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Q3SPJ6 (SYI_NITWN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:Nwi_2542
OrganismNitrobacter winogradskyi (strain Nb-255 / ATCC 25391) [Complete proteome] [HAMAP]
Taxonomic identifier323098 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter

Protein attributes

Sequence length999 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 999999Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_0000098430

Regions

Motif67 – 7711"HIGH" region HAMAP-Rule MF_02002
Motif668 – 6725"KMSKS" region HAMAP-Rule MF_02002

Sites

Binding site6271Aminoacyl-adenylate By similarity
Binding site6711ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3SPJ6 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 689891A0DB8D55C8

FASTA999111,640
        10         20         30         40         50         60 
MTEKKKNSDA PDYAKTLFLP QTEFPMRAGL PQREPDILKR WTDIDLYGQL RACAKGRPKF 

        70         80         90        100        110        120 
VLHDGPPYAN GSIHIGHALN KILKDVVTKS QQMLGHDSNY VPGWDCHGLP IEWKIEEENY 

       130        140        150        160        170        180 
RSKGKTKPNF KDPAAMVAFR KECRAYAEHW LNVQREQFKR LGIIGDWDHP YATMTYPAEA 

       190        200        210        220        230        240 
QIARELMKFA ANGTLYRGSK PVMWSVVEKT ALAEAEVEYE DHTSDTVWVK FPVTSPAHGA 

       250        260        270        280        290        300 
LSQASVVIWT TTPWTLPGNR AISFSNKIAY GLYKITDAPA DNWAKTGDVL ILANKLAGEV 

       310        320        330        340        350        360 
FKQARVTAYE KVRDIPADTL DAVECAHPLK GLSGGYEFTV PLLDGDHVTD DTGTGFVHTA 

       370        380        390        400        410        420 
PGHGREDFDI WTANARELEV RGISSTIPYT VDENGALTAQ APGFEGKRVI DDKGNKGDAN 

       430        440        450        460        470        480 
EAVIKALVER NRLLARGRLK HQYPHSWRSK KPVIFRNTPQ WFIAMDRDIA DDGKANHGDT 

       490        500        510        520        530        540 
LRARALQAIS VTRWVPPAGQ NRITGMIANR PDWVISRQRA WGVPIAVFVR EKGDGSAEVL 

       550        560        570        580        590        600 
IDDAVNRRIS DAFEKEGADA WYAEGARERF LGDRAGEDWS KVDDILDVWF DSGSTHAFVL 

       610        620        630        640        650        660 
EDPAHFPSLR DIKRKADGGR DTVMYLEGSD QHRGWFHSSL LESCGTRGRA PYDIVLTHGF 

       670        680        690        700        710        720 
TLDENGRKMS KSLGNTVDPQ KVIKDSGADI LRLWVCATDY ADDQRIGPEI LKNTIETYRK 

       730        740        750        760        770        780 
LRNTIRWMLG TLHHFDRGEA VAFTEMPELE RLMLHRLAEQ SALVRSAYAE FDYKTVVASL 

       790        800        810        820        830        840 
AAFMNTELSA FYFDIRKDTL YCDPPSSPAR KAALTVIDKL CDAILKWLAP VLSFTTEEAW 

       850        860        870        880        890        900 
RLYRPDAEPS VHLTLFPESF DNFRDDSLAA KWETVRAVRS VVTGALELER AAKRIGSSLE 

       910        920        930        940        950        960 
ASPIVHVADR AMLGTLFDID LAEVCITSNF EVREGDAPDG AFRLPNVQGV AVVVEKAAGT 

       970        980        990 
KCARSWKILP TVGSDSEYPD VSPRDAQALR EWKALGVTV 

« Hide

References

[1]"Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255."
Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J.
Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nb-255 / ATCC 25391.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000115 Genomic DNA. Translation: ABA05795.1.
RefSeqYP_319147.1. NC_007406.1.

3D structure databases

ProteinModelPortalQ3SPJ6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323098.Nwi_2542.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA05795; ABA05795; Nwi_2542.
GeneID3675257.
KEGGnwi:Nwi_2542.
PATRIC22702106. VBINitWin102302_2861.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAYETLYEC.
OrthoDBEOG644ZM1.

Enzyme and pathway databases

BioCycNWIN323098:GJEG-2591-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_NITWN
AccessionPrimary (citable) accession number: Q3SPJ6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 11, 2005
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries