Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Thiobacillus denitrificans (strain ATCC 25259)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei253ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Tbd_1734
OrganismiThiobacillus denitrificans (strain ATCC 25259)
Taxonomic identifieri292415 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesThiobacillaceaeThiobacillus
Proteomesi
  • UP000008291 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002374161 – 472Glutamate--tRNA ligaseAdd BLAST472

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi292415.Tbd_1734

Structurei

3D structure databases

ProteinModelPortaliQ3SI46
SMRiQ3SI46
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi18 – 28"HIGH" regionUniRule annotationAdd BLAST11
Motifi250 – 254"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiAYYAFDT
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.8.702 hits
3.40.50.6201 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020752 aa-tRNA-synth_I_codon-bd_sub1
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q3SI46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPFSRPHHP MIRTRFAPSP TGYLHIGGAR TALFSWAFAR HHGGQFVLRI
60 70 80 90 100
EDTDIARSTP EAVQAILDGM AWLDLNHDEG PFYQTQRLYR YKEVIEQMLA
110 120 130 140 150
SGDAYHCYCS TEELDEMREA QRARGEKPRY DGRWRPEPGK TLPVPPSGVQ
160 170 180 190 200
PVVRFRNPTD GTVAWKDLVK GTIEFSNAEL DDLIIARADG TPTYNFCVVV
210 220 230 240 250
DDWDMRITHV IRGDDHVNNT PRQINILKAL GATVPEYAHL SMILGDDGTK
260 270 280 290 300
LSKRHGAVSV MQYFEEGYLP EAVINYLARL GWSHGDAELF DREQFVEWFD
310 320 330 340 350
LDHITPSAAQ FNTEKLRWLN QQYIKAADDT RLAGLARPFL IRNGATLDDG
360 370 380 390 400
PDLAAVCALV KERAATIEAL ADAATLFYRV LHPTPELLAQ HVTAEVRPAL
410 420 430 440 450
ADLATRLETL EWERGAISAA FKETLAAHGL KMPKLAMPVR VLVTGEPQTP
460 470
AIDATLELLG REKVLDRLRA AL
Length:472
Mass (Da):52,879
Last modified:October 11, 2005 - v1
Checksum:iE0FDC60D181BA70C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000116 Genomic DNA Translation: AAZ97687.1

Genome annotation databases

EnsemblBacteriaiAAZ97687; AAZ97687; Tbd_1734
KEGGitbd:Tbd_1734

Similar proteinsi

Entry informationi

Entry nameiSYE_THIDA
AccessioniPrimary (citable) accession number: Q3SI46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: October 11, 2005
Last modified: February 28, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome