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Protein

Argininosuccinate synthase

Gene

argG

Organism
Thiobacillus denitrificans (strain ATCC 25259)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei90CitrullineUniRule annotation1
Binding sitei95CitrullineUniRule annotation1
Binding sitei120ATP; via amide nitrogenUniRule annotation1
Binding sitei122AspartateUniRule annotation1
Binding sitei126AspartateUniRule annotation1
Binding sitei126CitrullineUniRule annotation1
Binding sitei127AspartateUniRule annotation1
Binding sitei130CitrullineUniRule annotation1
Binding sitei182CitrullineUniRule annotation1
Binding sitei191CitrullineUniRule annotation1
Binding sitei267CitrullineUniRule annotation1
Binding sitei279CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTDEN292415:G1G56-1867-MONOMER
UniPathwayiUPA00068; UER00113

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Tbd_1849
OrganismiThiobacillus denitrificans (strain ATCC 25259)
Taxonomic identifieri292415 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesThiobacillaceaeThiobacillus
Proteomesi
  • UP000008291 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002639891 – 409Argininosuccinate synthaseAdd BLAST409

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi292415.Tbd_1849

Structurei

3D structure databases

ProteinModelPortaliQ3SHT1
SMRiQ3SHT1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH Bacteria
COG0137 LUCA
HOGENOMiHOG000230093
KOiK01940
OrthoDBiPOG091H01U0

Family and domain databases

CDDicd01999 Argininosuccinate_Synthase, 1 hit
Gene3Di3.40.50.6202 hits
3.90.1260.102 hits
HAMAPiMF_00005 Arg_succ_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001518 Arginosuc_synth
IPR018223 Arginosuc_synth_CS
IPR023434 Arginosuc_synth_type_1_subfam
IPR024074 AS_cat/multimer_dom_body
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR11587 PTHR11587, 1 hit
PfamiView protein in Pfam
PF00764 Arginosuc_synth, 1 hit
TIGRFAMsiTIGR00032 argG, 1 hit
PROSITEiView protein in PROSITE
PS00564 ARGININOSUCCIN_SYN_1, 1 hit
PS00565 ARGININOSUCCIN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q3SHT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDIKKVVLA YSGGLDTSVI LKWLQDTYQC EVVTFTADLG QGEELEPARQ
60 70 80 90 100
KALQFGIKPE QIYIDDLREE FVRDFVFPMF RANTVYEGEY LLGTSIARPL
110 120 130 140 150
IAKRLIEIVN ATGADAICHG ATGKGNDQVR FELGAYALKP DVKVIAPWRE
160 170 180 190 200
WDLLSREKLL AYAESHGIPI DMKHRQGGSP YSMDANLLHI SYEGRHLEDP
210 220 230 240 250
SAEAEEDMWR WTVSPEKAPD AAEYIELTYA KGDVVAIDGQ QMPAHEVLAK
260 270 280 290 300
LNELGGKHGI GRLDLVENRY VGMKSRGCYE TPGGTILLKA HRAIESITLD
310 320 330 340 350
REVAHLKDDL MPRYASLIYN GYWWAPERRA LQVLIDHTQA HVNGTVRLKL
360 370 380 390 400
YKGNVIVVGR DSKNDSLFDS TIATFEDDAG AYDQKDAGGF IKLNALRMRI

AAKLDARRK
Length:409
Mass (Da):45,869
Last modified:December 12, 2006 - v2
Checksum:i687B28A5080F0175
GO

Sequence cautioni

The sequence AAZ97802 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000116 Genomic DNA Translation: AAZ97802.1 Different initiation.
RefSeqiWP_011312361.1, NC_007404.1

Genome annotation databases

EnsemblBacteriaiAAZ97802; AAZ97802; Tbd_1849
KEGGitbd:Tbd_1849

Similar proteinsi

Entry informationi

Entry nameiASSY_THIDA
AccessioniPrimary (citable) accession number: Q3SHT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: February 28, 2018
This is version 76 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome