Q3SSY0 (RPOB_NITWN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	Nwi_1350

Organism

Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) [Complete proteome] [HAMAP]

Taxonomic identifier

323098 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter

Protein attributes

Sequence length	1380 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1380	1380	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000224082

Sequences

	Sequence		Length	Mass (Da)
	Q3SSY0 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: E9002B52DB7B5139	FASTA	1,380	154,232
	10 20 30 40 50 60 MVQQTFTGRK RVRKFFGHIR EVAEMPNLIE VQKASYDQFL MVDEPEGGRL DEGLQAVFKS 70 80 90 100 110 120 VFPINDFSGA SQLEFVRYEF EAPKYDVDEC RQRGMTYAAP LKVTLRLIVF DIDEETGAKS 130 140 150 160 170 180 VKDIKEQDVY MGDIPLMTMN GTFVVNGTER VIVSQMHRSP GVFFDHDKGK THSSGKLLFA 190 200 210 220 230 240 ARVIPYRGSW LDIEFDAKDI VFARIDRRRK IPVTSLMFAL GLDGEEILST FYKKIIYKRA 250 260 270 280 290 300 KSGGGSDGWR VPYDPVRFRG YSTLNDLIDA DTGKVVLEAG KKLTVRAARQ LQEKGLKALR 310 320 330 340 350 360 MSDEELVGMY LAEDLVNPKT GEIYAEAGEE ITEKSLKALN EEGYKELPLL DIDHVNVGPY 370 380 390 400 410 420 IRNTLAADKN MTREDALFDI YRVMRPGEPP TLESAQNMFQ SLFFDAERYD LSAVGRVKMN 430 440 450 460 470 480 MRLDLDAPDT YRTLRKEDIL AVIKTLVDLR DGKGEIDDID HLGNRRVRSV GELMENQYRV 490 500 510 520 530 540 GLLRMERAIK ERMSSVDIDT VMPQDLINAK PAAAAVREFF GSSQLSQFMD QTNPLSEITH 550 560 570 580 590 600 KRRLSALGPG GLTRERAGFE VRDVHPTHYG RICPIETPEG PNIGLINSLA TFARVNKYGF 610 620 630 640 650 660 VETPYRKVKD GRVTDEVVYL SAMEEGRYHV AQANLPLDAR GRFTEDLVVC RHAGEVLPVT 670 680 690 700 710 720 PDKVDFMDVS PKQLVSVAAA LIPFLENDDA NRALMGSNMQ RQAVPLVRAE APFVGTGMEG 730 740 750 760 770 780 VVARDSGAAI AARRSGVIDQ IDATRVVIRA TEDLDPTKSG VDIYRLMKYQ RSNQSTCINQ 790 800 810 820 830 840 RPLVKVGDIV RKGDIIADGP STDLGELALG RNVLVAFMPW NGYNFEDSIL LSERIVKEDV 850 860 870 880 890 900 FTSIHIEEFE VMARDTKLGP EEITRDIPNV SEEALKSLDE AGIVYIGAEV RAGDILVGKI 910 920 930 940 950 960 TPKGESPMTP EEKLLRAIFG EKASDVRDTS LRVPPGVQGT IVEVRVFNRH GVDKDERALA 970 980 990 1000 1010 1020 IEREEIERLA KDRDDEQAIL DRNVYGRLAD LLENRQGIAG PKGFKKDTKI TRAVLDEYPK 1030 1040 1050 1060 1070 1080 SQWWLFASPN DKLMAEIEAM RKQYDESKKG LEQRFLDKVE KLQRGDELPP GVMKMVKVFV 1090 1100 1110 1120 1130 1140 AVKRKIQPGD KMAGRHGNKG VVSKIVPIED MPFLEDGTHA DIVLNPLGVP SRMNVGQILE 1150 1160 1170 1180 1190 1200 THLGWACAGL GRRIGQAVDA YLASAKQETK PLKETLKKVY GDNETIKSLE DHELVELGRN 1210 1220 1230 1240 1250 1260 LRRGVPIATP VFDGAKEADI EQMLELAGMD KSGQSTVYDG RTGDPFDRKV TVGYIYMLKL 1270 1280 1290 1300 1310 1320 HHLVDDKIHA RSIGPYSLVT QQPLGGKAQF GGQRFGEMEV WALEAYGAAY TLQEMLTVKS 1330 1340 1350 1360 1370 1380 DDVAGRTKVY EAIVRGDDTF EAGIPESFNV LVKEMRSLGL NVDLHNSKIG DMMPTSEAAE

References

[1]

"Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255." Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J. Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed: 16517654] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nb-255 / ATCC 25391.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000115 Genomic DNA. Translation: ABA04611.1.
RefSeq	YP_317963.1. NC_007406.1.
3D structure databases
ProteinModelPortal	Q3SSY0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SSY0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3675767.
GenomeReviews	Gene locus Nwi_1350 in contig CP000115_GR.
KEGG	nwi:Nwi_1350.
NMPDR	fig|323098.3.peg.2218.
PATRIC	22699364. VBINitWin102302_1514.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0085.
HOGENOM	HBG285547.
OMA	QRDLTYA.
PhylomeDB	Q3SSY0.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	NWIN323098:NWI_1350-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_NITWN

Accession

Primary (citable) accession number: Q3SSY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	October 11, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program