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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei398NucleophileUniRule annotation1
Active sitei451Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciNWIN323098:G1G55-1229-MONOMER
UniPathwayiUPA00164

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Nwi_1207
OrganismiNitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)
Taxonomic identifieri323098 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
Proteomesi
  • UP000002531 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002606701 – 7161,4-alpha-glucan branching enzyme GlgBAdd BLAST716

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi323098.Nwi_1207

Structurei

3D structure databases

ProteinModelPortaliQ3STC2
SMRiQ3STC2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9C Bacteria
COG0296 LUCA
HOGENOMiHOG000283037
KOiK00700
OMAiEVVHGKS
OrthoDBiPOG091H08D1

Family and domain databases

Gene3Di2.60.40.101 hit
2.60.40.11801 hit
HAMAPiMF_00685 GlgB, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR037439 Branching_enzy
IPR006407 GlgB
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PANTHERiPTHR43651 PTHR43651, 2 hits
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF02922 CBM_48, 1 hit
PIRSFiPIRSF000463 GlgB, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 2 hits
TIGRFAMsiTIGR01515 branching_enzym, 1 hit

Sequencei

Sequence statusi: Complete.

Q3STC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELSSEARA IVEGRHSDPF HYLGCHRETD RKVVRVYLPD ASEVRLIDET
60 70 80 90 100
GEEIILTRIH DAGLFLGDLP DRAERYRLRA RYGADLVELE DPYRFPPILD
110 120 130 140 150
DFDLYLLGEG THQRIYDKLG AHPMAMEGVD GAGFVVLAPN ARAVAVVGDF
160 170 180 190 200
NFWDRRRHPM RVRGNGYWEL FIPGARAGDR YKFAITAQDG SLLPLKSDPM
210 220 230 240 250
AFAAEVRPNT ASIIFDQARL PRPTPLAATI NARGVPISIY EVHLGSWRRS
260 270 280 290 300
DGNRWLSYRE LAETLPDYVR ELGFTHIELM PVNEHPFDGS WGYQPTGLYA
310 320 330 340 350
PTSRFGSPED FAHLIDACHR EGLGLLLDWV PGHFPDDPHG LGRFDGTSLY
360 370 380 390 400
EHANPLQGRH LDWDTLIYNY GRTEVVNFLV ANALFWLDRY GIDGLRVDAV
410 420 430 440 450
ASMLYLDYSR PEGGWIPNKH GGRENLEAIE FLRRFNREVF GRFPNATTVA
460 470 480 490 500
EESTAWPQVS RPIEFGGLGF GYKWNMGWMH DTLSYFEKDP IHRKHHHDQI
510 520 530 540 550
LFGLHYAFSE NFILPLSHDE VVHGKRSILG RMPGDDWQRF ANLRAYYAFM
560 570 580 590 600
FGHPGKKLMF MGAEFGQERE WNHDHALDWH LLDQARHQGV QAVVRDLNKL
610 620 630 640 650
YREVPALHEF DCDPAGFEWI VANDADHSVF AWMRKGASGR ARCLVIANLT
660 670 680 690 700
PTIHRDYRVR VPFTGRWREA LNTDSAHYGG SNVGNDGAIE TSGEIIPELV
710
LTLPPLGVIF LVPEAA
Length:716
Mass (Da):81,516
Last modified:October 11, 2005 - v1
Checksum:iA7E754D1FF133126
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000115 Genomic DNA Translation: ABA04469.1
RefSeqiWP_011314498.1, NC_007406.1

Genome annotation databases

EnsemblBacteriaiABA04469; ABA04469; Nwi_1207
KEGGinwi:Nwi_1207

Similar proteinsi

Entry informationi

Entry nameiGLGB_NITWN
AccessioniPrimary (citable) accession number: Q3STC2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 11, 2005
Last modified: February 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome