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Q3SKP1

- GLND_THIDA

UniProt

Q3SKP1 - GLND_THIDA

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Thiobacillus denitrificans (strain ATCC 25259)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciTDEN292415:GHWG-804-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:Tbd_0783
OrganismiThiobacillus denitrificans (strain ATCC 25259)
Taxonomic identifieri292415 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaHydrogenophilalesHydrogenophilaceaeThiobacillus
ProteomesiUP000008291: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 850850Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000231695Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi292415.Tbd_0783.

Structurei

3D structure databases

ProteinModelPortaliQ3SKP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini437 – 546110HDUniRule annotationAdd
BLAST
Domaini674 – 75582ACT 1UniRule annotationAdd
BLAST
Domaini783 – 85068ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 317317UridylyltransferaseAdd
BLAST
Regioni318 – 673356Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiLYCLWDM.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3SKP1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSARPFADLR ERLKSGRASL AAAYREKPRA AHYLARHAAL VDALLAELST
60 70 80 90 100
RLGLARGICL VAVGGYGRGE LFPGSDVDVM LLLPAEPLEA ERQALESWVQ
110 120 130 140 150
ACWDVGLEIG HSVRTVDACL AEADADITVE TNLLEARRVW GATALFDDFG
160 170 180 190 200
RRFRARFDAQ RFFDGKLAEQ HARHARFDDS AYKLEPNLKD SPGGLRDLHT
210 220 230 240 250
IHWLAQACDI DAGWSGIARA GLLTEGEARR VAREERWLAK LRIHLHLLAG
260 270 280 290 300
RREDRLAFDY QSELAACLGL APTAHRRAGE RLMQGYFRAA KLVQRANDIL
310 320 330 340 350
IQSLRVRLFP VVAPPLPIDD DFQLRAGLLE ARDPDVFLRK PDALLRAFLV
360 370 380 390 400
YARHPQLAGF EPTTLRAVWR ASARVDRAFR ATPAHRALFI ALLREPLGVT
410 420 430 440 450
RALRAMHRYG LLGRYIPAFG RIVGQMQHDL FHVYTVDEHI LTVLRNLRRF
460 470 480 490 500
TVAQLAHEFP LASRLIAAFD KPELLYLAAL FHDIAKGRGG DHSALGALDA
510 520 530 540 550
RGFCRQHGLD KTDTDLVAWL VDMHLVMSRT SQKEDISDPK VIAAFAARVG
560 570 580 590 600
DTRRLDALYL LTVADIRGTS PTVWNAWKGK LLEDLYHAAF ARLQGADLAI
610 620 630 640 650
AGIAARREEA RVNLALYGLP RDAADALWRH LDKAYFARFD ARDMAWHARM
660 670 680 690 700
LWRRDATAGA VVRARLSPAG EGIQVMVYAP DRPDIFVRIC AFFARIQYTV
710 720 730 740 750
LEAKIHTTRN GYALDSFQVM DLAHRNIHYR DFLAFVEYEL ARDLDPARPL
760 770 780 790 800
QAVQPGRLSR HQRHHPYPAA VHLEADRGGD GQVLSITCAD RGGLLFAIAE
810 820 830 840 850
ELMRHEISVY AAKIDTLGER VEDTFLIRGE RLNAPPERAA LENELRGVLG
Length:850
Mass (Da):95,305
Last modified:October 11, 2005 - v1
Checksum:iAA673373E9F73EE8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000116 Genomic DNA. Translation: AAZ96736.1.
RefSeqiWP_011311295.1. NC_007404.1.
YP_314541.1. NC_007404.1.

Genome annotation databases

EnsemblBacteriaiAAZ96736; AAZ96736; Tbd_0783.
GeneIDi3673977.
KEGGitbd:Tbd_0783.
PATRICi23967735. VBIThiDen82923_0782.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000116 Genomic DNA. Translation: AAZ96736.1 .
RefSeqi WP_011311295.1. NC_007404.1.
YP_314541.1. NC_007404.1.

3D structure databases

ProteinModelPortali Q3SKP1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 292415.Tbd_0783.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ96736 ; AAZ96736 ; Tbd_0783 .
GeneIDi 3673977.
KEGGi tbd:Tbd_0783.
PATRICi 23967735. VBIThiDen82923_0782.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi LYCLWDM.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci TDEN292415:GHWG-804-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans."
    Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W., Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P.
    J. Bacteriol. 188:1473-1488(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25259.

Entry informationi

Entry nameiGLND_THIDA
AccessioniPrimary (citable) accession number: Q3SKP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 11, 2005
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3