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Q3SPJ6

- SYI_NITWN

UniProt

Q3SPJ6 - SYI_NITWN

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei627 – 6271Aminoacyl-adenylateUniRule annotation
Binding sitei671 – 6711ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNWIN323098:GJEG-2591-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Nwi_2542
OrganismiNitrobacter winogradskyi (strain Nb-255 / ATCC 25391)
Taxonomic identifieri323098 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
ProteomesiUP000002531: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 999999Isoleucine--tRNA ligasePRO_0000098430Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi323098.Nwi_2542.

Structurei

3D structure databases

ProteinModelPortaliQ3SPJ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi67 – 7711"HIGH" regionAdd
BLAST
Motifi668 – 6725"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiYETLYEC.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3SPJ6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEKKKNSDA PDYAKTLFLP QTEFPMRAGL PQREPDILKR WTDIDLYGQL
60 70 80 90 100
RACAKGRPKF VLHDGPPYAN GSIHIGHALN KILKDVVTKS QQMLGHDSNY
110 120 130 140 150
VPGWDCHGLP IEWKIEEENY RSKGKTKPNF KDPAAMVAFR KECRAYAEHW
160 170 180 190 200
LNVQREQFKR LGIIGDWDHP YATMTYPAEA QIARELMKFA ANGTLYRGSK
210 220 230 240 250
PVMWSVVEKT ALAEAEVEYE DHTSDTVWVK FPVTSPAHGA LSQASVVIWT
260 270 280 290 300
TTPWTLPGNR AISFSNKIAY GLYKITDAPA DNWAKTGDVL ILANKLAGEV
310 320 330 340 350
FKQARVTAYE KVRDIPADTL DAVECAHPLK GLSGGYEFTV PLLDGDHVTD
360 370 380 390 400
DTGTGFVHTA PGHGREDFDI WTANARELEV RGISSTIPYT VDENGALTAQ
410 420 430 440 450
APGFEGKRVI DDKGNKGDAN EAVIKALVER NRLLARGRLK HQYPHSWRSK
460 470 480 490 500
KPVIFRNTPQ WFIAMDRDIA DDGKANHGDT LRARALQAIS VTRWVPPAGQ
510 520 530 540 550
NRITGMIANR PDWVISRQRA WGVPIAVFVR EKGDGSAEVL IDDAVNRRIS
560 570 580 590 600
DAFEKEGADA WYAEGARERF LGDRAGEDWS KVDDILDVWF DSGSTHAFVL
610 620 630 640 650
EDPAHFPSLR DIKRKADGGR DTVMYLEGSD QHRGWFHSSL LESCGTRGRA
660 670 680 690 700
PYDIVLTHGF TLDENGRKMS KSLGNTVDPQ KVIKDSGADI LRLWVCATDY
710 720 730 740 750
ADDQRIGPEI LKNTIETYRK LRNTIRWMLG TLHHFDRGEA VAFTEMPELE
760 770 780 790 800
RLMLHRLAEQ SALVRSAYAE FDYKTVVASL AAFMNTELSA FYFDIRKDTL
810 820 830 840 850
YCDPPSSPAR KAALTVIDKL CDAILKWLAP VLSFTTEEAW RLYRPDAEPS
860 870 880 890 900
VHLTLFPESF DNFRDDSLAA KWETVRAVRS VVTGALELER AAKRIGSSLE
910 920 930 940 950
ASPIVHVADR AMLGTLFDID LAEVCITSNF EVREGDAPDG AFRLPNVQGV
960 970 980 990
AVVVEKAAGT KCARSWKILP TVGSDSEYPD VSPRDAQALR EWKALGVTV
Length:999
Mass (Da):111,640
Last modified:October 11, 2005 - v1
Checksum:i689891A0DB8D55C8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000115 Genomic DNA. Translation: ABA05795.1.
RefSeqiWP_011315746.1. NC_007406.1.
YP_319147.1. NC_007406.1.

Genome annotation databases

EnsemblBacteriaiABA05795; ABA05795; Nwi_2542.
GeneIDi3675257.
KEGGinwi:Nwi_2542.
PATRICi22702106. VBINitWin102302_2861.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000115 Genomic DNA. Translation: ABA05795.1 .
RefSeqi WP_011315746.1. NC_007406.1.
YP_319147.1. NC_007406.1.

3D structure databases

ProteinModelPortali Q3SPJ6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 323098.Nwi_2542.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABA05795 ; ABA05795 ; Nwi_2542 .
GeneIDi 3675257.
KEGGi nwi:Nwi_2542.
PATRICi 22702106. VBINitWin102302_2861.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi YETLYEC.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci NWIN323098:GJEG-2591-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255."
    Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J.
    Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nb-255 / ATCC 25391.

Entry informationi

Entry nameiSYI_NITWN
AccessioniPrimary (citable) accession number: Q3SPJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 11, 2005
Last modified: October 29, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3