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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84S-adenosyl-L-methionineUniRule annotation1
Binding sitei109S-adenosyl-L-methionineUniRule annotation1
Binding sitei136S-adenosyl-L-methionineUniRule annotation1
Active sitei158By similarity1
Binding sitei158S-adenosyl-L-methionineUniRule annotation1
Binding sitei162SubstrateUniRule annotation1
Binding sitei194SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Nwi_0020
OrganismiNitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)
Taxonomic identifieri323098 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
Proteomesi
  • UP000002531 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002291781 – 257tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST257

Interactioni

Protein-protein interaction databases

STRINGi323098.Nwi_0020.

Structurei

3D structure databases

ProteinModelPortaliQ3SWQ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073969.
KOiK03439.
OMAiWPKKKHW.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3SWQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVVVSDHQN PRPPGDDAAP LGRTGNRDRP PGSFFGRRKG HRLRPHQNDL
60 70 80 90 100
VAQLLPRLGF DPARIGPSGP AALFDPPVDE TRIEIGFGGG EHLVAEALAF
110 120 130 140 150
PRAGFIGCEP YVNGMAKILV QIEAHAIRNI RLFAGDASQL LACLPPASLA
160 170 180 190 200
RIDLIHPDPW PKRRHWKRRF VQDATVAEMA RVLRPEGEFR FVSDIDDYCA
210 220 230 240 250
WTLTHLARSP DFLWLAERSA DWQDPWSGYT MTRYGRKAMR EGRRAAYLRF

RRERTAV
Length:257
Mass (Da):29,020
Last modified:October 11, 2005 - v1
Checksum:iA37A15B209D03334
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000115 Genomic DNA. Translation: ABA03288.1.
RefSeqiWP_011313359.1. NC_007406.1.

Genome annotation databases

EnsemblBacteriaiABA03288; ABA03288; Nwi_0020.
KEGGinwi:Nwi_0020.
PATRICi22696333. VBINitWin102302_0023.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000115 Genomic DNA. Translation: ABA03288.1.
RefSeqiWP_011313359.1. NC_007406.1.

3D structure databases

ProteinModelPortaliQ3SWQ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323098.Nwi_0020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA03288; ABA03288; Nwi_0020.
KEGGinwi:Nwi_0020.
PATRICi22696333. VBINitWin102302_0023.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073969.
KOiK03439.
OMAiWPKKKHW.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_NITWN
AccessioniPrimary (citable) accession number: Q3SWQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.