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Protein

Protein-L-isoaspartate O-methyltransferase

Gene

pcm

Organism
Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei64UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-L-isoaspartate O-methyltransferaseUniRule annotation (EC:2.1.1.77UniRule annotation)
Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferaseUniRule annotation
Protein L-isoaspartyl methyltransferaseUniRule annotation
Protein-beta-aspartate methyltransferaseUniRule annotation
Short name:
PIMTUniRule annotation
Gene namesi
Name:pcmUniRule annotation
Ordered Locus Names:Nwi_1773
OrganismiNitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)
Taxonomic identifieri323098 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
Proteomesi
  • UP000002531 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003518841 – 217Protein-L-isoaspartate O-methyltransferaseAdd BLAST217

Interactioni

Protein-protein interaction databases

STRINGi323098.Nwi_1773.

Structurei

3D structure databases

ProteinModelPortaliQ3SRQ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E26. Bacteria.
COG2518. LUCA.
HOGENOMiHOG000257189.
KOiK00573.
OMAiRDQFVDP.
OrthoDBiPOG091H06AY.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00090. PIMT. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3SRQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPADHPPPE KMMFQLNLRR RGISDQRVLR AMDNVPRDAF VEQGDREDAW
60 70 80 90 100
CDTALGIACG QTISQPFVVA YMTERLELGD DQRVLEIGTG SGYQTAILSR
110 120 130 140 150
LCREVVTVER YRVLADRARA RLKHLGYDNV EVLLGDGFDI PGEAGRFDRI
160 170 180 190 200
MVTAAMEQIP EALTARLEPD GLLIAPVGPQ SGRQTLILLR RAPAGMIRKE
210
LIDVRFVPAL PGIAREL
Length:217
Mass (Da):24,159
Last modified:October 11, 2005 - v1
Checksum:iDB7076350B94A3FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000115 Genomic DNA. Translation: ABA05034.1.
RefSeqiWP_011315030.1. NC_007406.1.

Genome annotation databases

EnsemblBacteriaiABA05034; ABA05034; Nwi_1773.
KEGGinwi:Nwi_1773.
PATRICi22700322. VBINitWin102302_1984.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000115 Genomic DNA. Translation: ABA05034.1.
RefSeqiWP_011315030.1. NC_007406.1.

3D structure databases

ProteinModelPortaliQ3SRQ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323098.Nwi_1773.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA05034; ABA05034; Nwi_1773.
KEGGinwi:Nwi_1773.
PATRICi22700322. VBINitWin102302_1984.

Phylogenomic databases

eggNOGiENOG4105E26. Bacteria.
COG2518. LUCA.
HOGENOMiHOG000257189.
KOiK00573.
OMAiRDQFVDP.
OrthoDBiPOG091H06AY.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00090. PIMT. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPIMT_NITWN
AccessioniPrimary (citable) accession number: Q3SRQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.