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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Thiobacillus denitrificans (strain ATCC 25259)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nucleotidyltransferase, Transferase
LigandMagnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:Tbd_0783
OrganismiThiobacillus denitrificans (strain ATCC 25259)
Taxonomic identifieri292415 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesThiobacillaceaeThiobacillus
Proteomesi
  • UP000008291 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002316951 – 850Bifunctional uridylyltransferase/uridylyl-removing enzymeAdd BLAST850

Interactioni

Protein-protein interaction databases

STRINGi292415.Tbd_0783.

Structurei

3D structure databases

ProteinModelPortaliQ3SKP1.
SMRiQ3SKP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini436 – 558HDPROSITE-ProRule annotationAdd BLAST123
Domaini674 – 755ACT 1UniRule annotationAdd BLAST82
Domaini783 – 850ACT 2UniRule annotationAdd BLAST68

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 317UridylyltransferaseAdd BLAST317
Regioni318 – 673Uridylyl-removingAdd BLAST356

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105E1P. Bacteria.
COG2844. LUCA.
HOGENOMiHOG000261778.
KOiK00990.
OMAiARICGYF.
OrthoDBiPOG091H04HO.

Family and domain databases

CDDicd00077. HDc. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf. 1 hit.
InterProiView protein in InterPro
IPR002912. ACT_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR002934. Polymerase_NTP_transf_dom.
IPR010043. UTase/UR.
PANTHERiPTHR13734:SF52. PTHR13734:SF52. 1 hit.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiView protein in SMART
SM00471. HDc. 1 hit.
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiView protein in PROSITE
PS51671. ACT. 2 hits.
PS51831. HD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3SKP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSARPFADLR ERLKSGRASL AAAYREKPRA AHYLARHAAL VDALLAELST
60 70 80 90 100
RLGLARGICL VAVGGYGRGE LFPGSDVDVM LLLPAEPLEA ERQALESWVQ
110 120 130 140 150
ACWDVGLEIG HSVRTVDACL AEADADITVE TNLLEARRVW GATALFDDFG
160 170 180 190 200
RRFRARFDAQ RFFDGKLAEQ HARHARFDDS AYKLEPNLKD SPGGLRDLHT
210 220 230 240 250
IHWLAQACDI DAGWSGIARA GLLTEGEARR VAREERWLAK LRIHLHLLAG
260 270 280 290 300
RREDRLAFDY QSELAACLGL APTAHRRAGE RLMQGYFRAA KLVQRANDIL
310 320 330 340 350
IQSLRVRLFP VVAPPLPIDD DFQLRAGLLE ARDPDVFLRK PDALLRAFLV
360 370 380 390 400
YARHPQLAGF EPTTLRAVWR ASARVDRAFR ATPAHRALFI ALLREPLGVT
410 420 430 440 450
RALRAMHRYG LLGRYIPAFG RIVGQMQHDL FHVYTVDEHI LTVLRNLRRF
460 470 480 490 500
TVAQLAHEFP LASRLIAAFD KPELLYLAAL FHDIAKGRGG DHSALGALDA
510 520 530 540 550
RGFCRQHGLD KTDTDLVAWL VDMHLVMSRT SQKEDISDPK VIAAFAARVG
560 570 580 590 600
DTRRLDALYL LTVADIRGTS PTVWNAWKGK LLEDLYHAAF ARLQGADLAI
610 620 630 640 650
AGIAARREEA RVNLALYGLP RDAADALWRH LDKAYFARFD ARDMAWHARM
660 670 680 690 700
LWRRDATAGA VVRARLSPAG EGIQVMVYAP DRPDIFVRIC AFFARIQYTV
710 720 730 740 750
LEAKIHTTRN GYALDSFQVM DLAHRNIHYR DFLAFVEYEL ARDLDPARPL
760 770 780 790 800
QAVQPGRLSR HQRHHPYPAA VHLEADRGGD GQVLSITCAD RGGLLFAIAE
810 820 830 840 850
ELMRHEISVY AAKIDTLGER VEDTFLIRGE RLNAPPERAA LENELRGVLG
Length:850
Mass (Da):95,305
Last modified:October 11, 2005 - v1
Checksum:iAA673373E9F73EE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000116 Genomic DNA. Translation: AAZ96736.1.
RefSeqiWP_011311295.1. NC_007404.1.

Genome annotation databases

EnsemblBacteriaiAAZ96736; AAZ96736; Tbd_0783.
KEGGitbd:Tbd_0783.

Similar proteinsi

Entry informationi

Entry nameiGLND_THIDA
AccessioniPrimary (citable) accession number: Q3SKP1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 11, 2005
Last modified: September 27, 2017
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families