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Protein

Malate synthase G

Gene

glcB

Organism
Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei273Acetyl-CoAUniRule annotation1
Binding sitei310Acetyl-CoAUniRule annotation1
Active sitei337Proton acceptorUniRule annotation1
Binding sitei337GlyoxylateUniRule annotation1
Metal bindingi429MagnesiumUniRule annotation1
Binding sitei429GlyoxylateUniRule annotation1
Metal bindingi457MagnesiumUniRule annotation1
Binding sitei538Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei628Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciNWIN323098:G1G55-2835-MONOMER
UniPathwayiUPA00703; UER00720

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Nwi_2765
OrganismiNitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)
Taxonomic identifieri323098 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
Proteomesi
  • UP000002531 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000569141 – 741Malate synthase GAdd BLAST741

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei614Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi323098.Nwi_2765

Structurei

3D structure databases

ProteinModelPortaliQ3SNX3
SMRiQ3SNX3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 125Acetyl-CoA bindingUniRule annotation2
Regioni454 – 457Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3 Bacteria
COG2225 LUCA
HOGENOMiHOG000220740
KOiK01638
OMAiTEPVLHA
OrthoDBiPOG091H05P0

Family and domain databases

Gene3Di2.170.170.11, 1 hit
HAMAPiMF_00641 Malate_synth_G, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR023310 Malate_synth_G_beta_sub_dom
IPR001465 Malate_synthase
IPR006253 Malate_synthG
PANTHERiPTHR42739 PTHR42739, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01345 malate_syn_G, 1 hit

Sequencei

Sequence statusi: Complete.

Q3SNX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRIDAHGLK IAPVLFDFIA GEATLRTGIK PDAFWAGLAA IVRDLGPRNR
60 70 80 90 100
ELLAVRDRLQ ARIDDWHRIH KDTPFDISAY TAFLTEIGYL EPEPETRQIE
110 120 130 140 150
TANVDDEIGK ICGPQLVVPL TNARYALNAA NARWGSLYDA LYGTDAIARE
160 170 180 190 200
AGEAKGYDKA RGDKVIARAK AFLDMATPLA NGAHADVAGY SVLNGRLSVT
210 220 230 240 250
LRNGDTTGLK AETQFAGYQG DANAPSAILL VNHGLHIDIQ IDRAHPIGRH
260 270 280 290 300
YSAGVADVII EAAISTILDM EDSVAAVDAD DKVLVYRNAL GLMNGTLTDT
310 320 330 340 350
FEKSGKTLTR ALNPDRVYTA PDGGQLTLHG RSLLLIRNVG HHMFTDAVLD
360 370 380 390 400
QRGAEIPEGL LDAAISGLLA IHDINGVSKR RNSRSGSLYL VKPKMHGPDE
410 420 430 440 450
VALTCELFAR VEAMLGLREN TLKIGIMDEE RRTTVNLNAC IQTASKRVCF
460 470 480 490 500
INTGFLDRTG DEIHTSMEAG PMIRKGEMKT QPWIKAYEDW NVDIGLIEGL
510 520 530 540 550
PGRAQIGKGM WAAPDRMADM LAQKINHPEA GATTAWVPSP TAATLHALHY
560 570 580 590 600
HQIDVRKRQQ ELKAGGRRAR LSDLLTIPVS HSNWPPEDVK QEIDNNCQGI
610 620 630 640 650
LGYVVRWIDQ GVGCSKVPDI HDVGLMEDRA TLRISSQHLA NWLHHGVVTH
660 670 680 690 700
DQVMDSFKRM AVIVDEQNAG DPLYKPMAPG FDSVAFKAAC DLVFRGREQP
710 720 730 740
NGYTEHILTE RRREAKLMAQ AARAFSGEAD TGSPRENATR Q
Length:741
Mass (Da):81,247
Last modified:October 11, 2005 - v1
Checksum:i304959E432A852B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000115 Genomic DNA Translation: ABA06018.1
RefSeqiWP_011315963.1, NC_007406.1

Genome annotation databases

EnsemblBacteriaiABA06018; ABA06018; Nwi_2765
KEGGinwi:Nwi_2765

Similar proteinsi

Entry informationi

Entry nameiMASZ_NITWN
AccessioniPrimary (citable) accession number: Q3SNX3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 11, 2005
Last modified: April 25, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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