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Q3SWQ3 (TRMB_NITWN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Nwi_0020
OrganismNitrobacter winogradskyi (strain Nb-255 / ATCC 25391) [Complete proteome] [HAMAP]
Taxonomic identifier323098 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter ›

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 257257tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000229178

Sites

Binding site841S-adenosyl-L-methionine By similarity
Binding site1091S-adenosyl-L-methionine By similarity
Binding site1361S-adenosyl-L-methionine By similarity
Binding site1581S-adenosyl-L-methionine By similarity
Binding site1621Substrate By similarity
Binding site1941Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q3SWQ3 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: A37A15B209D03334

FASTA25729,020
        10         20         30         40         50         60 
MTVVVSDHQN PRPPGDDAAP LGRTGNRDRP PGSFFGRRKG HRLRPHQNDL VAQLLPRLGF 

        70         80         90        100        110        120 
DPARIGPSGP AALFDPPVDE TRIEIGFGGG EHLVAEALAF PRAGFIGCEP YVNGMAKILV 

       130        140        150        160        170        180 
QIEAHAIRNI RLFAGDASQL LACLPPASLA RIDLIHPDPW PKRRHWKRRF VQDATVAEMA 

       190        200        210        220        230        240 
RVLRPEGEFR FVSDIDDYCA WTLTHLARSP DFLWLAERSA DWQDPWSGYT MTRYGRKAMR 

       250 
EGRRAAYLRF RRERTAV

« Hide

References

[1]"Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255."
Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J.
Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nb-255 / ATCC 25391.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000115 Genomic DNA. Translation: ABA03288.1.
RefSeqYP_316640.1. NC_007406.1.

3D structure databases

ProteinModelPortalQ3SWQ3.
ModBaseSearch...

Protein-protein interaction databases

STRING323098.Nwi_0020.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA03288; ABA03288; Nwi_0020.
GeneID3676460.
KEGGnwi:Nwi_0020.
PATRIC22696333. VBINitWin102302_0023.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMALYPDPWP.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycNWIN323098:GJEG-20-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMB_NITWN
AccessionPrimary (citable) accession number: Q3SWQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 11, 2005
Last modified: May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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