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Q3SRI8 (SYQ_NITWN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:Nwi_1843
OrganismNitrobacter winogradskyi (strain Nb-255 / ATCC 25391) [Complete proteome] [HAMAP]
Taxonomic identifier323098 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter

Protein attributes

Sequence length559 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 559559Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_1000095495

Regions

Motif36 – 4611"HIGH" region HAMAP-Rule MF_00126
Motif270 – 2745"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site2731ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3SRI8 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: DF09B74C14F9C3DA

FASTA55963,415
        10         20         30         40         50         60 
MTDESTAEAG RDFIRDIVAA DLASGRHKSI VTRFPPEPNG YLHLGHAKSI CLNFGIAEDF 

        70         80         90        100        110        120 
GGRCHLRFDD TNPAKEEQEF IDAIQRDVRW LGFDWGEHLY YASDYFEQLY AWAQHLIREG 

       130        140        150        160        170        180 
KAYVDDQTQE EIRLARGTLT EPGRNSPFRD RPADENLDLF ARMRAGEFPN GARVLRAKID 

       190        200        210        220        230        240 
MAAGNINLRD PVLYRILHAT HPRTGNVWSI YPSYDFAHGQ SDSIEHITHS VCTLEFEDHR 

       250        260        270        280        290        300 
PLYDWFLDNL PVPSRPRQYE FARLNVTHTL LSKRVLTELV RGGHVAGWDD PRMPTLAGLQ 

       310        320        330        340        350        360 
RRGVPAEALR EFVKRIGVAK ANSTVDVGML DFAIREALNR SASRRMAVLR PLKVVIENYP 

       370        380        390        400        410        420 
EGQSEELEAV NHPDDPSQGT RRIRFGREIY IEQEDFMENP PKKFFRLSPG REVRLRYAYF 

       430        440        450        460        470        480 
ITCREVVKNP AGDVLELRCT YDPATRGGNA PDGRKVKATM HWVNTADAVP AEVRLYGHLF 

       490        500        510        520        530        540 
ETEQPDAANF ASELNPQSVQ ILSGCMVEPA LARDDAGAAV QFERQGYFYR DRDSAPGRLV 

       550 
FNRTVGLRDT WAKVAAAGS 

« Hide

References

[1]"Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255."
Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J.
Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nb-255 / ATCC 25391.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000115 Genomic DNA. Translation: ABA05103.1.
RefSeqYP_318455.1. NC_007406.1.

3D structure databases

ProteinModelPortalQ3SRI8.
SMRQ3SRI8. Positions 12-550.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323098.Nwi_1843.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA05103; ABA05103; Nwi_1843.
GeneID3674814.
KEGGnwi:Nwi_1843.
PATRIC22700494. VBINitWin102302_2068.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000259232.
KOK01886.
OMAVTHSICT.
OrthoDBEOG6DRPF7.
ProtClustDBPRK05347.

Enzyme and pathway databases

BioCycNWIN323098:GJEG-1878-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_NITWN
AccessionPrimary (citable) accession number: Q3SRI8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 11, 2005
Last modified: February 19, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries