Reviewed, UniProtKB/Swiss-Prot Q3SNX3 (MASZ_NITWN)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	Nwi_2765

Organism

Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) [Complete proteome] [HAMAP]

Taxonomic identifier

323098 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter

Protein attributes

Sequence length	741 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 741	741	Malate synthase G HAMAP MF_00641		PRO_1000056914
Sites
	Active site	337	1	Proton acceptor By similarity
	Active site	628	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SNX3-1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 304959E432A852B2	FASTA	741	81,247
	10 20 30 40 50 60 MNRIDAHGLK IAPVLFDFIA GEATLRTGIK PDAFWAGLAA IVRDLGPRNR ELLAVRDRLQ 70 80 90 100 110 120 ARIDDWHRIH KDTPFDISAY TAFLTEIGYL EPEPETRQIE TANVDDEIGK ICGPQLVVPL 130 140 150 160 170 180 TNARYALNAA NARWGSLYDA LYGTDAIARE AGEAKGYDKA RGDKVIARAK AFLDMATPLA 190 200 210 220 230 240 NGAHADVAGY SVLNGRLSVT LRNGDTTGLK AETQFAGYQG DANAPSAILL VNHGLHIDIQ 250 260 270 280 290 300 IDRAHPIGRH YSAGVADVII EAAISTILDM EDSVAAVDAD DKVLVYRNAL GLMNGTLTDT 310 320 330 340 350 360 FEKSGKTLTR ALNPDRVYTA PDGGQLTLHG RSLLLIRNVG HHMFTDAVLD QRGAEIPEGL 370 380 390 400 410 420 LDAAISGLLA IHDINGVSKR RNSRSGSLYL VKPKMHGPDE VALTCELFAR VEAMLGLREN 430 440 450 460 470 480 TLKIGIMDEE RRTTVNLNAC IQTASKRVCF INTGFLDRTG DEIHTSMEAG PMIRKGEMKT 490 500 510 520 530 540 QPWIKAYEDW NVDIGLIEGL PGRAQIGKGM WAAPDRMADM LAQKINHPEA GATTAWVPSP 550 560 570 580 590 600 TAATLHALHY HQIDVRKRQQ ELKAGGRRAR LSDLLTIPVS HSNWPPEDVK QEIDNNCQGI 610 620 630 640 650 660 LGYVVRWIDQ GVGCSKVPDI HDVGLMEDRA TLRISSQHLA NWLHHGVVTH DQVMDSFKRM 670 680 690 700 710 720 AVIVDEQNAG DPLYKPMAPG FDSVAFKAAC DLVFRGREQP NGYTEHILTE RRREAKLMAQ 730 740 AARAFSGEAD TGSPRENATR Q

References

[1]

"Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255." Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J. Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed: 16517654] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000115 Genomic DNA. Translation: ABA06018.1.
RefSeq	YP_319370.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SNX3.
Genome annotation databases
GeneID	3675104.
GenomeReviews	Gene locus Nwi_2765 in contig CP000115_GR.
KEGG	nwi:Nwi_2765.
NMPDR	fig|323098.3.peg.2845.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3SNX3.
OMA	PKMHGPD.
Enzyme and pathway databases
BioCyc	NWIN323098:NWI_2765-MON.
Family and domain databases
HAMAP	MF_00641. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_NITWN

Accession

Primary (citable) accession number: Q3SNX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 11, 2005
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)