Q3SK34 (SYT_THIDA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	Tbd_1007

Organism

Thiobacillus denitrificans (strain ATCC 25259) [Complete proteome] [HAMAP]

Taxonomic identifier

292415 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Hydrogenophilales › Hydrogenophilaceae › Thiobacillus

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 637	637	Threonine--tRNA ligase HAMAP MF_00184		PRO_1000020547
Regions
	Region	244 – 535	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	335	1	Zinc; catalytic By similarity
	Metal binding	386	1	Zinc; catalytic By similarity
	Metal binding	512	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SK34 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 38B90F70D31E6CB3	FASTA	637	72,180
	10 20 30 40 50 60 MVTVTLPDGS VRPFEGPVTV AEVASSIGAG LAKAALAGKV DGKLVDTSYV IDADAQLAIV 70 80 90 100 110 120 TAKDAEALDL IRHDAAHVMA QAVQELYPGT QVTIGPAIED GFYYDFAREQ PFTPEDLEKI 130 140 150 160 170 180 EKRMDEIVKR DLPIRREVWS RDEAMKVFGD LGETYKVQII DEVIPKGEEL SIYRQGEWFD 190 200 210 220 230 240 VCRGPHLPST GKLPRAFKLM KLAGAYWRGD SKNAMLQRIY GTAWAKKEDL EAYLHRLEEA 250 260 270 280 290 300 EKRDHRRLAK QLDLLHMQDE APGMVFWHPK GWIVWQQIEQ YMREKFVEYG YQEVRTPAVM 310 320 330 340 350 360 DRSMWEKSGH WENYRDNMFT TASENRDYAV KPMNCPGHVQ IFNSGLHSYR DLPLRLAEFG 370 380 390 400 410 420 SCHRNEPSGA LHGIMRVRGF TQDDAHIFCM EEQVEQEVAD FIVMLQKVYA DFGFNDVLVK 430 440 450 460 470 480 LSTRPDKRVG SDESWDKAES ALAAALEKNG LSFDLQPGEG AFYGPKIEFT LKDTLGRLWQ 490 500 510 520 530 540 CGTIQLDFNL PVRLGAEFVA EDNTRKIPVM LHRAILGSME RFIGILIEHH AGNFPLWLAP 550 560 570 580 590 600 VQVMVMNISE RQAAYAEAVA EALRRAGIRA ALDLSNNKIN YKIREHSLQK LPYQLVVGDK 610 620 630 EMEARVVAVR ARGNQDMGQL GLDDLIARLR AEVLARQ

References

[1]

"The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans." Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W., Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P. J. Bacteriol. 188:1473-1488(2006) [PubMed: 16452431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25259.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000116 Genomic DNA. Translation: AAZ96960.1.
RefSeq	YP_314765.1. NC_007404.1.
3D structure databases
ProteinModelPortal	Q3SK34.
SMR	Q3SK34. Positions 243-636.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3SK34.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3671231.
GenomeReviews	Gene locus Tbd_1007 in contig CP000116_GR.
KEGG	tbd:Tbd_1007.
NMPDR	fig|292415.3.peg.737.
PATRIC	23968163. VBIThiDen82923_0992.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0441.
HOGENOM	HBG352811.
OMA	VGSDALW.
PhylomeDB	Q3SK34.
Enzyme and pathway databases
BioCyc	TDEN292415:TBD_1007-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_THIDA

Accession

Primary (citable) accession number: Q3SK34

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 11, 2005
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program