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Q3SMU7 (Q3SMU7_NITWN) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length813 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. RuleBase RU003363 HAMAP-Rule MF_01898

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. RuleBase RU003363 HAMAP-Rule MF_01898 SAAS SAAS013506

Cofactor

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ By similarity. HAMAP-Rule MF_01898

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01898

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01898.

Sequence similarities

Belongs to the type II topoisomerase family. HAMAP-Rule MF_01898 RuleBase RU000380

Contains 1 Toprim domain. HAMAP-Rule MF_01898

Contains Toprim domain. SAAS SAAS011557

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain433 – 548116Toprim By similarity HAMAP-Rule MF_01898

Sites

Metal binding4391Magnesium 1; catalytic By similarity HAMAP-Rule MF_01898
Metal binding5131Magnesium 1; catalytic By similarity HAMAP-Rule MF_01898
Metal binding5131Magnesium 2 By similarity HAMAP-Rule MF_01898
Metal binding5151Magnesium 2 By similarity HAMAP-Rule MF_01898
Site4641Interaction with DNA By similarity HAMAP-Rule MF_01898
Site4671Interaction with DNA By similarity HAMAP-Rule MF_01898

Sequences

Sequence LengthMass (Da)Tools
Q3SMU7 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: E2AC610DE795315D

FASTA81389,698
        10         20         30         40         50         60 
MNEPARQQIA DTESVAAIEY GAESIRVLKG LDAVRKRPGM YIGDTDDGSG LHHMVYEVVD 

        70         80         90        100        110        120 
NAIDEALAGH ATAVEVILNA DNSVTVRDDG RGIPVDIHKG EGVSAAEVIM TQLHAGGKFD 

       130        140        150        160        170        180 
QNSYKVSGGL HGVGVSVVNA LSSKLTLRIW RRDKEHLIEF ANGDALAPLK VVGDANGRRG 

       190        200        210        220        230        240 
TEVTFLASTE TFTNIEYDFA TLEHRLRELA FLNSGVNILL SDMRHAVERR EQMMYEGGVV 

       250        260        270        280        290        300 
EFVKYLDRNK KAMVADPIMV RAEMNGISVE AALWWNDSYH ENVLCFTNNI PQRDGGTHLA 

       310        320        330        340        350        360 
GFRGALTRQV NGYADAMAKK ERIALTGDDC REGLTAVLSV KVPDPKFSSQ TKDKLVSSEV 

       370        380        390        400        410        420 
RPVVENVINE ALAAWFEEHP TEAKLIVGKV VEAAAAREAA RKARELTRRK GALDVASLPG 

       430        440        450        460        470        480 
KLADCQERDP AKSELFIVEG DSAGGSAKQG RNREFQAVLP LRGKILNVER ARFDKMLSSE 

       490        500        510        520        530        540 
QIGTLITALG TGIGREDFDL SKLRYHKIIV MTDADVDGAH IRTLLLTFFF RQMPALIEAG 

       550        560        570        580        590        600 
HLYIAQPPLY KVTRGKSERY LKDERALEDY LIETGLDDCV YKLATGEDRG GRDLLALVDD 

       610        620        630        640        650        660 
ARVIRNVLQN LHGRYNRGVI EQAAIAGVMN PRITENLATA TAAADYIARR LDALADEVER 

       670        680        690        700        710        720 
GWVGRFVEGQ GFFFERTVRG VKDVAMIDDA LLGSADARKL DEYAGRLQEA YPRPGLLRRR 

       730        740        750        760        770        780 
DTETAIHGPV SLFEAVTDAG RKGVALQRYK GLGEMNPDQL WETTLDVSER SLLQVKIKEV 

       790        800        810 
DEADDIFTKL MGDVVEPRRE FIQDNSLSAN VDV 

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References

[1]"Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255."
Starkenburg S.R., Chain P.S., Sayavedra-Soto L.A., Hauser L., Land M.L., Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J., Hickey W.J.
Appl. Environ. Microbiol. 72:2050-2063(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nb-255 / ATCC 25391.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000115 Genomic DNA. Translation: ABA03272.1.
RefSeqYP_316624.1. NC_007406.1.

3D structure databases

ProteinModelPortalQ3SMU7.
SMRQ3SMU7. Positions 25-392.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323098.Nwi_0004.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA03272; ABA03272; Nwi_0004.
GeneID3676444.
KEGGnwi:Nwi_0004.
PATRIC22696297. VBINitWin102302_0005.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0187.
HOGENOMHOG000075155.
KOK02470.
OMAAFPTHYP.
OrthoDBEOG6P334W.

Enzyme and pathway databases

BioCycNWIN323098:GJEG-4-MONOMER.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 2 hits.
HAMAPMF_01898. GyrB.
InterProIPR002288. DNA_gyrase_B_C.
IPR011557. GyrB.
IPR003594. HATPase_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00418. TPI2FAMILY.
SMARTSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 2 hits.
TIGRFAMsTIGR01059. gyrB. 1 hit.
PROSITEPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ3SMU7_NITWN
AccessionPrimary (citable) accession number: Q3SMU7
Entry history
Integrated into UniProtKB/TrEMBL: October 11, 2005
Last sequence update: October 11, 2005
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)