Q3SK83 (AROA_THIDA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	Tbd_0954

Organism

Thiobacillus denitrificans (strain ATCC 25259) [Complete proteome] [HAMAP]

Taxonomic identifier

292415 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Hydrogenophilales › Hydrogenophilaceae › Thiobacillus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000012503

Sequences

	Sequence		Length	Mass (Da)
	Q3SK83 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 43D45A5FDAF24718	FASTA	428	45,482
	10 20 30 40 50 60 MEFLDLPGRS RASGTVRLPG SKSISNRVLL LAALAEGVTD VYDVLDSDDT RYMLAALQAL 70 80 90 100 110 120 GVGVEDRGDN HWRVTGVAGA FPARQAELFL GNAGTAFRPL TAVLALSGGD YVLDGVARMH 130 140 150 160 170 180 ERPIGDLVDA LRQLGGRIDY RGTLGFPPLH VHPPQAGADV AHIRGNVSSQ FLTGLLMALP 190 200 210 220 230 240 LRRLRTRVEV VGELISKPYI EITLAMLRRF GVEVARDGWQ AFSVAADACY RSPREIYVEG 250 260 270 280 290 300 DASSASYFLA AGAIGGGPVR VEGVGRDSVQ GDVGFADALA AMGARIAMGP NWIEACAPAQ 310 320 330 340 350 360 GRLKAIDLDC NAIPDAAMTL AVAALFADGT TTLRNIASWR VKETDRIAAM ATELRKVGAT 370 380 390 400 410 420 VEEGDDFLRV TPPETLRAGA VIDTYDDHRM AMCLSLVSLG GVAVRINDPG CVAKTFPGYF NAFAEIAR

References

[1]

"The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans." Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W., Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P. J. Bacteriol. 188:1473-1488(2006) [PubMed: 16452431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25259.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000116 Genomic DNA. Translation: AAZ96907.1.
RefSeq	YP_314712.1. NC_007404.1.
3D structure databases
HSSP	HSSP built from PDB template 2AA9 based on UniProtKB P0A6D3.
ProteinModelPortal	Q3SK83.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SK83.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3672746.
GenomeReviews	Gene locus Tbd_0954 in contig CP000116_GR.
KEGG	tbd:Tbd_0954.
NMPDR	fig|292415.3.peg.544.
PATRIC	23968051. VBIThiDen82923_0939.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0128.
HOGENOM	HBG646626.
OMA	GADIEWG.
PhylomeDB	Q3SK83.
Enzyme and pathway databases
BioCyc	TDEN292415:TBD_0954-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_THIDA

Accession

Primary (citable) accession number: Q3SK83

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 11, 2005
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program