Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei633 – 6331ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNWIN323098:GJEG-92-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Nwi_0091
OrganismiNitrobacter winogradskyi (strain Nb-255 / ATCC 25391)
Taxonomic identifieri323098 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
ProteomesiUP000002531 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 883883Leucine--tRNA ligasePRO_1000009383Add
BLAST

Proteomic databases

PRIDEiQ3SWI2.

Interactioni

Protein-protein interaction databases

STRINGi323098.Nwi_0091.

Structurei

3D structure databases

ProteinModelPortaliQ3SWI2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi630 – 6345"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3SWI2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSERYNARD AEPRWQAEWD RQAIFATKND DPREKYYVLE MFPYPSGRIH
60 70 80 90 100
IGHVRNYTLG DVIARYMRAR GYNVLHPMGW DAFGLPAENA AIERKIAPKA
110 120 130 140 150
WTYDNIKAMK KQLRSIGLSL DWSREIATCD PAYYKHQQKM FLDFLRAGLA
160 170 180 190 200
EREKRKINWD PVDMTVLANE QVIDGRGWRS GAVVEQREMN QWVFKITKFS
210 220 230 240 250
QELLDALGTL DRWPDKVRLM QRNWIGRSEG LLIRFALDAT TTPNNESELK
260 270 280 290 300
IFTTRPDTLF GARFMAIAPD HPLAQAAAKD NPALAGFIAE CKQRGTAQAE
310 320 330 340 350
IDTAEKMGFD TGIRAIHPFD AAWALPVYVA NFILMEYGTG AIFGCPAHDQ
360 370 380 390 400
RDLDFVNKYG LGNTPVVCPE GQDPASFVIT DTAYDGDGRM INSRFLDGMS
410 420 430 440 450
ADAAKEEVAK RLEGETRGNE PVAERKVNFR LRDWGISRQR YWGCPIPVIH
460 470 480 490 500
CPNCDVVPVP EKDLPVTLPE DVTFDRPGNA LDHHPTWKHV DCPKCGGKAT
510 520 530 540 550
RETDTMDTFV DSSWYFARFT DPWNDSAPTT REVADRMLPV DQYIGGVEHA
560 570 580 590 600
ILHLLYSRFF TRAMKATGHL GMDEPFKGMF TQGMVVHETY RKPDGGWASP
610 620 630 640 650
EEVRIEVDGN NRRATLITTG EPVEIGAVEK MSKSKRNTVD PDDIIGSYGA
660 670 680 690 700
DTARWFMLSD SPPDRDVIWS EEGVQGAARF MQRLWRLVNE AADAGKAAPQ
710 720 730 740 750
DRPATFGSDA LTLRKAAHGA LDRVSTGIER LHFNVCLANI REFANELADA
760 770 780 790 800
LARSRSNKSA PARDLAPDLS WSLREAAIIL VQIFSPMMPH LAEECWQRAL
810 820 830 840 850
GQTGLVSQAR WPQIEPDLLI EDSITLPVQV NGKKRGEVTV ASDAGNPEIE
860 870 880
AAVLALDAVK QALGGKQARK IIIVPRRIVS VVG
Length:883
Mass (Da):98,985
Last modified:October 11, 2005 - v1
Checksum:i61369988A74A46CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000115 Genomic DNA. Translation: ABA03359.1.
RefSeqiWP_011313428.1. NC_007406.1.
YP_316711.1. NC_007406.1.

Genome annotation databases

EnsemblBacteriaiABA03359; ABA03359; Nwi_0091.
KEGGinwi:Nwi_0091.
PATRICi22696497. VBINitWin102302_0104.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000115 Genomic DNA. Translation: ABA03359.1.
RefSeqiWP_011313428.1. NC_007406.1.
YP_316711.1. NC_007406.1.

3D structure databases

ProteinModelPortaliQ3SWI2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323098.Nwi_0091.

Proteomic databases

PRIDEiQ3SWI2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA03359; ABA03359; Nwi_0091.
KEGGinwi:Nwi_0091.
PATRICi22696497. VBINitWin102302_0104.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciNWIN323098:GJEG-92-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255."
    Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J.
    Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nb-255 / ATCC 25391.

Entry informationi

Entry nameiSYL_NITWN
AccessioniPrimary (citable) accession number: Q3SWI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 11, 2005
Last modified: April 29, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.