Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Thiobacillus denitrificans (strain ATCC 25259)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciTDEN292415:G1G56-816-MONOMER
UniPathwayiUPA00359; UER00481

Protein family/group databases

CAZyiGT19 Glycosyltransferase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:Tbd_0798
OrganismiThiobacillus denitrificans (strain ATCC 25259)
Taxonomic identifieri292415 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesThiobacillaceaeThiobacillus
Proteomesi
  • UP000008291 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002552291 – 372Lipid-A-disaccharide synthaseAdd BLAST372

Interactioni

Protein-protein interaction databases

STRINGi292415.Tbd_0798

Structurei

3D structure databases

ProteinModelPortaliQ3SKM8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D0V Bacteria
COG0763 LUCA
HOGENOMiHOG000018003
KOiK00748
OMAiPTVWAWR
OrthoDBiPOG091H01NW

Family and domain databases

HAMAPiMF_00392 LpxB, 1 hit
InterProiView protein in InterPro
IPR003835 Glyco_trans_19
PANTHERiPTHR30372 PTHR30372, 1 hit
PfamiView protein in Pfam
PF02684 LpxB, 1 hit
TIGRFAMsiTIGR00215 lpxB, 1 hit

Sequencei

Sequence statusi: Complete.

Q3SKM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAGEASGDL LGAHFFDALK KNRPGLTAAG IAGPRMVEAG VKAIYPSEKL
60 70 80 90 100
AVNGYVEVLR HLPELLWIRA RITRHFLRER PRVFVGIDAP DFNFTLEAAL
110 120 130 140 150
KRAGVPTIHF VSPSIWAWRP ERIERIKQAV SHMLVVFPFE EAIYRDAGIP
160 170 180 190 200
VSYVGHPLAD VIPLQAPTGA ARATLGLGDG PIVALLPGSR LSEVDRHARL
210 220 230 240 250
MLEAAMQVRA KEMDVRFVLP AASEAARERI ARAAQGLDLP LTVLAGRSHQ
260 270 280 290 300
ALAACDVAVV ASGTATLEAA LFKKPMVITY RVPALTARLM RKKALLPWIG
310 320 330 340 350
LPNILARDFV VPERVQEAAT PDALAADVLA WLGDAARRAA LAVTFDALHR
360 370
DLRQGASARI AAAIAPYLEA AR
Length:372
Mass (Da):40,189
Last modified:October 11, 2005 - v1
Checksum:i79246C5A6014C2CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000116 Genomic DNA Translation: AAZ96751.1
RefSeqiWP_011311310.1, NC_007404.1

Genome annotation databases

EnsemblBacteriaiAAZ96751; AAZ96751; Tbd_0798
KEGGitbd:Tbd_0798

Similar proteinsi

Entry informationi

Entry nameiLPXB_THIDA
AccessioniPrimary (citable) accession number: Q3SKM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 11, 2005
Last modified: February 28, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health