bond313 ↔ 324 By similarity
Disulfide bond330 ↔ 341 By similarity
Disulfide bond335 ↔ 350 By similarity
Disulfide bond353 ↔ 377 By similarity
Disulfide bond383 ↔ 394 By similarity
Disulfide bond388 ↔ 403 By similarity
Disulfide bond406 ↔ 418 By similarity
Disulfide bond424 ↔ 438 By similarity
Disulfide bond432 ↔ 448 By similarity
Disulfide bond450 ↔ 461 By similarity
Disulfide bond477 ↔ 482 By similarity
Disulfide bond487 ↔ 507 By similarity
Disulfide bond523 ↔ 543 By similarity
Disulfide bond546 ↔ 566 By similarity
Disulfide bond582 ↔ 602 By similarity
Disulfide bond605 ↔ 625 By similarity
Disulfide bond643 ↔ 663 By similarity
Disulfide bond683 ↔ 703 By similarity
Disulfide bond719 ↔ 940 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3SWW8 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 5D51CA6752629A09

FASTA961105,974
        10         20         30         40         50         60 
MLAPRGATFL LLHLALQPWL GAGAQATPQV FDLLPSASQR LNPSVLQPIL TDPTLNELYV 

        70         80         90        100        110        120 
ISTFKLQSKS SATIFGLYSS ADHSKYFEFT VMGRLNKAIL RYLKNDGRIH LVVFNNLQLA 

       130        140        150        160        170        180 
DGRRHRLLLR LTNLHRGAGS VELFLDCTRV DSIHNLPRAF SGLAQSPEAV ELRTFQRKAH 

       190        200        210        220        230        240 
DSLEELKLVV RGSLIQVASL QDCFLQQSEP LATTNTGDFN RQFLGQMSQL NQLLGEVKDL 

       250        260        270        280        290        300 
LRQQVKETSF LRNTIAECQA CGPLSFQSPT PNTLMPVVPA ASPTPPVRRC DSNPCFRGVR 

       310        320        330        340        350        360 
CTDTRDGFQC GPCPEGYTGN GIVCSDVDEC RYHPCYPGVR CVNLAPGFRC DACPVGFTGP 

       370        380        390        400        410        420 
MMQGVGISFA KTNKQVCTDI DECRNGACVL NSICINTLGS YRCGPCKPGY IGDQMRGCKM 

       430        440        450        460        470        480 
ERNCRDPELN PCSVNAQCIE ERQGDVTCVC GVGWAGDGYI CGKDVDIDSY PDEELPCSAR 

       490        500        510        520        530        540 
NCKKDNCKYV PNSGQEDADR DGIGDACDDD ADGDGILNEQ DNCVLTHNVD QRNSDKDIFG 

       550        560        570        580        590        600 
DACDNCRNVL NNDQKDTDGD GKGDACDDDM DGDGIKNILD NCQKVPNSDQ EDRDGDGVGD 

       610        620        630        640        650        660 
ACDSCPEVSN PNQSDVDNDL VGDSCDTNQD SDGDGHQDST DNCPTVINSA QLDTDKDGIG 

       670        680        690        700        710        720 
DECDDDDDND GIPDLVPPGP DNCRLVPNPA QEDSNSDGVG DICEADFDQD QVIDRIDVCP 

       730        740        750        760        770        780 
ENAEVTLTDF RAYQTVVLDP EGDAQIDPNW VVLNQGMEIV QTMNSDPGLA VGYTAFNGVD 

       790        800        810        820        830        840 
FEGTFHVNTQ TDDDYAGFIF GYQDSSSFYV VMWKQTEQTY WQATPFRAVA EPGIQLKAVK 

       850        860        870        880        890        900 
SKTGPGEHLR NSLWHTGDTS DQVRLLWKDS RNVGWKDKVS YRWFLQHRPQ VGYIRVRFYE 

       910        920        930        940        950        960 
GSELVADSGV TIDTTMRGGR LGVFCFSQEN IIWSNLKYRC NDTIPEDFQE FQTQNFDRLD 


K 

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References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Ascending colon.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC104630 mRNA. Translation: AAI04631.1.
RefSeqNP_001029900.1. NM_001034728.1.
UniGeneBt.11641.

3D structure databases

ProteinModelPortalQ3SWW8.
SMRQ3SWW8. Positions 218-262, 603-942.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000036460.

Proteomic databases

PaxDbQ3SWW8.
PRIDEQ3SWW8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000036603; ENSBTAP00000036460; ENSBTAG00000012866.
GeneID541281.
KEGGbta:541281.

Organism-specific databases

CTD7060.

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00550000074507.
HOGENOMHOG000007542.
HOVERGENHBG000636.
InParanoidQ3SWW8.
KOK04659.
OMAAECQACG.
OrthoDBEOG76QFGD.
TreeFamTF324917.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
[Graphical view]
PfamPF11598. COMP. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTSM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 2 hits.
PROSITEPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20879115.

Entry information

Entry nameTSP4_BOVIN
AccessionPrimary (citable) accession number: Q3SWW8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 11, 2005
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families