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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Thiobacillus denitrificans (strain ATCC 25259)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60S-adenosyl-L-methionineUniRule annotation1
Binding sitei85S-adenosyl-L-methionineUniRule annotation1
Binding sitei112S-adenosyl-L-methionineUniRule annotation1
Active sitei135By similarity1
Binding sitei135S-adenosyl-L-methionineUniRule annotation1
Binding sitei139SubstrateUniRule annotation1
Binding sitei171SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTDEN292415:G1G56-2152-MONOMER
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Tbd_2125
OrganismiThiobacillus denitrificans (strain ATCC 25259)
Taxonomic identifieri292415 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesThiobacillaceaeThiobacillus
Proteomesi
  • UP000008291 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002292061 – 227tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST227

Interactioni

Protein-protein interaction databases

STRINGi292415.Tbd_2125

Structurei

3D structure databases

ProteinModelPortaliQ3SH11
SMRiQ3SH11
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni206 – 209Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000073968
KOiK03439
OMAiPDPWHKS
OrthoDBiPOG091H015P

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q3SH11-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTADGGARPR IRSYVLRAGR VGSGQARALV ELGPRFVLPY QPELLDLDAV
60 70 80 90 100
FARVAPRVLE IGFGMGEGLA ETAASHPEID YIGVEVHTPG VGALLKQLGE
110 120 130 140 150
RELGNVRVIQ HDAVEVLTAM LAPATLAGIH IFFPDPWHKK RHHKRRLIQA
160 170 180 190 200
PLVELLASRL APGGYIHLAT DWQDYAEQML VVLEAEAQLE NTAAAYVPRP
210 220
DTRPLTKFEQ RGIRLGHGVW DLVFRRR
Length:227
Mass (Da):25,135
Last modified:October 11, 2005 - v1
Checksum:i69729B5AECFF92FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000116 Genomic DNA Translation: AAZ98078.1
RefSeqiWP_011312637.1, NC_007404.1

Genome annotation databases

EnsemblBacteriaiAAZ98078; AAZ98078; Tbd_2125
KEGGitbd:Tbd_2125

Similar proteinsi

Entry informationi

Entry nameiTRMB_THIDA
AccessioniPrimary (citable) accession number: Q3SH11
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 11, 2005
Last modified: February 28, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome