Q3SPE5 (ISPE_NITWN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Nwi_2593

Organism

Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) [Complete proteome] [HAMAP]

Taxonomic identifier

323098 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235106
Regions
	Nucleotide binding	108 – 118	11	ATP Potential
Sites
	Active site	23	1	By similarity
	Active site	150	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SPE5 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 0B9E1D5C51DB7EB2	FASTA	308	31,932
	10 20 30 40 50 60 MIVTGATDLE TAAPALSETA RAKVNLTLRV VGRRADGFHD LESVVAFADC VDRLTLTQGA 70 80 90 100 110 120 ELSLVAAGPR AQECGQTADN LVLTAARLLG ERIPDLKAGA FALDKYLPVA AGIGGGSADA 130 140 150 160 170 180 AAALRLLARA NGLSIDDPRL IETARLTGAD VPVCVRSESC VMTGVGESLL PLDVPAMPAV 190 200 210 220 230 240 LVNPRVPVAT KDVFAALGLR KGQLNVGVND LIEAIVWPEA GAPVDDWLAM LSDGANDLEA 250 260 270 280 290 300 PAIRVQPVIG EVLARLRSTG ARLSRMSGSG ATCFAIFDDE AAAHHAAQTI RLDRPQWWVH VGTLGGGS

References

[1]

"Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255." Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J. Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed: 16517654] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nb-255 / ATCC 25391.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000115 Genomic DNA. Translation: ABA05846.1.
RefSeq	YP_319198.1. NC_007406.1.
3D structure databases
ProteinModelPortal	Q3SPE5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SPE5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3675022.
GenomeReviews	Gene locus Nwi_2593 in contig CP000115_GR.
KEGG	nwi:Nwi_2593.
NMPDR	fig|323098.3.peg.2515.
PATRIC	22702216. VBINitWin102302_2916.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	VSTAEVY.
PhylomeDB	Q3SPE5.
ProtClustDB	PRK14608.
Enzyme and pathway databases
BioCyc	NWIN323098:NWI_2593-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_NITWN

Accession

Primary (citable) accession number: Q3SPE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	October 11, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program