Q3SMZ4 (LPXD2_NITWN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase 2
EC=2.3.1.-

Gene names

Name:	lpxD2
Ordered Locus Names:	Nwi_3100

Organism

Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) [Complete proteome] [HAMAP]

Taxonomic identifier

323098 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Nitrobacter

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	UDP-3-O-acylglucosamine N-acyltransferase 2 HAMAP MF_00523		PRO_0000264401
Sites
	Active site	254	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SMZ4 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 6BA79AACF0D7CA50	FASTA	341	35,050
	10 20 30 40 50 60 MSGETFFLSN HSVAASQIAA LAGLSFVTED FAISAAGALA TAGIGEICYM DDTKYIEELK 70 80 90 100 110 120 VTRASACLIS PRFKELVPGG TFSFVTPHPY AIYAQVLALL YPEATIPASN FGTNGISSKA 130 140 150 160 170 180 NIHASAIVGH GVTIDPGASV GPNARIGGFT CIGSNAVIGP SVRIGRNCYI GANVTVAYAV 190 200 210 220 230 240 VGDRVIIHPG TSIGQDGFGF TFLGGKWVKV PQVGGVIIQD DVEVGANTTI DRGSMRATVI 250 260 270 280 290 300 GEGTKLDNLV QVAHNVTIGA HCVIAAQVGI AGSTTIGDFV AIGGHAGIAP HLTIGEKAQI 310 320 330 340 GGASGVMCDI PAGERWVGLP ARPSRAFFRQ FAALKRLAKK K

References

[1]

"Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255." Starkenburg S.R., Chain P.S.G., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J. Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed: 16517654] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nb-255 / ATCC 25391.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000115 Genomic DNA. Translation: ABA06347.1.
RefSeq	YP_319699.1. NC_007406.1.
3D structure databases
ProteinModelPortal	Q3SMZ4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SMZ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3676858.
GenomeReviews	Gene locus Nwi_3100 in contig CP000115_GR.
KEGG	nwi:Nwi_3100.
NMPDR	fig|323098.3.peg.3106.
PATRIC	22703402. VBINitWin102302_3504.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	VQIGRVI.
PhylomeDB	Q3SMZ4.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	NWIN323098:NWI_3100-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD2_NITWN

Accession

Primary (citable) accession number: Q3SMZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	October 11, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program