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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thiobacillus denitrificans (strain ATCC 25259)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei568Aminoacyl-adenylateUniRule annotation1
Binding sitei612ATPUniRule annotation1
Metal bindingi892ZincUniRule annotation1
Metal bindingi895ZincUniRule annotation1
Metal bindingi912ZincUniRule annotation1
Metal bindingi915ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTDEN292415:G1G56-1877-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Tbd_1857
OrganismiThiobacillus denitrificans (strain ATCC 25259)
Taxonomic identifieri292415 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesThiobacillaceaeThiobacillus
Proteomesi
  • UP000008291 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984951 – 929Isoleucine--tRNA ligaseAdd BLAST929

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi292415.Tbd_1857

Structurei

3D structure databases

ProteinModelPortaliQ3SHS3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi609 – 613"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.6202 hits
3.90.740.101 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q3SHS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDYKSTLNL PDTPFPMRGD LAKREPGWVK SWQEKKRYEA IRAAAAGRPK
60 70 80 90 100
FILHDGPPYA NGDIHIGHAV NKILKDIIVK AKTLSGFDAP YVPGWDCHGL
110 120 130 140 150
PIELQVEKTH GKDIPPAKFR ELCRAYAAEQ IERQKADFIR LGVLGDWSNP
160 170 180 190 200
YRTMDFQFEA DTLRVLGQIQ QAGFLYQGAK PVHWCVDCGS ALAEAEVEYE
210 220 230 240 250
DKNSPAIDVG FAVADRADLA RRFDVAAIDT PVQIVIWTTT PWTLPANQAV
260 270 280 290 300
ALHPDFPYTL VRTARGLLVL AESLREAALV RYGLADGAEI LAHTTGQMLE
310 320 330 340 350
GLPLWHPFQD RQVPVIVGEH VTADAGTGAV HTAPGHGLDD YVVGSRYGLK
360 370 380 390 400
VDNPVGDDGR FYASVPLVGG MSIWQANPLI VETLEASGAL LAHEKLLHSY
410 420 430 440 450
PHCWRHKTPI IFRATRQWFI GMDSAGQDSG VRDQGATLRE QAMKAVEATQ
460 470 480 490 500
FFPHWGRARL EAMIRNRPDW CVSRQRNWGV PMPFFTHRET GALHPRTTEL
510 520 530 540 550
LEIVAQRVET AGIEAWFALD PAELLGDDAA HYDKTGHTLD VWFDSGVTHA
560 570 580 590 600
CVLKRRSELA HPADLYLEGS DQHRGWFQSS LLTGCATDGR APYDALLTHG
610 620 630 640 650
FVVDGKGHKM SKSKGNVIAP QKVMDQYGAD ILRLWVATTD YSGELSISDE
660 670 680 690 700
ILKRVVEGYR RIRNTLKFLL ANLSDFDPRE HAMSVDEWLE IDRYALAMTR
710 720 730 740 750
RLQGELQRHY DAYEFHFIVQ KLQSFCSEDL GGFYLDILKD RLYTSAGDSR
760 770 780 790 800
ARRAAQNALH HLTHALVRWM APILSFTGEE VWTQLADADD SVFLHTRHVL
810 820 830 840 850
PEQGGEDELL ERWARIRALR AEVQKELETV RVAGAIGSSL QAEVTLHATP
860 870 880 890 900
STAALLSSLA DDLRFVLITS QARVVGADAD RVEVAPSAAK KCDRCWHYRD
910 920
DVDAHPEHPG LCGRCVSNLF GDGEARRHA
Length:929
Mass (Da):103,660
Last modified:October 11, 2005 - v1
Checksum:i1EB80F7ADFC9BA65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000116 Genomic DNA Translation: AAZ97810.1
RefSeqiWP_011312369.1, NC_007404.1

Genome annotation databases

EnsemblBacteriaiAAZ97810; AAZ97810; Tbd_1857
KEGGitbd:Tbd_1857

Similar proteinsi

Entry informationi

Entry nameiSYI_THIDA
AccessioniPrimary (citable) accession number: Q3SHS3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 11, 2005
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome