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Q3SWI2 (SYL_NITWN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Nwi_0091
OrganismNitrobacter winogradskyi (strain Nb-255 / ATCC 25391) [Complete proteome] [HAMAP]
Taxonomic identifier323098 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter

Protein attributes

Sequence length883 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 883883Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009383

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif630 – 6345"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6331ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3SWI2 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 61369988A74A46CD

FASTA88398,985
        10         20         30         40         50         60 
MTSERYNARD AEPRWQAEWD RQAIFATKND DPREKYYVLE MFPYPSGRIH IGHVRNYTLG 

        70         80         90        100        110        120 
DVIARYMRAR GYNVLHPMGW DAFGLPAENA AIERKIAPKA WTYDNIKAMK KQLRSIGLSL 

       130        140        150        160        170        180 
DWSREIATCD PAYYKHQQKM FLDFLRAGLA EREKRKINWD PVDMTVLANE QVIDGRGWRS 

       190        200        210        220        230        240 
GAVVEQREMN QWVFKITKFS QELLDALGTL DRWPDKVRLM QRNWIGRSEG LLIRFALDAT 

       250        260        270        280        290        300 
TTPNNESELK IFTTRPDTLF GARFMAIAPD HPLAQAAAKD NPALAGFIAE CKQRGTAQAE 

       310        320        330        340        350        360 
IDTAEKMGFD TGIRAIHPFD AAWALPVYVA NFILMEYGTG AIFGCPAHDQ RDLDFVNKYG 

       370        380        390        400        410        420 
LGNTPVVCPE GQDPASFVIT DTAYDGDGRM INSRFLDGMS ADAAKEEVAK RLEGETRGNE 

       430        440        450        460        470        480 
PVAERKVNFR LRDWGISRQR YWGCPIPVIH CPNCDVVPVP EKDLPVTLPE DVTFDRPGNA 

       490        500        510        520        530        540 
LDHHPTWKHV DCPKCGGKAT RETDTMDTFV DSSWYFARFT DPWNDSAPTT REVADRMLPV 

       550        560        570        580        590        600 
DQYIGGVEHA ILHLLYSRFF TRAMKATGHL GMDEPFKGMF TQGMVVHETY RKPDGGWASP 

       610        620        630        640        650        660 
EEVRIEVDGN NRRATLITTG EPVEIGAVEK MSKSKRNTVD PDDIIGSYGA DTARWFMLSD 

       670        680        690        700        710        720 
SPPDRDVIWS EEGVQGAARF MQRLWRLVNE AADAGKAAPQ DRPATFGSDA LTLRKAAHGA 

       730        740        750        760        770        780 
LDRVSTGIER LHFNVCLANI REFANELADA LARSRSNKSA PARDLAPDLS WSLREAAIIL 

       790        800        810        820        830        840 
VQIFSPMMPH LAEECWQRAL GQTGLVSQAR WPQIEPDLLI EDSITLPVQV NGKKRGEVTV 

       850        860        870        880 
ASDAGNPEIE AAVLALDAVK QALGGKQARK IIIVPRRIVS VVG 

« Hide

References

[1]"Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255."
Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J.
Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nb-255 / ATCC 25391.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000115 Genomic DNA. Translation: ABA03359.1.
RefSeqYP_316711.1. NC_007406.1.

3D structure databases

ProteinModelPortalQ3SWI2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323098.Nwi_0091.

Proteomic databases

PRIDEQ3SWI2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA03359; ABA03359; Nwi_0091.
GeneID3675056.
KEGGnwi:Nwi_0091.
PATRIC22696497. VBINitWin102302_0104.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycNWIN323098:GJEG-92-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_NITWN
AccessionPrimary (citable) accession number: Q3SWI2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 11, 2005
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries