Q3SK89 (Q3SK89_THIDA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A HAMAP-Rule MF_01897
EC=5.99.1.3 HAMAP-Rule MF_01897

Gene names

Name:	gyrA HAMAP-Rule MF_01897
Ordered Locus Names:	Tbd_0948

Organism

Thiobacillus denitrificans (strain ATCC 25259) [Complete proteome] [HAMAP] EMBL AAZ96901.1

Taxonomic identifier

292415 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Hydrogenophilales › Hydrogenophilaceae › Thiobacillus › Thiobacillus denitrificans

Protein attributes

Sequence length	849 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897 SAAS SAAS005743
Subunit structure	Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01897
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP-Rule MF_01897

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP-Rule MF_01897
Ligand	ATP-binding SAAS SAAS013760 HAMAP-Rule MF_01897 DNA-binding SAAS SAAS005743 HAMAP-Rule MF_01897 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005743 HAMAP-Rule MF_01897
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	122	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity HAMAP-Rule MF_01897

Sequences

	Sequence		Length	Mass (Da)
	Q3SK89 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 1FF49E590121D7E4	FASTA	849	94,416
	10 20 30 40 50 60 MEQFAKETLP VSLEEEMRRS YLDYAMSVIV GRALPDVRDG LKPVHRRVLY AMNELGNDWN 70 80 90 100 110 120 KAYKKSARVV GDVIGKYHPH GDTAVYDTMV RMAQDFSLRY PLIDGQGNFG SVDGDNAAAM 130 140 150 160 170 180 RYTEVRMARI AHELLADLDK ETVDFGPNYD GSEQEPLVLP TKIPNLLING SSGIAVGMAT 190 200 210 220 230 240 NIPPHNLTDI CNALFALLDA PATDIESLIS IVQAPDFPTA GIIYGLSGVR DGYRTGRGRV 250 260 270 280 290 300 VMRATCHVED LDKSGGKQAI IIDELPYQVN KANLLIRIGE LVRDKQIDGI ADLRDESDKS 310 320 330 340 350 360 GMRVYIELKR GENADVVLNN LYKQTQMQDT FGMNMVALID GQPRLLNLKE MLDAFLRHRR 370 380 390 400 410 420 EVVTRRTVFD LRKARERGHI LEGLAVALAN VDEIVELIKS SETPAIAKER LLGRAWKSAM 430 440 450 460 470 480 VEEMLARIDP EYSRPVNMGP EFGLHADGYH LSEIQAQRIL EMQLQRLTNL ESDKILAEYK 490 500 510 520 530 540 EIMAKIADLL DILANPARIT QIIREELTQV RDQFGDGRRS TIVENAQDMS MEDLIKPEEM 550 560 570 580 590 600 VVTLSHGGYM KSQPLDDYRA QKRGGRGKQA AGTKDEDFIE KMFVANTHDY ILCFSNRGRL 610 620 630 640 650 660 YWLKVYNVPQ GGRAARGKPI VNLLPLEDGE KISALLPVKT FDDEHYVFMA TANGIVKKTP 670 680 690 700 710 720 LSEFSRPRTA GIIAVGLDDG DVLCGASITD GRHDVMLFSD SGKAVRFDEN DVRPMGRAAR 730 740 750 760 770 780 GVIGMKLAKG AKLISLLVAE DENDFVLTAT ENGYGKRTPI AEYTRHARAT QGMIAIQTSE 790 800 810 820 830 840 RNGKVVAANL VKADDEIMLI TTGGVLIRTR VKEIRAMSRA TQGVTLINLD KGEKLISLEK VAETDNEEE

References

[1]

"The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans." Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W., Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P. J. Bacteriol. 188:1473-1488(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25259.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000116 Genomic DNA. Translation: AAZ96901.1.
RefSeq	YP_314706.1. NC_007404.1.
3D structure databases
ProteinModelPortal	Q3SK89.
SMR	Q3SK89. Positions 30-522, 537-843.
ModBase	Search...
Protein-protein interaction databases
STRING	292415.Tbd_0948.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ96901; AAZ96901; Tbd_0948.
GeneID	3672740.
KEGG	tbd:Tbd_0948.
PATRIC	23968039. VBIThiDen82923_0933.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0188.
HOGENOM	HOG000076278.
KO	K02469.
OMA	IAQEDVV.
ProtClustDB	PRK05560.
Family and domain databases
Gene3D	1.10.268.10. 1 hit. 3.30.1360.40. 1 hit. 3.90.199.10. 1 hit.
HAMAP	MF_01897. GyrA.
InterPro	IPR024946. Arg_repress_C-like. IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_like_dom. [Graphical view]
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q3SK89_THIDA

Accession

Primary (citable) accession number: Q3SK89

Entry history

Integrated into UniProtKB/TrEMBL:	October 11, 2005
Last sequence update:	October 11, 2005
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)