argG

Functionⁱ

Catalytic activityⁱ

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayⁱ: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:

Ornithine carbamoyltransferase (argF)
Argininosuccinate synthase (argG)
Argininosuccinate lyase (argH)

This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	37	ATP; via amide nitrogen and carbonyl oxygenUniRule annotation	1
Binding siteⁱ	90	CitrullineUniRule annotation	1
Binding siteⁱ	95	CitrullineUniRule annotation	1
Binding siteⁱ	120	ATP; via amide nitrogenUniRule annotation	1
Binding siteⁱ	122	AspartateUniRule annotation	1
Binding siteⁱ	126	AspartateUniRule annotation	1
Binding siteⁱ	126	CitrullineUniRule annotation	1
Binding siteⁱ	127	AspartateUniRule annotation	1
Binding siteⁱ	130	CitrullineUniRule annotation	1
Binding siteⁱ	182	CitrullineUniRule annotation	1
Binding siteⁱ	191	CitrullineUniRule annotation	1
Binding siteⁱ	267	CitrullineUniRule annotation	1
Binding siteⁱ	279	CitrullineUniRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	10 – 18	ATPUniRule annotation		9

GO - Molecular functionⁱ

argininosuccinate synthase activity Source: UniProtKB-EC
ATP binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

arginine biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Ligase
Biological process	Amino-acid biosynthesis, Arginine biosynthesis
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	TDEN292415:G1G56-1867-MONOMER
UniPathwayⁱ	UPA00068; UER00113

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation) Alternative name(s): Citrulline--aspartate ligaseUniRule annotation
Gene namesⁱ	Name:argGUniRule annotation Ordered Locus Names:Tbd_1849
Organismⁱ	Thiobacillus denitrificans (strain ATCC 25259)
Taxonomic identifierⁱ	292415 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Thiobacillaceae › Thiobacillus › Thiobacillus denitrificans
Proteomesⁱ	UP000008291 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00005

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000263989	1 – 409	Argininosuccinate synthaseAdd BLAST		409

Interactionⁱ

Subunit structureⁱ

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGⁱ	292415.Tbd_1849

STRINGⁱ

292415.Tbd_1849

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q3SHT1
SMRⁱ	Q3SHT1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105CDH Bacteria COG0137 LUCA
HOGENOMⁱ	HOG000230093
KEGG Orthology (KO) More...KOⁱ	K01940
Database of Orthologous Groups More...OrthoDBⁱ	POG091H01U0

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01999 Argininosuccinate_Synthase, 1 hit
Gene3Dⁱ	3.40.50.6202 hits 3.90.1260.102 hits
HAMAPⁱ	MF_00005 Arg_succ_synth_type1, 1 hit
InterProⁱ	View protein in InterPro IPR001518 Arginosuc_synth IPR018223 Arginosuc_synth_CS IPR023434 Arginosuc_synth_type_1_subfam IPR024074 AS_cat/multimer_dom_body IPR014729 Rossmann-like_a/b/a_fold
PANTHERⁱ	PTHR11587 PTHR11587, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00764 Arginosuc_synth, 1 hit
TIGRFAMsⁱ	TIGR00032 argG, 1 hit
PROSITEⁱ	View protein in PROSITE PS00564 ARGININOSUCCIN_SYN_1, 1 hit PS00565 ARGININOSUCCIN_SYN_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q3SHT1-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSDIKKVVLA YSGGLDTSVI LKWLQDTYQC EVVTFTADLG QGEELEPARQ 
        60         70         80         90        100
KALQFGIKPE QIYIDDLREE FVRDFVFPMF RANTVYEGEY LLGTSIARPL 
       110        120        130        140        150
IAKRLIEIVN ATGADAICHG ATGKGNDQVR FELGAYALKP DVKVIAPWRE 
       160        170        180        190        200
WDLLSREKLL AYAESHGIPI DMKHRQGGSP YSMDANLLHI SYEGRHLEDP 
       210        220        230        240        250
SAEAEEDMWR WTVSPEKAPD AAEYIELTYA KGDVVAIDGQ QMPAHEVLAK 
       260        270        280        290        300
LNELGGKHGI GRLDLVENRY VGMKSRGCYE TPGGTILLKA HRAIESITLD 
       310        320        330        340        350
REVAHLKDDL MPRYASLIYN GYWWAPERRA LQVLIDHTQA HVNGTVRLKL 
       360        370        380        390        400
YKGNVIVVGR DSKNDSLFDS TIATFEDDAG AYDQKDAGGF IKLNALRMRI 

AAKLDARRK

Length:409

Mass (Da):45,869

Last modified:December 12, 2006 - v2

Checksum:ⁱ687B28A5080F0175

GO

Sequence cautionⁱ

The sequence AAZ97802 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000116 Genomic DNA Translation: AAZ97802.1 Different initiation.
NCBI Reference Sequences More...RefSeqⁱ	WP_011312361.1, NC_007404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAZ97802; AAZ97802; Tbd_1849
KEGGⁱ	tbd:Tbd_1849

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q3SHT1	A0A258TLT0 A0A257WTN1 A0A1Q4GES3 A0A258F574 A0A257XUD8 A0A1Q4ENR9 A0A259F7T5 A0A1E4KQN0 A0A1E4KE46 A0A1G0NB80 +17	Hydrogenophilales bacterium 28-61-23 Hydrogenophilales bacterium 12-63-5 Thiobacillus sp. 63-78 Hydrogenophilales bacterium 32-62-9 Hydrogenophilales bacterium 12-61-10 Thiobacillus sp. 0-1251 Hydrogenophilales bacterium 17-64-65 Thiobacillus sp. SCN 63-374 Hydrogenophilales bacterium RIFOXYD1_FULL_62_11 Hydrogenophilaceae bacterium CG1_02_62_390 And more	409	UniRef90_Q3SHT1	Cluster: Argininosuccinate synthase	28

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q3SHT1	A0A258TLT0 A0A1J4Z4R5 A0A2M7I6J4 A0A259ER04 A0A106BNI8 A0A257WTN1 A0A1G0NB80 UPI000360AE62 A0A1E4KQN0 A0A1M3HER2 +31	Hydrogenophilales bacterium 28-61-23 Hydrogenophilaceae bacterium CG1_02_62_390 Hydrogenophilales bacterium CG12_big_fil_rev_8_21_14_0_65_61_21 Hydrogenophilales bacterium 17-64-11 Thiobacillus denitrificans Hydrogenophilales bacterium 12-63-5 Hydrogenophilales bacterium RIFOXYD1_FULL_62_11 Thiobacillus thioparus Thiobacillus sp. SCN 63-374 Thiobacillus sp. 65-1059 And more	409	UniRef50_Q3SHT1	Cluster: Argininosuccinate synthase	42

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000116 Genomic DNA Translation: AAZ97802.1 Different initiation.
NCBI Reference Sequences More...RefSeqⁱ	WP_011312361.1, NC_007404.1

3D structure databases

ProteinModelPortalⁱ	Q3SHT1
SMRⁱ	Q3SHT1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	292415.Tbd_1849

STRINGⁱ

292415.Tbd_1849

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAZ97802; AAZ97802; Tbd_1849
KEGGⁱ	tbd:Tbd_1849

Phylogenomic databases

eggNOGⁱ	ENOG4105CDH Bacteria COG0137 LUCA
HOGENOMⁱ	HOG000230093
KEGG Orthology (KO) More...KOⁱ	K01940
Database of Orthologous Groups More...OrthoDBⁱ	POG091H01U0

Enzyme and pathway databases

UniPathwayⁱ	UPA00068; UER00113
BioCycⁱ	TDEN292415:G1G56-1867-MONOMER

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01999 Argininosuccinate_Synthase, 1 hit
Gene3Dⁱ	3.40.50.6202 hits 3.90.1260.102 hits
HAMAPⁱ	MF_00005 Arg_succ_synth_type1, 1 hit
InterProⁱ	View protein in InterPro IPR001518 Arginosuc_synth IPR018223 Arginosuc_synth_CS IPR023434 Arginosuc_synth_type_1_subfam IPR024074 AS_cat/multimer_dom_body IPR014729 Rossmann-like_a/b/a_fold
PANTHERⁱ	PTHR11587 PTHR11587, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00764 Arginosuc_synth, 1 hit
TIGRFAMsⁱ	TIGR00032 argG, 1 hit
PROSITEⁱ	View protein in PROSITE PS00564 ARGININOSUCCIN_SYN_1, 1 hit PS00565 ARGININOSUCCIN_SYN_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ASSY_THIDA
Accessionⁱ	Q3SHT1Primary (citable) accession number: Q3SHT1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
	Last sequence update:	December 12, 2006
	Last modified:	February 28, 2018
	This is version 76 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q3SHT1 (ASSY_THIDA)

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Argininosuccinate synthase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-arginine biosynthesis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: L-arginine biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ