Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Thiobacillus denitrificans (strain ATCC 25259)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei623ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Tbd_2445
OrganismiThiobacillus denitrificans (strain ATCC 25259)
Taxonomic identifieri292415 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaHydrogenophilalesHydrogenophilaceaeThiobacillus
Proteomesi
  • UP000008291 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000094581 – 857Leucine--tRNA ligaseAdd BLAST857

Proteomic databases

PRIDEiQ3SG58.

Interactioni

Protein-protein interaction databases

STRINGi292415.Tbd_2445.

Structurei

3D structure databases

ProteinModelPortaliQ3SG58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 52"HIGH" regionAdd BLAST11
Motifi620 – 624"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
HOGENOMiHOG000200747.
KOiK01869.
OMAiHRFIKRL.
OrthoDBiPOG091H01JZ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 3 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3SG58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEKYLPSEI ERAGQARWTA DQTYRAADAS DRPKYYCLSM FPYPSGKLHM
60 70 80 90 100
GHVRNYTIGD VLARYHALRG FNVMQPMGWD AFGLPAENAA IANGVPPAQW
110 120 130 140 150
TYANIDHMRT QLQALGFAID WSRELATCKP DYYRWEQWLF TRLFEKGVIY
160 170 180 190 200
KKMATVNWDP VDQTVLANEQ VIDGRGWRSG ALVEKRDIPM YFFRITQYAD
210 220 230 240 250
ELLSGLDTLP GWPERVKTMQ ANWIGKSTGV RLAFPYELDG SQEKLWVFTT
260 270 280 290 300
RADTLMGVTF VAVAAEHPLA ARAAENNPEL AAFVAECKQG SVAEADMATM
310 320 330 340 350
EKKGMDTGFK VTHPLTGEEV PVWVGNYVLM SYGEGAVMAV PAHDERDFGF
360 370 380 390 400
AKKYDLPIKQ VIGVDGETFS LDAWAEWYGD KTRGQCVNSG KYDRLGYEAA
410 420 430 440 450
VDAIAADLAA KGLGEKKTQF RLRDWGISRQ RYWGCPIPII HCETCGDVPV
460 470 480 490 500
PAEQLPVVLP EDVVPDGSGN PLNKRADFVN CTCPACGAPA RRETDTMDTF
510 520 530 540 550
VESSWYYARY ACPDYADGML DARADQWLPV DQYIGGIEHA ILHLLYARFF
560 570 580 590 600
HKLMRDEGLV ASDEPFANLL TQGMVVADTY YREDAGGKKT WFNPADVETK
610 620 630 640 650
DGVATLRADG KPVVVGGTEK MSKSKNNGVD PQALIDQYGA DTARLFTMFA
660 670 680 690 700
APPEQSLEWS DAGVEGAHRF LRRLWKTVYE HVQAGPVVTR AGGALPEPLK
710 720 730 740 750
ALRRQLHQTI QKVGDDIERR KQFNTAIAAV MELMNALAKL DGMDADTRAV
760 770 780 790 800
RQETLEAVAV LLAPIVPHVG EAIHAELRPG AAMRWPEVDA AALVQDEIEL
810 820 830 840 850
MLQVNGKLRG QIRVAAGADK PAIEAAALAS EAVQKYLAGQ TPKKVVVVPG

RLVNIVA
Length:857
Mass (Da):94,939
Last modified:October 11, 2005 - v1
Checksum:i792609177FB4A315
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000116 Genomic DNA. Translation: AAZ98398.1.
RefSeqiWP_011312957.1. NC_007404.1.

Genome annotation databases

EnsemblBacteriaiAAZ98398; AAZ98398; Tbd_2445.
KEGGitbd:Tbd_2445.
PATRICi23971085. VBIThiDen82923_2429.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000116 Genomic DNA. Translation: AAZ98398.1.
RefSeqiWP_011312957.1. NC_007404.1.

3D structure databases

ProteinModelPortaliQ3SG58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi292415.Tbd_2445.

Proteomic databases

PRIDEiQ3SG58.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ98398; AAZ98398; Tbd_2445.
KEGGitbd:Tbd_2445.
PATRICi23971085. VBIThiDen82923_2429.

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
HOGENOMiHOG000200747.
KOiK01869.
OMAiHRFIKRL.
OrthoDBiPOG091H01JZ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 3 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYL_THIDA
AccessioniPrimary (citable) accession number: Q3SG58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.