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Q3SPJ6

- SYI_NITWN

UniProt

Q3SPJ6 - SYI_NITWN

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (11 Oct 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei627 – 6271Aminoacyl-adenylateUniRule annotation
    Binding sitei671 – 6711ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciNWIN323098:GJEG-2591-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Nwi_2542
    OrganismiNitrobacter winogradskyi (strain Nb-255 / ATCC 25391)
    Taxonomic identifieri323098 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
    ProteomesiUP000002531: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 999999Isoleucine--tRNA ligasePRO_0000098430Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi323098.Nwi_2542.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3SPJ6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi67 – 7711"HIGH" regionAdd
    BLAST
    Motifi668 – 6725"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiYETLYEC.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3SPJ6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEKKKNSDA PDYAKTLFLP QTEFPMRAGL PQREPDILKR WTDIDLYGQL    50
    RACAKGRPKF VLHDGPPYAN GSIHIGHALN KILKDVVTKS QQMLGHDSNY 100
    VPGWDCHGLP IEWKIEEENY RSKGKTKPNF KDPAAMVAFR KECRAYAEHW 150
    LNVQREQFKR LGIIGDWDHP YATMTYPAEA QIARELMKFA ANGTLYRGSK 200
    PVMWSVVEKT ALAEAEVEYE DHTSDTVWVK FPVTSPAHGA LSQASVVIWT 250
    TTPWTLPGNR AISFSNKIAY GLYKITDAPA DNWAKTGDVL ILANKLAGEV 300
    FKQARVTAYE KVRDIPADTL DAVECAHPLK GLSGGYEFTV PLLDGDHVTD 350
    DTGTGFVHTA PGHGREDFDI WTANARELEV RGISSTIPYT VDENGALTAQ 400
    APGFEGKRVI DDKGNKGDAN EAVIKALVER NRLLARGRLK HQYPHSWRSK 450
    KPVIFRNTPQ WFIAMDRDIA DDGKANHGDT LRARALQAIS VTRWVPPAGQ 500
    NRITGMIANR PDWVISRQRA WGVPIAVFVR EKGDGSAEVL IDDAVNRRIS 550
    DAFEKEGADA WYAEGARERF LGDRAGEDWS KVDDILDVWF DSGSTHAFVL 600
    EDPAHFPSLR DIKRKADGGR DTVMYLEGSD QHRGWFHSSL LESCGTRGRA 650
    PYDIVLTHGF TLDENGRKMS KSLGNTVDPQ KVIKDSGADI LRLWVCATDY 700
    ADDQRIGPEI LKNTIETYRK LRNTIRWMLG TLHHFDRGEA VAFTEMPELE 750
    RLMLHRLAEQ SALVRSAYAE FDYKTVVASL AAFMNTELSA FYFDIRKDTL 800
    YCDPPSSPAR KAALTVIDKL CDAILKWLAP VLSFTTEEAW RLYRPDAEPS 850
    VHLTLFPESF DNFRDDSLAA KWETVRAVRS VVTGALELER AAKRIGSSLE 900
    ASPIVHVADR AMLGTLFDID LAEVCITSNF EVREGDAPDG AFRLPNVQGV 950
    AVVVEKAAGT KCARSWKILP TVGSDSEYPD VSPRDAQALR EWKALGVTV 999
    Length:999
    Mass (Da):111,640
    Last modified:October 11, 2005 - v1
    Checksum:i689891A0DB8D55C8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000115 Genomic DNA. Translation: ABA05795.1.
    RefSeqiWP_011315746.1. NC_007406.1.
    YP_319147.1. NC_007406.1.

    Genome annotation databases

    EnsemblBacteriaiABA05795; ABA05795; Nwi_2542.
    GeneIDi3675257.
    KEGGinwi:Nwi_2542.
    PATRICi22702106. VBINitWin102302_2861.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000115 Genomic DNA. Translation: ABA05795.1 .
    RefSeqi WP_011315746.1. NC_007406.1.
    YP_319147.1. NC_007406.1.

    3D structure databases

    ProteinModelPortali Q3SPJ6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 323098.Nwi_2542.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABA05795 ; ABA05795 ; Nwi_2542 .
    GeneIDi 3675257.
    KEGGi nwi:Nwi_2542.
    PATRICi 22702106. VBINitWin102302_2861.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi YETLYEC.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci NWIN323098:GJEG-2591-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255."
      Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J.
      Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Nb-255 / ATCC 25391.

    Entry informationi

    Entry nameiSYI_NITWN
    AccessioniPrimary (citable) accession number: Q3SPJ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3