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Q3SHS3

- SYI_THIDA

UniProt

Q3SHS3 - SYI_THIDA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thiobacillus denitrificans (strain ATCC 25259)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (11 Oct 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
    Binding sitei612 – 6121ATPUniRule annotation
    Metal bindingi892 – 8921ZincUniRule annotation
    Metal bindingi895 – 8951ZincUniRule annotation
    Metal bindingi912 – 9121ZincUniRule annotation
    Metal bindingi915 – 9151ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciTDEN292415:GHWG-1900-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Tbd_1857
    OrganismiThiobacillus denitrificans (strain ATCC 25259)
    Taxonomic identifieri292415 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaHydrogenophilalesHydrogenophilaceaeThiobacillus
    ProteomesiUP000008291: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 929929Isoleucine--tRNA ligasePRO_0000098495Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi292415.Tbd_1857.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3SHS3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi609 – 6135"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiLGHRVHY.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3SHS3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPDYKSTLNL PDTPFPMRGD LAKREPGWVK SWQEKKRYEA IRAAAAGRPK    50
    FILHDGPPYA NGDIHIGHAV NKILKDIIVK AKTLSGFDAP YVPGWDCHGL 100
    PIELQVEKTH GKDIPPAKFR ELCRAYAAEQ IERQKADFIR LGVLGDWSNP 150
    YRTMDFQFEA DTLRVLGQIQ QAGFLYQGAK PVHWCVDCGS ALAEAEVEYE 200
    DKNSPAIDVG FAVADRADLA RRFDVAAIDT PVQIVIWTTT PWTLPANQAV 250
    ALHPDFPYTL VRTARGLLVL AESLREAALV RYGLADGAEI LAHTTGQMLE 300
    GLPLWHPFQD RQVPVIVGEH VTADAGTGAV HTAPGHGLDD YVVGSRYGLK 350
    VDNPVGDDGR FYASVPLVGG MSIWQANPLI VETLEASGAL LAHEKLLHSY 400
    PHCWRHKTPI IFRATRQWFI GMDSAGQDSG VRDQGATLRE QAMKAVEATQ 450
    FFPHWGRARL EAMIRNRPDW CVSRQRNWGV PMPFFTHRET GALHPRTTEL 500
    LEIVAQRVET AGIEAWFALD PAELLGDDAA HYDKTGHTLD VWFDSGVTHA 550
    CVLKRRSELA HPADLYLEGS DQHRGWFQSS LLTGCATDGR APYDALLTHG 600
    FVVDGKGHKM SKSKGNVIAP QKVMDQYGAD ILRLWVATTD YSGELSISDE 650
    ILKRVVEGYR RIRNTLKFLL ANLSDFDPRE HAMSVDEWLE IDRYALAMTR 700
    RLQGELQRHY DAYEFHFIVQ KLQSFCSEDL GGFYLDILKD RLYTSAGDSR 750
    ARRAAQNALH HLTHALVRWM APILSFTGEE VWTQLADADD SVFLHTRHVL 800
    PEQGGEDELL ERWARIRALR AEVQKELETV RVAGAIGSSL QAEVTLHATP 850
    STAALLSSLA DDLRFVLITS QARVVGADAD RVEVAPSAAK KCDRCWHYRD 900
    DVDAHPEHPG LCGRCVSNLF GDGEARRHA 929
    Length:929
    Mass (Da):103,660
    Last modified:October 11, 2005 - v1
    Checksum:i1EB80F7ADFC9BA65
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000116 Genomic DNA. Translation: AAZ97810.1.
    RefSeqiYP_315615.1. NC_007404.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ97810; AAZ97810; Tbd_1857.
    GeneIDi3671522.
    KEGGitbd:Tbd_1857.
    PATRICi23969885. VBIThiDen82923_1836.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000116 Genomic DNA. Translation: AAZ97810.1 .
    RefSeqi YP_315615.1. NC_007404.1.

    3D structure databases

    ProteinModelPortali Q3SHS3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 292415.Tbd_1857.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ97810 ; AAZ97810 ; Tbd_1857 .
    GeneIDi 3671522.
    KEGGi tbd:Tbd_1857.
    PATRICi 23969885. VBIThiDen82923_1836.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi LGHRVHY.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci TDEN292415:GHWG-1900-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans."
      Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W., Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P.
      J. Bacteriol. 188:1473-1488(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25259.

    Entry informationi

    Entry nameiSYI_THIDA
    AccessioniPrimary (citable) accession number: Q3SHS3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3