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Q3SPJ6

- SYI_NITWN

UniProt

Q3SPJ6 - SYI_NITWN

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Nwi_2542
Organism
Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei627 – 6271Aminoacyl-adenylate By similarity
Binding sitei671 – 6711ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNWIN323098:GJEG-2591-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Nwi_2542
OrganismiNitrobacter winogradskyi (strain Nb-255 / ATCC 25391)
Taxonomic identifieri323098 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
ProteomesiUP000002531: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 999999Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098430Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi323098.Nwi_2542.

Structurei

3D structure databases

ProteinModelPortaliQ3SPJ6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi67 – 7711"HIGH" regionUniRule annotation
Add
BLAST
Motifi668 – 6725"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiYETLYEC.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3SPJ6-1 [UniParc]FASTAAdd to Basket

« Hide

MTEKKKNSDA PDYAKTLFLP QTEFPMRAGL PQREPDILKR WTDIDLYGQL    50
RACAKGRPKF VLHDGPPYAN GSIHIGHALN KILKDVVTKS QQMLGHDSNY 100
VPGWDCHGLP IEWKIEEENY RSKGKTKPNF KDPAAMVAFR KECRAYAEHW 150
LNVQREQFKR LGIIGDWDHP YATMTYPAEA QIARELMKFA ANGTLYRGSK 200
PVMWSVVEKT ALAEAEVEYE DHTSDTVWVK FPVTSPAHGA LSQASVVIWT 250
TTPWTLPGNR AISFSNKIAY GLYKITDAPA DNWAKTGDVL ILANKLAGEV 300
FKQARVTAYE KVRDIPADTL DAVECAHPLK GLSGGYEFTV PLLDGDHVTD 350
DTGTGFVHTA PGHGREDFDI WTANARELEV RGISSTIPYT VDENGALTAQ 400
APGFEGKRVI DDKGNKGDAN EAVIKALVER NRLLARGRLK HQYPHSWRSK 450
KPVIFRNTPQ WFIAMDRDIA DDGKANHGDT LRARALQAIS VTRWVPPAGQ 500
NRITGMIANR PDWVISRQRA WGVPIAVFVR EKGDGSAEVL IDDAVNRRIS 550
DAFEKEGADA WYAEGARERF LGDRAGEDWS KVDDILDVWF DSGSTHAFVL 600
EDPAHFPSLR DIKRKADGGR DTVMYLEGSD QHRGWFHSSL LESCGTRGRA 650
PYDIVLTHGF TLDENGRKMS KSLGNTVDPQ KVIKDSGADI LRLWVCATDY 700
ADDQRIGPEI LKNTIETYRK LRNTIRWMLG TLHHFDRGEA VAFTEMPELE 750
RLMLHRLAEQ SALVRSAYAE FDYKTVVASL AAFMNTELSA FYFDIRKDTL 800
YCDPPSSPAR KAALTVIDKL CDAILKWLAP VLSFTTEEAW RLYRPDAEPS 850
VHLTLFPESF DNFRDDSLAA KWETVRAVRS VVTGALELER AAKRIGSSLE 900
ASPIVHVADR AMLGTLFDID LAEVCITSNF EVREGDAPDG AFRLPNVQGV 950
AVVVEKAAGT KCARSWKILP TVGSDSEYPD VSPRDAQALR EWKALGVTV 999
Length:999
Mass (Da):111,640
Last modified:October 11, 2005 - v1
Checksum:i689891A0DB8D55C8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000115 Genomic DNA. Translation: ABA05795.1.
RefSeqiWP_011315746.1. NC_007406.1.
YP_319147.1. NC_007406.1.

Genome annotation databases

EnsemblBacteriaiABA05795; ABA05795; Nwi_2542.
GeneIDi3675257.
KEGGinwi:Nwi_2542.
PATRICi22702106. VBINitWin102302_2861.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000115 Genomic DNA. Translation: ABA05795.1 .
RefSeqi WP_011315746.1. NC_007406.1.
YP_319147.1. NC_007406.1.

3D structure databases

ProteinModelPortali Q3SPJ6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 323098.Nwi_2542.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABA05795 ; ABA05795 ; Nwi_2542 .
GeneIDi 3675257.
KEGGi nwi:Nwi_2542.
PATRICi 22702106. VBINitWin102302_2861.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi YETLYEC.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci NWIN323098:GJEG-2591-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255."
    Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J.
    Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nb-255 / ATCC 25391.

Entry informationi

Entry nameiSYI_NITWN
AccessioniPrimary (citable) accession number: Q3SPJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 11, 2005
Last modified: September 3, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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