Q3SVQ1 (GLO2_NITWN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	Nwi_0373

Organism

Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) [Complete proteome] [HAMAP]

Taxonomic identifier

323098 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_0000309667
Sites
	Metal binding	56	1	Zinc 1 By similarity
	Metal binding	58	1	Zinc 1 By similarity
	Metal binding	60	1	Zinc 2 By similarity
	Metal binding	61	1	Zinc 2 By similarity
	Metal binding	114	1	Zinc 1 By similarity
	Metal binding	133	1	Zinc 1 By similarity
	Metal binding	133	1	Zinc 2 By similarity
	Metal binding	171	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SVQ1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: C50424207AEDF667	FASTA	255	28,001
	10 20 30 40 50 60 MTADIRLFTC LTDNFGVLIH DPATGATAAI DAPEAEPVIQ ALDREGWVLT DILITHHHSD 70 80 90 100 110 120 HVGGVAELKR KYACRVVAPH DRSAEIANVD LRVGQGDIIK VGTLLGRVLE TPGHTLDHVS 130 140 150 160 170 180 YVFDDEKVVF AADTLFSIGC GRVFEGTYPM MWDSLLKLRA LPDSFRLYCG HEYTDSNIKF 190 200 210 220 230 240 ALTIEPNNPV LKARAEEVTR LRAEGRPTVP TLMGEEKKAN VFLRADDPAV AAGVHMKGAS 250 GVEVFAELRE RKNKS

References

[1]

"Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255." Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J. Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed: 16517654] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nb-255 / ATCC 25391.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000115 Genomic DNA. Translation: ABA03640.1.
RefSeq	YP_316992.1. NC_007406.1.
3D structure databases
ProteinModelPortal	Q3SVQ1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SVQ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3676958.
GenomeReviews	Gene locus Nwi_0373 in contig CP000115_GR.
KEGG	nwi:Nwi_0373.
NMPDR	fig|323098.3.peg.842.
PATRIC	22697120. VBINitWin102302_0410.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	IGCGRVF.
PhylomeDB	Q3SVQ1.
ProtClustDB	CLSK818037.
Enzyme and pathway databases
BioCyc	NWIN323098:NWI_0373-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_NITWN

Accession

Primary (citable) accession number: Q3SVQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	October 11, 2005
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program