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Q3SNX3

- MASZ_NITWN

UniProt

Q3SNX3 - MASZ_NITWN

Protein

Malate synthase G

Gene

glcB

Organism
Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (11 Oct 2005)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei273 – 2731Acetyl-CoAUniRule annotation
    Binding sitei310 – 3101Acetyl-CoAUniRule annotation
    Active sitei337 – 3371Proton acceptorUniRule annotation
    Binding sitei337 – 3371GlyoxylateUniRule annotation
    Metal bindingi429 – 4291MagnesiumUniRule annotation
    Binding sitei429 – 4291GlyoxylateUniRule annotation
    Metal bindingi457 – 4571MagnesiumUniRule annotation
    Binding sitei538 – 5381Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei628 – 6281Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciNWIN323098:GJEG-2815-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:Nwi_2765
    OrganismiNitrobacter winogradskyi (strain Nb-255 / ATCC 25391)
    Taxonomic identifieri323098 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeNitrobacter
    ProteomesiUP000002531: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 741741Malate synthase GPRO_1000056914Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei614 – 6141Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi323098.Nwi_2765.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3SNX3.
    SMRiQ3SNX3. Positions 9-719.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
    Regioni454 – 4574Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiARTENVD.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q3SNX3-1 [UniParc]FASTAAdd to Basket

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    MNRIDAHGLK IAPVLFDFIA GEATLRTGIK PDAFWAGLAA IVRDLGPRNR    50
    ELLAVRDRLQ ARIDDWHRIH KDTPFDISAY TAFLTEIGYL EPEPETRQIE 100
    TANVDDEIGK ICGPQLVVPL TNARYALNAA NARWGSLYDA LYGTDAIARE 150
    AGEAKGYDKA RGDKVIARAK AFLDMATPLA NGAHADVAGY SVLNGRLSVT 200
    LRNGDTTGLK AETQFAGYQG DANAPSAILL VNHGLHIDIQ IDRAHPIGRH 250
    YSAGVADVII EAAISTILDM EDSVAAVDAD DKVLVYRNAL GLMNGTLTDT 300
    FEKSGKTLTR ALNPDRVYTA PDGGQLTLHG RSLLLIRNVG HHMFTDAVLD 350
    QRGAEIPEGL LDAAISGLLA IHDINGVSKR RNSRSGSLYL VKPKMHGPDE 400
    VALTCELFAR VEAMLGLREN TLKIGIMDEE RRTTVNLNAC IQTASKRVCF 450
    INTGFLDRTG DEIHTSMEAG PMIRKGEMKT QPWIKAYEDW NVDIGLIEGL 500
    PGRAQIGKGM WAAPDRMADM LAQKINHPEA GATTAWVPSP TAATLHALHY 550
    HQIDVRKRQQ ELKAGGRRAR LSDLLTIPVS HSNWPPEDVK QEIDNNCQGI 600
    LGYVVRWIDQ GVGCSKVPDI HDVGLMEDRA TLRISSQHLA NWLHHGVVTH 650
    DQVMDSFKRM AVIVDEQNAG DPLYKPMAPG FDSVAFKAAC DLVFRGREQP 700
    NGYTEHILTE RRREAKLMAQ AARAFSGEAD TGSPRENATR Q 741
    Length:741
    Mass (Da):81,247
    Last modified:October 11, 2005 - v1
    Checksum:i304959E432A852B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000115 Genomic DNA. Translation: ABA06018.1.
    RefSeqiYP_319370.1. NC_007406.1.

    Genome annotation databases

    EnsemblBacteriaiABA06018; ABA06018; Nwi_2765.
    GeneIDi3675104.
    KEGGinwi:Nwi_2765.
    PATRICi22702606. VBINitWin102302_3110.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000115 Genomic DNA. Translation: ABA06018.1 .
    RefSeqi YP_319370.1. NC_007406.1.

    3D structure databases

    ProteinModelPortali Q3SNX3.
    SMRi Q3SNX3. Positions 9-719.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 323098.Nwi_2765.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABA06018 ; ABA06018 ; Nwi_2765 .
    GeneIDi 3675104.
    KEGGi nwi:Nwi_2765.
    PATRICi 22702606. VBINitWin102302_3110.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi ARTENVD.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci NWIN323098:GJEG-2815-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255."
      Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J.
      Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Nb-255 / ATCC 25391.

    Entry informationi

    Entry nameiMASZ_NITWN
    AccessioniPrimary (citable) accession number: Q3SNX3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3