Q3SKN1 (LPXD_THIDA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	Tbd_0795

Organism

Thiobacillus denitrificans (strain ATCC 25259) [Complete proteome] [HAMAP]

Taxonomic identifier

292415 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Hydrogenophilales › Hydrogenophilaceae › Thiobacillus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000264450
Sites
	Active site	238	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SKN1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 561C003C4DD58C42	FASTA	343	35,725
	10 20 30 40 50 60 MAVTLAALVE RFGGELVGDS ARSVRQVAPL DRACADDIGF VSQSKYLAEL ATTGAGAVIL 70 80 90 100 110 120 PPDARDATDL PRILTPNPYL YFARVSALLN PPPRPAAGVH PAATVAADAE VAADASIAAG 130 140 150 160 170 180 AVIGSGARIG ARSVIGANSV VGDGARVGED CLLHANVSLY HGCQVGDRVI LHAGCVIGAD 190 200 210 220 230 240 GFGFAPNEGR WEKIPQIGRV LIGDDVEVGA CTTIDRGALE DTVIEEGVKL DNLIQVAHNV 250 260 270 280 290 300 VIGAHSAIAA CTGIAGSAKI GRHCTIGGAA MIFGHIEIAD GTRISTNTLI TKSLPKRGTY 310 320 330 340 TSALPFSEHE VWQKNAVHMR NLDKLVTRVK QLEKRLNELE NKT

References

[1]

"The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans." Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W., Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P. J. Bacteriol. 188:1473-1488(2006) [PubMed: 16452431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25259.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000116 Genomic DNA. Translation: AAZ96748.1.
RefSeq	YP_314553.1. NC_007404.1.
3D structure databases
ProteinModelPortal	Q3SKN1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SKN1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3673492.
GenomeReviews	Gene locus Tbd_0795 in contig CP000116_GR.
KEGG	tbd:Tbd_0795.
NMPDR	fig|292415.3.peg.102.
PATRIC	23967759. VBIThiDen82923_0794.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	CFVGKNT.
PhylomeDB	Q3SKN1.
Enzyme and pathway databases
BioCyc	TDEN292415:TBD_0795-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_THIDA

Accession

Primary (citable) accession number: Q3SKN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	October 11, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program