Q3SH82 (PANC_THIDA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantothenate synthetase
Short name=PS
EC=6.3.2.1

Alternative name(s):
Pantoate--beta-alanine ligase
Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	Tbd_2054

Organism

Thiobacillus denitrificans (strain ATCC 25259) [Complete proteome] [HAMAP]

Taxonomic identifier

292415 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Hydrogenophilales › Hydrogenophilaceae › Thiobacillus › Thiobacillus denitrificans

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP-Rule MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP-Rule MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP-Rule MF_00158
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP-Rule MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Pantothenate synthetase HAMAP-Rule MF_00158		PRO_0000305568
Regions
	Nucleotide binding	26 – 33	8	ATP By similarity
	Nucleotide binding	144 – 147	4	ATP By similarity
	Nucleotide binding	181 – 184	4	ATP By similarity
Sites
	Active site	33	1	Proton donor By similarity
	Binding site	57	1	Beta-alanine By similarity
	Binding site	57	1	Pantoate By similarity
	Binding site	150	1	Pantoate By similarity
	Binding site	173	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3SH82 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: EE0C25ABC7A4F476	FASTA	283	30,117
	10 20 30 40 50 60 MQVVHTVADL RAALSEADRS AFVPTMGNLH AGHVSLVELA KRHGGPVVAS IFVNPLQFGA 70 80 90 100 110 120 GEDFERYPRT LAADCDKLAE AGCDLVFAPD TSALYPVAQT FRVDVPAALA EDLCGAFRPG 130 140 150 160 170 180 HFAGVATVVL KLFNLVRPQV AVFGRKDYQQ LLVVREMVRQ FNLPIDIVAG ATLRDPDGLA 190 200 210 220 230 240 MSSRNGYLSA AERAQAPQLQ RELAAIVAAI DGGARNFAAL AAAARQRLTG AGWRVDYVEI 250 260 270 280 RDAESLKTAT QETEALVVLA AAWLGQTRLI DNLEVTSAAG IAA

References

[1]

"The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans." Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W., Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P. J. Bacteriol. 188:1473-1488(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25259.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000116 Genomic DNA. Translation: AAZ98007.1.
RefSeq	YP_315812.1. NC_007404.1.
3D structure databases
HSSP	HSSP built from PDB template 2A7X based on UniProtKB P0A5R0.
ProteinModelPortal	Q3SH82.
ModBase	Search...
Protein-protein interaction databases
STRING	292415.Tbd_2054.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ98007; AAZ98007; Tbd_2054.
GeneID	3672225.
KEGG	tbd:Tbd_2054.
PATRIC	23970283. VBIThiDen82923_2032.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0414.
HOGENOM	HOG000175516.
KO	K01918.
OMA	EDFGSYP.
ProtClustDB	PRK00380.
Enzyme and pathway databases
UniPathway	UPA00028; UER00005.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00158. PanC.
InterPro	IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR21299:SF1. PTHR21299:SF1. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_THIDA

Accession

Primary (citable) accession number: Q3SH82

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 11, 2005
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program