Q3SM81 (UBIE_THIDA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquinone/menaquinone biosynthesis methyltransferase ubiE
EC=2.1.1.163
EC=2.1.1.201

Alternative name(s):
2-methoxy-6-polyprenyl-1,4-benzoquinol methylase
Demethylmenaquinone methyltransferase

Gene names

Name:	ubiE
Ordered Locus Names:	Tbd_0215

Organism

Thiobacillus denitrificans (strain ATCC 25259) [Complete proteome] [HAMAP]

Taxonomic identifier

292415 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Hydrogenophilales › Hydrogenophilaceae › Thiobacillus › Thiobacillus denitrificans

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Methyltransferase required for the conversion of demethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) By similarity. HAMAP-Rule MF_01813
Catalytic activity	A demethylmenaquinone + S-adenosyl-L-methionine = a menaquinol + S-adenosyl-L-homocysteine. HAMAP-Rule MF_01813 S-adenosyl-L-methionine + 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol = S-adenosyl-L-homocysteine + 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol. HAMAP-Rule MF_01813
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 8/8. HAMAP-Rule MF_01813 Cofactor biosynthesis; ubiquinone biosynthesis. HAMAP-Rule MF_01813
Sequence similarities	Belongs to the methyltransferase superfamily. UbiE family.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis Ubiquinone biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	aerobic respiration Inferred from electronic annotation. Source: HAMAP menaquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway ubiquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Ubiquinone/menaquinone biosynthesis methyltransferase ubiE HAMAP-Rule MF_01813		PRO_1000056313

Sequences

	Sequence		Length	Mass (Da)
	Q3SM81 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 5C0C9D545CFD24D1	FASTA	244	27,189
	10 20 30 40 50 60 MEKTTHFGYQ EVAESEKAAK VAEVFHSVAA QYDIMNDLMS AGLHRLWKRF AVAQAGLRPG 70 80 90 100 110 120 MKVLDVAGGT ADLTRLFLKE VGASGTVLLT DINFSMLREG RDRMLNEGRT PPAVQCDGER 130 140 150 160 170 180 LPFPDDYFDC VSVAFGLRNM THKDQALAEM HRVLRPGGRL LVLEFSKVWK PLEAAYDLYS 190 200 210 220 230 240 FKLLPLMGRL VANDAASYQY LAESIRMHPG QEELKAMMES AGFARVSYHN MTAGVVALHK GYKV

References

[1]

"The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans." Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W., Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P. J. Bacteriol. 188:1473-1488(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25259.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000116 Genomic DNA. Translation: AAZ96168.1.
RefSeq	YP_313973.1. NC_007404.1.
3D structure databases
ProteinModelPortal	Q3SM81.
ModBase	Search...
Protein-protein interaction databases
STRING	292415.Tbd_0215.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ96168; AAZ96168; Tbd_0215.
GeneID	3672862.
KEGG	tbd:Tbd_0215.
PATRIC	23966571. VBIThiDen82923_0217.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2226.
HOGENOM	HOG000249463.
KO	K03183.
OMA	PYQYLVE.
Enzyme and pathway databases
UniPathway	UPA00079; UER00169. UPA00232.
Family and domain databases
HAMAP	MF_01813. UbiE_methyltran.
InterPro	IPR004033. UbiE/COQ5_MeTrFase. IPR023576. UbiE/COQ5_MeTrFase_CS. IPR025765. UbiE/COQ5_MeTrFase_dom. [Graphical view]
PANTHER	PTHR10108:SF24. PTHR10108:SF24. 1 hit.
Pfam	PF01209. Ubie_methyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01934. MenG_MenH_UbiE. 1 hit.
PROSITE	PS01183. UBIE_1. 1 hit. PS01184. UBIE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBIE_THIDA

Accession

Primary (citable) accession number: Q3SM81

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 11, 2005
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program