Q3SKM9 (LPXA_THIDA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
Short name=UDP-N-acetylglucosamine acyltransferase
EC=2.3.1.129

Gene names

Name:	lpxA
Ordered Locus Names:	Tbd_0797

Organism

Thiobacillus denitrificans (strain ATCC 25259) [Complete proteome] [HAMAP]

Taxonomic identifier

292415 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Hydrogenophilales › Hydrogenophilaceae › Thiobacillus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00387
Catalytic activity	(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetylglucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetylglucosamine. HAMAP MF_00387
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6. HAMAP MF_00387
Subunit structure	Homotrimer By similarity. HAMAP MF_00387
Subcellular location	Cytoplasm By similarity HAMAP MF_00387.
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxA subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase HAMAP MF_00387		PRO_1000080215

Sequences

	Sequence		Length	Mass (Da)
	Q3SKM9 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: A84DA98438312487	FASTA	258	27,706
	10 20 30 40 50 60 MATIHPTALV APGARLADDV EIGPYSVIGE HVEIGAGTTV GAHAVLTGHT TIGERNKIFH 70 80 90 100 110 120 FVSLGEAPQD KKYAGEPTRL EIGDYNVIRE FCTFNIGTVQ DRGVTRIGHH NWIMAYVHIA 130 140 150 160 170 180 HDCVVGDRTI FANNASLAGH AEVGDWAILG GFTGVHQFCK VGAHVMTGIS SVVFKDIPPF 190 200 210 220 230 240 VMASGQPAAP HGLNNEGLKR RGFSAEALSA LKRAYKILYR EGNTLAEAQA KLAPEAAKHA 250 EVQQLLDFLA RAERGIIR

References

[1]

"The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans." Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W., Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P. J. Bacteriol. 188:1473-1488(2006) [PubMed: 16452431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25259.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000116 Genomic DNA. Translation: AAZ96750.1.
RefSeq	YP_314555.1. NC_007404.1.
3D structure databases
ProteinModelPortal	Q3SKM9.
SMR	Q3SKM9. Positions 2-258.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SKM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3673494.
GenomeReviews	Gene locus Tbd_0797 in contig CP000116_GR.
KEGG	tbd:Tbd_0797.
NMPDR	fig|292415.3.peg.104.
PATRIC	23967763. VBIThiDen82923_0796.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1043.
HOGENOM	HBG659295.
OMA	REFCTFN.
PhylomeDB	Q3SKM9.
ProtClustDB	PRK05289.
Enzyme and pathway databases
BioCyc	TDEN292415:TBD_0797-MONOMER.
Family and domain databases
HAMAP	MF_00387. LpxA. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR010137. Lipid_A_lpxA. IPR011004. Trimer_LpxA-like. [Graphical view]
KO	K00677.
Pfam	PF00132. Hexapep. 4 hits. [Graphical view]
PIRSF	PIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01852. Lipid_A_lpxA. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXA_THIDA

Accession

Primary (citable) accession number: Q3SKM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 11, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program