Q3SW64 (AROA_NITWN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	Nwi_0209

Organism

Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) [Complete proteome] [HAMAP]

Taxonomic identifier

323098 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000058604

Sequences

	Sequence		Length	Mass (Da)
	Q3SW64 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 2596733D535D108C	FASTA	449	46,982
	10 20 30 40 50 60 MTLSGQTMPL EARKCQSLTG RVRVPGDKSI SHRALILGAL AVGETRISGL LEGEDVLNTA 70 80 90 100 110 120 KAMQALGAKV ERRTDDNGGI AWSVRGVGTG GFATPEAPLD FGNSGTGCRL VMGAVAGCPI 130 140 150 160 170 180 RAAFDGDGSL RSRPMRRILD PLELMGARVI SQSDGGRLPL TLEGARDPLP IVYRTPVASA 190 200 210 220 230 240 QIKSAVLLAG LSAPGVTTVI EREASRDHTE LMLKHFGADI VSVAEGAHGR KISLTGQPEL 250 260 270 280 290 300 HGAGVTVPAD PSSAAFPIVA ALITEGSDIV LDDVMTNPLR TGLFVTLREM GASIEESETR 310 320 330 340 350 360 LDAGEPMAQL RVKASRLRGI EVPAERAPSM IDEYLVLAVA AAFAEGTTVM RGLRELRVKE 370 380 390 400 410 420 SDRLEAAAAM LRAGGVAVEI AGDDLIVEGR GRVPGGGLVT THMDHRIAMS ALVMGCASDA 430 440 PVKVDDIAFI ATSFPDFVPM MQRLGANFA

References

[1]

"Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255." Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J. Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed: 16517654] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nb-255 / ATCC 25391.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000115 Genomic DNA. Translation: ABA03477.1.
RefSeq	YP_316829.1. NC_007406.1.
3D structure databases
HSSP	HSSP built from PDB template 2BJB based on UniProtKB P22487.
ProteinModelPortal	Q3SW64.
SMR	Q3SW64. Positions 9-448.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SW64.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3676665.
GenomeReviews	Gene locus Nwi_0209 in contig CP000115_GR.
KEGG	nwi:Nwi_0209.
NMPDR	fig|323098.3.peg.679.
PATRIC	22696759. VBINitWin102302_0233.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0128.
HOGENOM	HBG646626.
OMA	EGAHGRK.
PhylomeDB	Q3SW64.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	NWIN323098:NWI_0209-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_NITWN

Accession

Primary (citable) accession number: Q3SW64

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 11, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program