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Q3SHS3

- SYI_THIDA

UniProt

Q3SHS3 - SYI_THIDA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thiobacillus denitrificans (strain ATCC 25259)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
Binding sitei612 – 6121ATPUniRule annotation
Metal bindingi892 – 8921ZincUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi912 – 9121ZincUniRule annotation
Metal bindingi915 – 9151ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTDEN292415:GHWG-1900-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Tbd_1857
OrganismiThiobacillus denitrificans (strain ATCC 25259)
Taxonomic identifieri292415 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaHydrogenophilalesHydrogenophilaceaeThiobacillus
ProteomesiUP000008291: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929Isoleucine--tRNA ligasePRO_0000098495Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi292415.Tbd_1857.

Structurei

3D structure databases

ProteinModelPortaliQ3SHS3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi609 – 6135"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGHRVHY.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3SHS3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPDYKSTLNL PDTPFPMRGD LAKREPGWVK SWQEKKRYEA IRAAAAGRPK
60 70 80 90 100
FILHDGPPYA NGDIHIGHAV NKILKDIIVK AKTLSGFDAP YVPGWDCHGL
110 120 130 140 150
PIELQVEKTH GKDIPPAKFR ELCRAYAAEQ IERQKADFIR LGVLGDWSNP
160 170 180 190 200
YRTMDFQFEA DTLRVLGQIQ QAGFLYQGAK PVHWCVDCGS ALAEAEVEYE
210 220 230 240 250
DKNSPAIDVG FAVADRADLA RRFDVAAIDT PVQIVIWTTT PWTLPANQAV
260 270 280 290 300
ALHPDFPYTL VRTARGLLVL AESLREAALV RYGLADGAEI LAHTTGQMLE
310 320 330 340 350
GLPLWHPFQD RQVPVIVGEH VTADAGTGAV HTAPGHGLDD YVVGSRYGLK
360 370 380 390 400
VDNPVGDDGR FYASVPLVGG MSIWQANPLI VETLEASGAL LAHEKLLHSY
410 420 430 440 450
PHCWRHKTPI IFRATRQWFI GMDSAGQDSG VRDQGATLRE QAMKAVEATQ
460 470 480 490 500
FFPHWGRARL EAMIRNRPDW CVSRQRNWGV PMPFFTHRET GALHPRTTEL
510 520 530 540 550
LEIVAQRVET AGIEAWFALD PAELLGDDAA HYDKTGHTLD VWFDSGVTHA
560 570 580 590 600
CVLKRRSELA HPADLYLEGS DQHRGWFQSS LLTGCATDGR APYDALLTHG
610 620 630 640 650
FVVDGKGHKM SKSKGNVIAP QKVMDQYGAD ILRLWVATTD YSGELSISDE
660 670 680 690 700
ILKRVVEGYR RIRNTLKFLL ANLSDFDPRE HAMSVDEWLE IDRYALAMTR
710 720 730 740 750
RLQGELQRHY DAYEFHFIVQ KLQSFCSEDL GGFYLDILKD RLYTSAGDSR
760 770 780 790 800
ARRAAQNALH HLTHALVRWM APILSFTGEE VWTQLADADD SVFLHTRHVL
810 820 830 840 850
PEQGGEDELL ERWARIRALR AEVQKELETV RVAGAIGSSL QAEVTLHATP
860 870 880 890 900
STAALLSSLA DDLRFVLITS QARVVGADAD RVEVAPSAAK KCDRCWHYRD
910 920
DVDAHPEHPG LCGRCVSNLF GDGEARRHA
Length:929
Mass (Da):103,660
Last modified:October 11, 2005 - v1
Checksum:i1EB80F7ADFC9BA65
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000116 Genomic DNA. Translation: AAZ97810.1.
RefSeqiYP_315615.1. NC_007404.1.

Genome annotation databases

EnsemblBacteriaiAAZ97810; AAZ97810; Tbd_1857.
GeneIDi3671522.
KEGGitbd:Tbd_1857.
PATRICi23969885. VBIThiDen82923_1836.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000116 Genomic DNA. Translation: AAZ97810.1 .
RefSeqi YP_315615.1. NC_007404.1.

3D structure databases

ProteinModelPortali Q3SHS3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 292415.Tbd_1857.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ97810 ; AAZ97810 ; Tbd_1857 .
GeneIDi 3671522.
KEGGi tbd:Tbd_1857.
PATRICi 23969885. VBIThiDen82923_1836.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGHRVHY.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TDEN292415:GHWG-1900-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans."
    Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W., Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P.
    J. Bacteriol. 188:1473-1488(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25259.

Entry informationi

Entry nameiSYI_THIDA
AccessioniPrimary (citable) accession number: Q3SHS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 11, 2005
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3