Q3SRK0 (KDSA_NITWN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase
EC=2.5.1.55

Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
KDO-8-phosphate synthase
Short name=KDO 8-P synthase
Short name=KDOPS
Phospho-2-dehydro-3-deoxyoctonate aldolase

Gene names

Name:	kdsA
Ordered Locus Names:	Nwi_1831

Organism

Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) [Complete proteome] [HAMAP]

Taxonomic identifier

323098 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the KdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_0000304463

Sequences

	Sequence		Length	Mass (Da)
	Q3SRK0 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 606089DCE904CDBC	FASTA	287	29,822
	10 20 30 40 50 60 MNQPVSASPV VSVGSVTFGQ DRPLSIIAGP CQMESRAHAL EVAGALKDIA ARLNVGLVFK 70 80 90 100 110 120 TSFDKANRTS ASGARGIGLK QALPVFADIR SSLGLPVLTD VHEAAQCAEV AQVVDVLQIP 130 140 150 160 170 180 AFLCRQTDLL LAAAATGKVV NVKKGQFLAP WDMGNVVAKI TGGGNRNILV TERGASFGYN 190 200 210 220 230 240 TLVSDMRALP ILARTTGAPV IFDATHSVQQ PGGKGASTGG EREFVPVLAR AAVAVGVAGV 250 260 270 280 FIETHPDPDH APSDGPNMVP LREFEALVRR LMAFDALAKA ADPACPE

References

[1]

"Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255." Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J. Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed: 16517654] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nb-255 / ATCC 25391.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000115 Genomic DNA. Translation: ABA05091.1.
RefSeq	YP_318443.1. NC_007406.1.
3D structure databases
HSSP	HSSP built from PDB template 2A21 based on UniProtKB O66496.
ProteinModelPortal	Q3SRK0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SRK0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3674756.
GenomeReviews	Gene locus Nwi_1831 in contig CP000115_GR.
KEGG	nwi:Nwi_1831.
NMPDR	fig|323098.3.peg.1885.
PATRIC	22700468. VBINitWin102302_2055.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2877.
HOGENOM	HBG575990.
OMA	DVHEAAQ.
PhylomeDB	Q3SRK0.
ProtClustDB	PRK05198.
Enzyme and pathway databases
BioCyc	NWIN323098:NWI_1831-MONOMER.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01627.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_NITWN

Accession

Primary (citable) accession number: Q3SRK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 11, 2005
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program