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Q3SKU2 (NAGZ_THIDA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene names
Name:nagZ
Ordered Locus Names:Tbd_0727
OrganismThiobacillus denitrificans (strain ATCC 25259) [Complete proteome] [HAMAP]
Taxonomic identifier292415 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaHydrogenophilalesHydrogenophilaceaeThiobacillus

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides By similarity. HAMAP-Rule MF_00364

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364

Pathway

Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00364.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 359359Beta-hexosaminidase HAMAP-Rule MF_00364
PRO_0000234926

Regions

Region168 – 1692Substrate binding By similarity

Sites

Active site1811Proton donor/acceptor By similarity
Active site2521Nucleophile By similarity
Binding site641Substrate By similarity
Binding site721Substrate By similarity
Binding site1381Substrate By similarity
Site1791Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3SKU2 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: DDBA63811D00A9C8

FASTA35938,792
        10         20         30         40         50         60 
MTLGPLMLDV AGTELCDDDR RRLSDPLVGG VILFSRNYRD PAQLAALCEE IHALKAAPLL 

        70         80         90        100        110        120 
IGVDHEGGRV QRFRDGFTRL PPMRSLGAIW NEHPQRAREL ARETGYVLAS ELRAHGVDFS 

       130        140        150        160        170        180 
FAPVLDLDYG ASSVIGDRAF HADPQAVFQL GQAVMLGMKD AGMAACGKHF PGHGFVIADS 

       190        200        210        220        230        240 
HVDIPVDRRT LGDIARADLV PFRLMVEAGL AAMMPAHVIY PEVDAQPAGF SRVWLQKILR 

       250        260        270        280        290        300 
QQLGFDGAVF SDDLCMAGAA FAGGIVERVR AALDAGCDMA LVCNHPELAD EVLAKLDVDW 

       310        320        330        340        350 
PAPARARLAR MHGHPAHAPT MTALRESARY AEALHHVAGL GRDSAELDLS DPTDYCGRA 

« Hide

References

[1]"The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans."
Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W., Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P.
J. Bacteriol. 188:1473-1488(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25259.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000116 Genomic DNA. Translation: AAZ96680.1.
RefSeqYP_314485.1. NC_007404.1.

3D structure databases

ProteinModelPortalQ3SKU2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING292415.Tbd_0727.

Protein family/group databases

CAZyGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ96680; AAZ96680; Tbd_0727.
GeneID3673818.
KEGGtbd:Tbd_0727.
PATRIC23967627. VBIThiDen82923_0728.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000248526.
KOK01207.
OMADSHVDIP.
OrthoDBEOG6BCT06.

Enzyme and pathway databases

BioCycTDEN292415:GHWG-748-MONOMER.
UniPathwayUPA00544.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
HAMAPMF_00364. NagZ.
InterProIPR022956. Beta_hexosaminidase_bac.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNAGZ_THIDA
AccessionPrimary (citable) accession number: Q3SKU2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: October 11, 2005
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries