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Protein

RISC-loading complex subunit TARBP2

Gene

Tarbp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

RNA-mediated gene silencing, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-203927. MicroRNA (miRNA) biogenesis.
R-RNO-426486. Small interfering RNA (siRNA) biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
RISC-loading complex subunit TARBP2UniRule annotation
Gene namesi
Name:Tarbp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1307375. Tarbp2.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Cytoplasmperinuclear region UniRule annotation
  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365RISC-loading complex subunit TARBP2PRO_0000373971Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei151 – 1511PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3SWU0.

Expressioni

Gene expression databases

GenevisibleiQ3SWU0. RN.

Interactioni

Subunit structurei

Self-associates. Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with EIF2AK2/PKR and inhibits its protein kinase activity. Interacts with DHX9 and PRKRA. Interacts with DICER1, AGO2, MOV10, EIF6 and RPL7A (60S ribosome subunit); they form a large RNA-induced silencing complex (RISC).UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061329.

Structurei

3D structure databases

ProteinModelPortaliQ3SWU0.
SMRiQ3SWU0. Positions 149-224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 9768DRBM 1UniRule annotationAdd
BLAST
Domaini158 – 22669DRBM 2UniRule annotationAdd
BLAST
Domaini292 – 36069DRBM 3UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 10584Sufficient for interaction with PRKRAUniRule annotationAdd
BLAST
Regioni151 – 23383Sufficient for interaction with PRKRAUniRule annotationAdd
BLAST
Regioni227 – 365139Sufficient for interaction with DICER1UniRule annotationAdd
BLAST
Regioni286 – 36580Sufficient for interaction with PRKRAUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TARBP2 family.UniRule annotation
Contains 3 DRBM (double-stranded RNA-binding) domains.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3732. Eukaryota.
ENOG410XSCK. LUCA.
GeneTreeiENSGT00830000128290.
HOGENOMiHOG000231919.
InParanoidiQ3SWU0.
KOiK18420.
OMAiQYLRIMA.
OrthoDBiEOG741Z2Q.
PhylomeDBiQ3SWU0.

Family and domain databases

Gene3Di3.30.160.20. 3 hits.
HAMAPiMF_03034. TRBP2.
InterProiIPR014720. dsRBD_dom.
IPR028605. TRBP2.
[Graphical view]
PANTHERiPTHR10910:SF61. PTHR10910:SF61. 1 hit.
PfamiPF00035. dsrm. 2 hits.
[Graphical view]
SMARTiSM00358. DSRM. 3 hits.
[Graphical view]
PROSITEiPS50137. DS_RBD. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3SWU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEEDQGSGS TTGCGLPSIE QMLAANPGKT PISLLQEYGT RIGKTPVYDL
60 70 80 90 100
LKAEGQAHQP NFTFRVTVGD TSCTGQGPSK KAAKHKAAEV ALKHLKGGSM
110 120 130 140 150
LEPALEDSSS FSLLDSSVPE DTAVIAAEAA APVPSALLTR SPPMEMQPPV
160 170 180 190 200
SPQQSECNPV GALQELVVQK GWRLPEYMVT QESGPAHRKE FTMTCRVERF
210 220 230 240 250
IEIGSGTSKK LAKRNAAAKM LLRVHTVPLD ARDGNEAEPD DDHFSIGVSS
260 270 280 290 300
RLDGLRNRGP GCTWDSLRNS VGEKILSLRN CSVGSLGSLG SACCSILSEL
310 320 330 340 350
SAEQAFHVSY LDIEELSLSG LCQCLVELST QPATVCYGSA TTREAARGDA
360
ARRALQYLRI MAGSK
Length:365
Mass (Da):38,850
Last modified:October 11, 2005 - v1
Checksum:i5027E6C492840EAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474035 mRNA. Translation: EDL86826.1.
BC104690 mRNA. Translation: AAI04691.1.
RefSeqiNP_001030113.1. NM_001034941.1.
UniGeneiRn.17227.

Genome annotation databases

EnsembliENSRNOT00000064661; ENSRNOP00000061329; ENSRNOG00000042355.
GeneIDi363006.
KEGGirno:363006.
UCSCiRGD:1307375. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474035 mRNA. Translation: EDL86826.1.
BC104690 mRNA. Translation: AAI04691.1.
RefSeqiNP_001030113.1. NM_001034941.1.
UniGeneiRn.17227.

3D structure databases

ProteinModelPortaliQ3SWU0.
SMRiQ3SWU0. Positions 149-224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061329.

Proteomic databases

PaxDbiQ3SWU0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000064661; ENSRNOP00000061329; ENSRNOG00000042355.
GeneIDi363006.
KEGGirno:363006.
UCSCiRGD:1307375. rat.

Organism-specific databases

CTDi6895.
RGDi1307375. Tarbp2.

Phylogenomic databases

eggNOGiKOG3732. Eukaryota.
ENOG410XSCK. LUCA.
GeneTreeiENSGT00830000128290.
HOGENOMiHOG000231919.
InParanoidiQ3SWU0.
KOiK18420.
OMAiQYLRIMA.
OrthoDBiEOG741Z2Q.
PhylomeDBiQ3SWU0.

Enzyme and pathway databases

ReactomeiR-RNO-203927. MicroRNA (miRNA) biogenesis.
R-RNO-426486. Small interfering RNA (siRNA) biogenesis.

Miscellaneous databases

PROiQ3SWU0.

Gene expression databases

GenevisibleiQ3SWU0. RN.

Family and domain databases

Gene3Di3.30.160.20. 3 hits.
HAMAPiMF_03034. TRBP2.
InterProiIPR014720. dsRBD_dom.
IPR028605. TRBP2.
[Graphical view]
PANTHERiPTHR10910:SF61. PTHR10910:SF61. 1 hit.
PfamiPF00035. dsrm. 2 hits.
[Graphical view]
SMARTiSM00358. DSRM. 3 hits.
[Graphical view]
PROSITEiPS50137. DS_RBD. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiTRBP2_RAT
AccessioniPrimary (citable) accession number: Q3SWU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: October 11, 2005
Last modified: July 6, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.