Q3SK38 (ISPD_THIDA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	Tbd_1003

Organism

Thiobacillus denitrificans (strain ATCC 25259) [Complete proteome] [HAMAP]

Taxonomic identifier

292415 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Hydrogenophilales › Hydrogenophilaceae › Thiobacillus

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_0000237834
Sites
	Site	15	1	Transition state stabilizer By similarity
	Site	22	1	Transition state stabilizer By similarity
	Site	161	1	Positions MEP for the nucleophilic attack By similarity
	Site	214	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SK38 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: DF345F47DA4B5743	FASTA	233	25,286
	10 20 30 40 50 60 MKFYALIPAA GSGSRMGGAI EKQYMDVNSV PMIAHAMMVL AREPRIARIF VVLSPTDKRW 70 80 90 100 110 120 NNYEWQGWEE RIEVLRCGGG TRAETVLNAL DAIAEVCDPA DWVLVHDAAR PCLPDEMLGK 130 140 150 160 170 180 LLDEVADDPV GGLLAVPVAD TLKRAAGDTS SGTRVEATVP RAGLWQAQTP QMFRHGTLTE 190 200 210 220 230 ALRAAGSDMT DEASAIEKLG LQPQLVESDS RNLKVTYPQD LELASLILGK MNA

References

[1]

"The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans." Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W., Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P. J. Bacteriol. 188:1473-1488(2006) [PubMed: 16452431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25259.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000116 Genomic DNA. Translation: AAZ96956.1.
RefSeq	YP_314761.1. NC_007404.1.
3D structure databases
ProteinModelPortal	Q3SK38.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SK38.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3671226.
GenomeReviews	Gene locus Tbd_1003 in contig CP000116_GR.
KEGG	tbd:Tbd_1003.
NMPDR	fig|292415.3.peg.733.
PATRIC	23968153. VBIThiDen82923_0988.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	ALSIVHT.
PhylomeDB	Q3SK38.
Enzyme and pathway databases
BioCyc	TDEN292415:TBD_1003-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_THIDA

Accession

Primary (citable) accession number: Q3SK38

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 11, 2005
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program