Q3SPW7 (BIOD_NITWN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	Nwi_2420

Organism

Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) [Complete proteome] [HAMAP]

Taxonomic identifier

323098 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000302536
Regions
	Nucleotide binding	100 – 103	4	ATP By similarity
Sites
	Metal binding	17	1	Magnesium 2 By similarity
	Metal binding	100	1	Magnesium 2 By similarity
	Binding site	37	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SPW7 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 759B2A14F34EA687	FASTA	213	23,168
	10 20 30 40 50 60 MSARIVVSGT DTGVGKTVFS AALAGALDGY YWKPVQSGLD EETDSQTVMR LSGLGAERVW 70 80 90 100 110 120 PEAYRLRTPV SPHLAAELDG VTIDHRALEA PHTDRPLVIE GAGGLLVPLT RDVTYLDLMA 130 140 150 160 170 180 RWRLPVVLCA RTALGTINHS LLSIEALRAR DVAILGVSFI GEENVETEGI IIDMGRVKRL 190 200 210 GRLSYVTPLT RATLRQAFAD GFDIGDFVKE RAR

References

[1]

"Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255." Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J. Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed: 16517654] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nb-255 / ATCC 25391.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000115 Genomic DNA. Translation: ABA05674.1.
RefSeq	YP_319026.1. NC_007406.1.
3D structure databases
ProteinModelPortal	Q3SPW7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SPW7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3676533.
GenomeReviews	Gene locus Nwi_2420 in contig CP000115_GR.
KEGG	nwi:Nwi_2420.
NMPDR	fig|323098.3.peg.2320.
PATRIC	22701816. VBINitWin102302_2719.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0132.
HOGENOM	HBG650065.
OMA	WKPIQSG.
PhylomeDB	Q3SPW7.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	NWIN323098:NWI_2420-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_NITWN

Accession

Primary (citable) accession number: Q3SPW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 11, 2005
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program