ID   Q3STC1_NITWN            Unreviewed;      1094 AA.
AC   Q3STC1;
DT   11-OCT-2005, integrated into UniProtKB/TrEMBL.
DT   11-OCT-2005, sequence version 1.
DT   27-MAR-2024, entry version 110.
DE   RecName: Full=maltose alpha-D-glucosyltransferase {ECO:0000256|ARBA:ARBA00012619};
DE            EC=5.4.99.16 {ECO:0000256|ARBA:ARBA00012619};
DE   AltName: Full=Maltose alpha-D-glucosyltransferase {ECO:0000256|ARBA:ARBA00031378};
GN   OrderedLocusNames=Nwi_1208 {ECO:0000313|EMBL:ABA04470.1};
OS   Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 /
OS   NCIMB 11846 / Nb-255).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Nitrobacteraceae; Nitrobacter.
OX   NCBI_TaxID=323098 {ECO:0000313|EMBL:ABA04470.1, ECO:0000313|Proteomes:UP000002531};
RN   [1] {ECO:0000313|EMBL:ABA04470.1, ECO:0000313|Proteomes:UP000002531}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255
RC   {ECO:0000313|Proteomes:UP000002531};
RX   PubMed=16517654; DOI=10.1128/AEM.72.3.2050-2063.2006;
RA   Starkenburg S.R., Chain P.S., Sayavedra-Soto L.A., Hauser L., Land M.L.,
RA   Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J.,
RA   Hickey W.J.;
RT   "Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium
RT   Nitrobacter winogradskyi Nb-255.";
RL   Appl. Environ. Microbiol. 72:2050-2063(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-maltose = alpha,alpha-trehalose; Xref=Rhea:RHEA:15145,
CC         ChEBI:CHEBI:16551, ChEBI:CHEBI:17306; EC=5.4.99.16;
CC         Evidence={ECO:0000256|ARBA:ARBA00001595};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. TreS
CC       subfamily. {ECO:0000256|ARBA:ARBA00005496}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000115; ABA04470.1; -; Genomic_DNA.
DR   RefSeq; WP_011314499.1; NC_007406.1.
DR   AlphaFoldDB; Q3STC1; -.
DR   STRING; 323098.Nwi_1208; -.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   KEGG; nwi:Nwi_1208; -.
DR   eggNOG; COG0366; Bacteria.
DR   eggNOG; COG3281; Bacteria.
DR   HOGENOM; CLU_007635_1_2_5; -.
DR   OrthoDB; 9805159at2; -.
DR   Proteomes; UP000002531; Chromosome.
DR   GO; GO:0047471; F:maltose alpha-D-glucosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11334; AmyAc_TreS; 1.
DR   Gene3D; 3.90.1200.10; -; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR032091; Malt_amylase_C.
DR   InterPro; IPR045857; O16G_dom_2.
DR   InterPro; IPR012810; TreS/a-amylase_N.
DR   InterPro; IPR012811; TreS_maltokin_C_dom.
DR   NCBIfam; TIGR02457; TreS_Cterm; 1.
DR   NCBIfam; TIGR02456; treS_nterm; 1.
DR   PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1.
DR   PANTHER; PTHR10357:SF219; MALTOSE ALPHA-D-GLUCOSYLTRANSFERASE; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF16657; Malt_amylase_C; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
DR   SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
PE   3: Inferred from homology;
KW   Calcium {ECO:0000256|ARBA:ARBA00022837};
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:ABA04470.1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002531}.
FT   DOMAIN          27..426
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   1094 AA;  124586 MW;  8EA29E353D0B3A97 CRC64;
     MNEMSHIDAD RYVQTDDLWY KDAIIYQLHV KAFADSNNDG IGDFVGLTNK LDYLQELGVT
     ALWLLPFYPS PGRDDGYDIA DYGSVNPDFG TMKDFRRFIS EAKRRGLRVI TELVINHTSD
     QHRWFKRAQR SPAGSSARNW YVWSNTDKKY QGTRIIFTDT EKSNWAWDSE ANAYYWHRFF
     SHQPDLNFDN PRVVRAVIQV MKRWLDTGVD GFRLDAIPYL CEREGTNNEN LPETHAVIKH
     IRAALDSYAK GKVLLAEANQ WPEDVQFYFG DGDECHMAYH FPLMPRIYMA IAQEDRFPIT
     DILRQTPDIP DNCQWAMFLR NHDELTLEMV TDIERDYLWS TYAADPRARI NLGIRRRLAP
     LMDNDRRKIE LMNSLLMSLP GTPIIYYGDE IGMGDNIYLG DRNGVRTPMQ WTSDRNGGFS
     RCDPARLYAP PVMDAVYGYE SVNVESQSRS LSSLLSWMKK LISVRKSSHV FGRGTMAFVR
     SSNRSVLAYL RQHGDEAVLC VANLSRSAQA SELDLSHWKG RSPLEMLGRT TFPDIGELPY
     LITLAPYGFY WFRLNEKPPS PNVAAVIVPD YETLVVPQGA TWMSLARTRS IFERDVLPSH
     LARSRWFPER SAGAITARVP AAIPLTKDQV SRPWLSIFDV THRGQTGRYA MPIQIRWDRL
     DRKNYDARNV AAVRQGSREG TLIDAAYESG LITGIIEGLR QRATFEGGNL QISYIPTERL
     ASITEEPIEN VRPVQTEQSN STALVDQKFV VKLYRKLENG INPEVEVGRF LTDVAGYANT
     PALLGSVELT GPDVHAAVAI VHAFVGNQGD AWTISSAALD RFIDEQRLVG AIDTAPALEE
     KAAYQRYMMQ LGKRVAEMHV ALASRGDIED FRPEPVNASD VAHFIDRLTA RAEKTFDRLA
     QARLSESDRI LVEEIQSLRP ALHGLLAGLL PSSIQMYSIR HHGDFHLGQV LVVRDDVFII
     DFEGEPRRSL SERRMKAPAA RDVAGLIRSI DYSVTSAQQR VLKTEVDDDG RLAEALSAWR
     DEATQTFLIA YREAMSDSRL WPQDNDESEG MLRFFLLEKA LYEIDYELAH RPDWLRVALD
     GALRVLRNDK RDRA
//