Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q3SH78 (GLGB_THIDA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Tbd_2058
OrganismThiobacillus denitrificans (strain ATCC 25259) [Complete proteome] [HAMAP]
Taxonomic identifier292415 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaHydrogenophilalesHydrogenophilaceaeThiobacillus

Protein attributes

Sequence length740 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7407401,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260711

Sites

Active site4191Nucleophile By similarity
Active site4721Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3SH78 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 0DACE2E56940ABF5

FASTA74084,263
        10         20         30         40         50         60 
MRKLVEATQA GSEASVALAP SAPIQRLQAG LHHDPFEVLG VHVQPDGSKL VRAFLPAAEA 

        70         80         90        100        110        120 
VEVAGVRMTR VPGTDCFERL LPPGTALEAH PLLTWQDKRS GAWQRLRSPY SFAPQLGEMD 

       130        140        150        160        170        180 
LYLFGEGRHF EIWKVLGARV KTVDGVTGCL FAVWAPAVLR VSVVGDFNDW DGRRHPMRCR 

       190        200        210        220        230        240 
GVSGVWELFI PGLEAGHAYK YEILGRHGER VTKTDPYARQ MFLRPETTSR VPDERPYAWG 

       250        260        270        280        290        300 
DAEWLAARTR FDWQHRPMSV YEVHPGSWRR RADGSFYSWR ELTAELIPYV RDLGYTHIEL 

       310        320        330        340        350        360 
LPVAEHPFDA SWGYQVSGYY AATARFGSPD DLRAFVDACH QAGLGVLLDW VPGHFPKDDF 

       370        380        390        400        410        420 
ALARFTGEPL YEHADPRRGE HQDWGTLVFD FGRNEVRNFL VANALYWLEE FHIDGLRVDA 

       430        440        450        460        470        480 
VASMLYLDYS RRHGEWLPNQ HGGRENLEAI HFLHEVNAEV HARFPGAITI AEESTAWPAV 

       490        500        510        520        530        540 
SRPIELGGLG FSMKWNMGWM NDTLDYIEKE PVYRKYQHNQ LTFSQMYAWS ENFVLPLSHD 

       550        560        570        580        590        600 
EVVHLKKSLL DKMPGDRWQR FANLRLLYAW QYAHPGKKLL FMGGEFGQWN EWREAGQLDW 

       610        620        630        640        650        660 
VLLGFPEHDG IRALLRDLNR LYRDEAALHF WDFDPRGFRW IDCHDADQSV LSLVREGPDG 

       670        680        690        700        710        720 
APPIVVLLNF TPVPRHGYRI GVPRAGAWCE VLNSDSMYYG GSNLGNGKPL YPAAVPWMGF 

       730        740 
GQSIEVTLPP LGAIFLKPCP 

« Hide

References

[1]"The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans."
Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W., Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P.
J. Bacteriol. 188:1473-1488(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25259.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000116 Genomic DNA. Translation: AAZ98011.1.
RefSeqYP_315816.1. NC_007404.1.

3D structure databases

ProteinModelPortalQ3SH78.
SMRQ3SH78. Positions 128-739.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING292415.Tbd_2058.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ98011; AAZ98011; Tbd_2058.
GeneID3672229.
KEGGtbd:Tbd_2058.
PATRIC23970291. VBIThiDen82923_2036.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAISEGRHE.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycTDEN292415:GHWG-2104-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_THIDA
AccessionPrimary (citable) accession number: Q3SH78
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 11, 2005
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries